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  (((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 082e5a23ca9a4bd2b39be35a4aadf7d1Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"########## 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NTQS    0HjhSwBEtni`TE5(  *7DPXZWOD6* }@33AA@bAKB.bcpKB_3730_POP7_BDTv3.mobtermGAAGTGTGGCATAGCTGGTGTTAGGATTATAAATTGACTTACTACACCCCGGAGTACGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAGCCCGGGGTTCCGCCTGAAGAAGCAGGGGCTGCAGTAGCTGCGGAATCTTCTACTGGTACATGGACAACCGTTTGGACTGATGGACTTACCAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCCGTTCCTGGGGAGGCAGATCAATATATCTGTTATATAGCTTATCCATTAGACCTATTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAACGTGTTTGGTTTCAAAGCCCTACGCGCTCTACGTTTGGAGGATCTACGAATTCCTCCTAGTTATTCAAAAACTTTCCAAGGTCCGCCTCACGGTATCCAAGTTGAAAGGGATAAGTTGAACAAGTATGGTCGTCCTTTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCAAAAAATTATGGTAGAGCGTGTTATGAGTGTCTACGCGGTGGATTGGAATTTTAACAAACAGAAGTGTGGCATAGCTGGTGTTAGGATTATAAATTGACTTACTACACCCCGGAGTACGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAGCCCGGGGTTCCGCCTGAAGAAGCAGGGGCTGCAGTAGCTGCGGAATCTTCTACTGGTACATGGACAACCGTTTGGACTGATGGACTTACCAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCCGTTCCTGGGGAGGCAGATCAATATATCTGTTATATAGCTTATCCATTAGACCTATTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAACGTGTTTGGTTTCAAAGCCCTACGCGCTCTACGTTTGGAGGATCTACGAATTCCTCCTAGTTATTCAAAAACTTTCCAAGGTCCGCCTCACGGTATCCAAGTTGAAAGGGATAAGTTGAACAAGTATGGTCGTCCTTTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCAAAAAATTATGGTAGAGCGTGTTATGAGTGTCTACGCGGTGGATTGGAATTTTAACAAACA#9B^lr*8CQcs $/;GR^gs  +6@LYfu~+5AMXcp| ".:GR_lx !,7BMYfq(6CNXdp| "0;FQ]iv  # - : F Q \ h s     ' 2 > J W d n {   . : F R ^ j w    * 4 @ L W c n z   ! - 9 E P \ h s  '3?KWcny,8EP[ht(4@MYfr$1=IVbn{ !.9EQ^ju)4?KWco{ #0<GR_kx+8DNZfs'4@LXdq} $/;HT_lw +8DQ]it)5BMYfr} %2=IU`oy  +#9B^lr*8CQcs $/;GR^gs  +6@LYfu~+5AMXcp| ".:GR_lx !,7BMYfq(6CNXdp| "0;FQ]iv  # - : F Q \ h s     ' 2 > J W d n {   . : F R ^ j w    * 4 @ L W c n z   ! - 9 E P \ h s  '3?KWcny,8EP[ht(4@MYfr$1=IVbn{ !.9EQ^ju)4?KWco{ #0<GR_kx+8DNZfs'4@LXdq} $/;HT_lw +8DQ]it)5BMYfr} %2=IU`oy  + #3': %#4:66:1*+>/>>/>'>>>>11),;,+>>>>>; >>>;+>;>>;>>;>>>>;>>>>>>>>>>>>;>>;>>>>>>>>>>>>>6>>>>>>>>;6>>>>>>>>>;>>>>>>>;>>>>>>>>;>>>11>;>;>>>>>>>>>>6>>>>>>>>>>>;;>>;>>>;;>>>>>>>>>>>>;>>>>>>>>>>>>;;>>>>>;;>>>>>>>>>>>>>>>>>>>>>>>;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>888>88828>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>">>>>>>>>>66>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>::6::::::::::::6:::::::4::::  #/ #3': %#4:66:1*+>/>>/>'>>>>11),;,+>>>>>; >>>;+>;>>;>>;>>>>;>>>>>>>>>>>>;>>;>>>>>>>>>>>>>6>>>>>>>>;6>>>>>>>>>;>>>>>>>;>>>>>>>>;>>>11>;>;>>>>>>>>>>6>>>>>>>>>>>;;>>;>>>;;>>>>>>>>>>>>;>>>>>>>>>>>>;;>>>>>;;>>>>>>>>>>>>>>>>>>>>>>>;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>888>88828>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>">>>>>>>>>66>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>::6::::::::::::6:::::::4::::  #/"=K/O!'