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(((000777>>>EEELLKRRUYY^__eeejkjpppvvv{{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6f38ced6e2914d50a65912cfa7758164Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-09_01-47_07603130Seq16819134911_134911mfagan96-Well###"#""######"#"#"##"##########################"""##################"###############################"#####"######"#########"########################"###"#################"############################"#########################################"#######"#######################""#####"######"###############"##"##"##############"###"######################"#"#"####"###################"###"####################"#"#########"#######################"#############"##########"######"####################"########"########""##########"#############################""##""######"############################################"############"################################"########"################################################################"#############"###########################"####################"###############"####"######################"######"#############"##"##########"####################################""#"##""########"####"##########################################"######################################"##########"#########################"##################"##################################################"#"###############"###############################"###"##################"######################"###"##""######"####"################ K1321_A1_Indeterminate_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z}zvrmida][YWVTRQNLHD@<9655568:<<<:7669=BFGGD@<:9:<?BCB@<9667:<===;:9:=BIR[dmv6N`kruy~yeO;* sh`ZVRNH@7/)&&&'&$!"$&&%$#""! 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"),,($ 6b"OE%[1$'.0+!        7 ^i1Fn4   0e/#M(  Trvg/ G0AW`}?#"    E'wIEvq=h5U+%(&!6?"X1&)/1-$   #&% @'M t/R"_:156,P7-kj,Eq5 ?&,(b(        0hbrv!D,_5''+,)!   #jeb/ai-XS.b*   A(I%M"  dA +Y@  G*4M[u4   7I@&W(   ,qGNE  %e28x=P<Ym' F"X$U(   !dL6mf$=q8  QFe(V* VC:tm)?q7  IQMg='    ,n< rtJ4,*'" 2oUw*6n^uX2"      JLdF"vF-##$$W82{[kA,%!     EdP\7%  I\t~*E."tB'  CD{&O/     Y'XjB|'Rwg'ZgE5-'        =O(fqH{N5'  FX"{I.! 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A[+3H!JU_'EO`+T F#)UN+36@O]C2adZF7>a7X+Nyo8 '-8v/i0Wl<hSua9pBM/s R@hUfJXQ{,M"UCt#*1{{2%;e;;2t>sQ]4O34 r/ SP   ?  \Ia.8GF2YXQs$3\,@Cg)  8ayu+~om*n&B#zMRoWyhhg4xSXw{ \^U :AqBALEhjLa g*j i; (Z_>k{?-3t @j.yVGN6[`="})yjnL5Ci#S2y\m.C\0}265:9Z<$v1x$u l -oPbH HU3md_(>Lbtwudlr~`RR{HJV:F=;:.45@C&BD'&7+& "                              % !"#'    +#"!"" *"'&)!$*+0..3/+)2 /&9%1,57&*# DigX3K3dspF9x?",tB%TRpiOdSn(\8: pndPpmSO~8Pz;XW4u.p<JEs>oO*=w*kRF ldIE' q 9&I@ Ab7dLGd{l8+ 6lXUxY^^y=!dgXaw(*l_ldsmQU>P+y!*J{NC4<]=We%B_(dz[@cPBP8dHq`pm pP~Bt->n/sUp,pv  ?c%!{1o ,  NEytQ K#Ln r/^)6;_8!+AA($$K3'(@[!@;,A *&+)$-1,&0"%:(133:a&'>0J5,3I&&+0) &+#520$ !+ &3)%6(/$>,$ 04"##..729DKBM.$"+#,, H &L3%-'!(844 <Lc7B\( 1-=< O$C.:! 1%10$#3,.C8 J=&?6+)!+5$8,#& 2')=24 :'$"&-&3"*(#7,FR I5"$&!))+"46(1H-4F 5+,%+':FWo9Y7?(%,D<&'"$9'),$"."".C.)'("Lb'( #05J"!;)7".-&(H != 1Ao" .-'9,#"D.5"& #$9!W+$(,(?ND;.@x3"CCAAAAAATCCAAAGCTATACATAGCCAATCGAATATACAAACTACATTTGGATTATAACACCTAACCAGAGAACACCGACCCTCGTGGACTAAATCTCTCTCACCTCAAATCCAGCGAGACGCCCATCGAATTTTGAAATTACAGGCGCCGGATATCCTCACCTCTCCTACAGACAGAGCGCGCGGACCTCCGACCTAACGTACTTCACTTCCTCGGAAGGTACTCTTGTGAGTACCACACCTTCCTCCACGGGGAAGTAGGGAAAATCCGCCTCAGGAACTAGTTGGTTAGCCTCCTCTGGAAGGGGAAGCGCTACTTCAGCGCCTCCTTCCGTGGCAAGAACGAAGCTCGGGACTTCATCCCTTCTTTGTGGTGTTCGTGTAAGAATAGGATAATCAGGAGCGGGAGCCTTTTGTTCATCATGAATCATTTCTTGTAGGAGAAGTGATTCTAGGGGATCCTTCGATATATGTAGATTCGTTACCACATTCTGAAGAATATCATGAGTCTAGATATTCTACATGACTTCATCGGAGGATTCGACTCTATCAAGCGTCTGAGGATTAATGACATAATATAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 03:33:51 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSq[BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ={TDATA ={ЂDATA ={LrDATA ={bDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISDZP1AMG[SP1RLGVP1WDG_P2AMGhP2BAGGmP2RLGdoPBASGGFPBASGGDPCONGGT~PCONGGR7PDMFTrPDMFDqPLOCGMPLOCGIPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%DRSCAN@SMED SMLtSMPL!!TQSPACAgݰSPACDjSPACAgݰSVER4.0SVER qPSVER,,/Scal@Scan@TUBEA1Tmpr<UserphARphCHDphDYbigphQLcphTRphTR9This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSq[BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ={TDATA ={ЂDATA ={LrDATA ={bDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//vGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISDZP1AMG[SP1RLGVP1WDG_P2AMGhP2BAGGmP2RLGdoPBASGGFPBASGGDPCONGGT~PCONGGR7PDMFTrPDMFDqPLOCGMPLOCGIPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND  RUND  RUND  RUND  RUNT /RUNT !RUNT  2RUNT ! Rate 0RunN""J:S/N%DRSCAN@SMED SMLtSMPL!!TQSPACAgݰSPACDjSPACAgݰSVER4.0SVER qPSVER,,/Scal@Scan@TUBEA1Tmpr<UserphARphCHDphDYbigphQLcphTRphTR!q*D]{SmP{QJHeXSbNfP>A0&&e`r/zapM.=!@D:Z>K>D`Vd96M8@/|thLG^r@uPDsvA;DmwC&Y?^dIrLei;(F}K7T]Zj7y!R}*Z8y0Pajeoi@LaiY2]C0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4\KUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^bAmK7^d}|}{B.@k5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQEO=4gHO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX>V