&d_=M[W8S_Q 9<*6dM_`;AE6)PB$R&STS UJMRX-)=8 KJM. 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"#  (N#'/   !3!"% %"6-J!#)-/%D(0:.3*$1wuy ">& ,AGGAGTGTTATATCAGTTGTGATAATAATATTTACACACATAACACATGGCCGAACACCCCAACCAATACTCCTATATCGACACCTCCATTCGAATCACTTCACCGTTCTTTGCTTCGTAAGGAGGAGGAAACGCCACAAGATCCGCATTATCCTCCTCAAAGACGAAGACGTTCGGGCACGCAGGAAGTCCCTACACTCGAGATCGACACGGAAGAATATTCTCTCACATCGCGGGCGGTGGAAAAGAAGCATAACGAGATATCTGAGATAGATCATCTTCAATACTTCCAAAAGGAGGATGGTCTGACTACTTTATGAACTCCTTTGGTGAAAAGGTAGAGGGTTAACTCCGCTTTCCTCTCTCTCGTAAAAAAGAATAACCAAGTACTTCTTAGAAATAAACGGGCTACCTTCGCAGTTTTTCTTTTTGGATTCGAGAAGGGAAAGAGGAGAAGCTGGAGGGCCGTCGTTCCAGGGCCAGGAATTCCAAGGTTTCGGGAGCAATAATATTCGCCGGGTAATATTAGAGAGCGTAATAAGAGTGTATTCTCCGCATCGTAGTAAGATCATTCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:46:26 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSWBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :uTDATA :ufDATA :u@DATA :uDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~VGLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS+TP1AM@BP1RL@=P1WD@FP2AM@OP2BA@@TP2RL@KPBAS@@-PBAS@@+PCON@@;GPCON@@9PDMFTPDMF+kPLOC@4PLOC@0PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%+LSCAN@SMED SMLtSMPL!!TSPACAkSPAC+dSPACAkSVER4.0SVER WSVER,,/Scal@Scan@TUBEH2Tmpr<UserphARphCH+phDYbigphQLcphTRphTR.This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSWBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :uTDATA :ufDATA :u@DATA :uDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabf+FVocfFWO_GATCFeat//eGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~VGLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS+TP1AM@BP1RL@=P1WD@FP2AM@OP2BA@@TP2RL@KPBAS@@-PBAS@@+PCON@@;GPCON@@9PDMFTPDMF+kPLOC@4PLOC@0PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%+LSCAN@SMED SMLtSMPL!!TSPACAkSPAC+dSPACAkSVER4.0SVER WSVER,,/Scal@Scan@TUBEH2Tmpr<UserphARphCH+phDYbigphQLcphTRphTR-Dq.$+W x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&rBA <3zfg\f$mGAM'/b[L:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]1S+.#ok M>@d+]'JwA^n\f: 6IkVVXs,Mn-k|cy[*oCfo;LY&0E(_y"%_oU@tC]Q#V>AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnP_I[Dd@2g|5J_@A[]"p64y^M_9ni|\Z&,Nl*]GWSTdXMPGg?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!3#C,F2id7-J!."^O[,z^E$6d+XnhcjPGh{;qeXd9UZf0DI$LzjyXY?D8dYb_Q{vRLUH}M>\oMiD$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUGB1Pt;B1Pt;BCTSWBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :uTDATA :ufDATA :u@DATA :uDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabf+FVocfFWO_GATCFeat//eGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~VGLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS+TP1AM@BP1RL@=P1WD@FP2AM@OP2BA@@TP2RL@KPBAS@@-PBAS@@+PCON@@;GPCON@@9PDMFTPDMF+kPLOC@4PLOC@0PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%+LSCAN@SMED SMLtSMPL!!TSPACAkSPAC+dSPACAkSVER4.0SVER WSVER,,/Scal@Scan@TUBEH2Tmpr<UserphARphCH+phDYbigphQLcphTRphTR