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(((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9329f9e6a3c541c883972815659f7720Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"########## 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'oY,L)CT/ZqChk:=yp(4e/"!!$(*'DFK8k1   'pZ+N3uQ&*k1tr4#%    /~p;U/^;    7Pc6Zu/        J2-Q        _]urUy.$b` 8k4  C"1jB    -rA>{:           /z_#>[; U.JE&f5 #&%    `2"oZ0!$!     -j$${qx$.g2 2xRE.dfysD!T./r^1 D B]X5R3$ IFR Bm[  MB    #VS4R*        VMfG_.  :mURy>>GYl;j^G9\J2zD'       DB1Q* C9rLut>   "TU:K+N0"  "L5Fb9$       "PB>b9"   ,bZq#S-  #S8}g#_8     .e f8_7   S {*I8@\:)" '9KZcc\PA3)"  G\ZK    !"!'5Mq4?I2bubXSNH@6,#   (4?EGC;0%   ^@qvAF ADAoKB.bcpKB_3730_POP7_BDTv3.mobtermAGAGTTATTGGGATTCAAGCCGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTTCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCAGTAGCCGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGCCGATGCTATGGCATCGAGCCTGTTCCTGGAGAAGAAAGTCAATTTATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATTCCTACTGCGTATGTTAAAACTTTCGACGGTCCGCCTCACGGTATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGTGGTGGACGGGGATTTTATTAAACAGAGTTATTGGGATTCAAGCCGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTTCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCAGTAGCCGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGCCGATGCTATGGCATCGAGCCTGTTCCTGGAGAAGAAAGTCAATTTATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATTCCTACTGCGTATGTTAAAACTTTCGACGGTCCGCCTCACGGTATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGTGGTGGACGGGGATTTTATTAAAC"6=Q_fq} &5BN[kx*5CMYdp}'3<HVcr{)3@KVbn{ !,8DQ]ky +6ALYeq(4BMXdp} "0;FQ]jv   # - 9 F Q \ i u    ' 3 ? 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Y  S]0E FHHd:"5K 2V&QAS.TQMT8DWPY )4_0!R4 D/:]%A;%# HC  bF50EG-R MOZ7B ].6 %204TH-&*P/&K'/6^A06I 9BZ Z$ ^`C% -%8(KB63JGI&MFI.RDI E:K!`0$TEE1 A (H--FTO4c/ P #[$5ZB+:-^MU5)! c2+5"MaU&.X H+"A\F=/14>V550KZ2UBT,Q?LU>EPWPKM:^[WWI&=*,/ _ P#IKEOHD,AGK _9KD 0ZHG`J5N%LHK" WA^B >A,$QO2"X2R^ /Cb+C1/,BRY,KXIKANU(?ab8=68["A!09" P*C$/?bN\^UZcQ4^-%?,!4_F%9P3 7bR]2ZB]<5/Y% -&Ra5[K")729'6< <F^dB;%/1$%`][b?0.d+8GQ4H6>6 P][3*U+K*-NQ<(RVQ:^&]: [4Y S3[R_QHacAj&w.+z7J}WBaQt,P O=-SOC0L_4*K"$)E -uGf C"Zp\ [fKyogL T_`xm``i<X987 4-Tb|n.Pl0cErji <EhX|tvbZ m HOu S]sB#$%.#F.b jDV|Wa)$wz:tW4=  Y$OuN ag"`'x0"#uFn"l9do*`,))K4jps  ~5/':05ls_N s=8}z@# d`_=(A4* 6Z )9az7 l ~K5h! qhBy%"t+_jQZnMXIJ>FC7@*A157 9(94 & &                        " & # "#'( + !)*"',4,5 #+").!#"-2H!u@~p,p,8,8wpqpDIppMrp +6),d P^ , }UNS#0pwQIWRaBo##v\[$Z BN; ;6^5$V:}tYP8^g}Ut <DR PMwp\-%amqpQRb8,Q>8!'fe -*-/EQG.q@G`]\ h-|D QoJXyUKbp??pOyO;GK7O}7'fJ6V3xUz(K~)pjd^  "/c2K!K7 mY~-@ ?w{a1=g(.^%> A--N6b !8h#(}5)$' "$&+90!#('U=*2$# 0?-"-3$(. $   4?B/"%.#%#!0!&9"+-)"%-/+-! 6'(A;&$+0)' ! %-+!.+  !8#$2+2!%&3("!&#'&-6','9)/6(1.6,&!%, ! 5 =2*.%*F6g |Y*"1.&  5#%,#,8F\-@**#!  ">"% $!5A>"C/(31")"-03& A$A'6/5*'<3>!8-Gk #qY(&f#d(-GAGAGGTGGTTATACTCGAAACTGTGATGATAATATGTAGCCACATAATACACTGCCGTATAACCCAACCAATACGAAGATATCGTTGGCGTCAATCGAAGGACTTCACCATCCAGTGCTTCTTAAGGAGGAAGAAACGACTCAAGCTTCGTCGTATCCTCCTCAAGGACGAAGGCTTCTGTGCAGGGCGGTTTTCCCTCGCTTCGTGGTCAACACGGAAGGAGACTCGGCTTTCTCGAGTCCGGTTGAAGAGGAGGCAAACGAAATAGTTCCTGTGGCGCCCTTTTCACATTTCACCAAGGAGGAAGGGCCGATTCCCATAAAACCTCCTTCGGAAGAAGTGGAGGGCGTATGCTCCGCGTTCCTCACTCTCGTCCTAAAGGAGAAAAAAGTACTTCCTGCTAAGGAAATCCCTAAATCGTCCGTTTTTCTTTCTGGATTCGTAGTGAGAGATATGGAAACCTCGAAGCCCCTCCTTTTGCGGCCATAAACTAGAAGCCTTAGGGAAATTTGGTATTCTATCCCCTAGTCTAGAGATCATAATGAGTGTGTCCTCCCTGAACTTTTATAAGATCATTCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:46:27 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSgBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <yTDATA <yPDATA <yGDATA <yhDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~f;LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS:P1AMDQP1RLDMOP1WDDV_P2AMD_oP2BADDcP2RLDZPBASDD=sPBASDD;/PCONDDK PCONDDHPDMFTPDMF;PLOCDD?PLOCD?PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%:SCAN@SMED SMLtSMPL!!TSPACAiSPAC; SPACAiSVER4.0SVER gSVER,,/Scal@Scan@TUBEG2Tmpr<UserphARphCH;*phDYbigphQLcphTRphTR4This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSgBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <yTDATA <yPDATA <yGDATA <yhDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabvFVocvFWO_GATCFeat//uGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~f;LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS:P1AMDQP1RLDMOP1WDDV_P2AMD_oP2BADDcP2RLDZPBASDD=sPBASDD;/PCONDDK PCONDDHPDMFTPDMF;PLOCDD?PLOCD?PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%:SCAN@SMED SMLtSMPL!!TSPACAiSPAC; SPACAiSVER4.0SVER gSVER,,/Scal@Scan@TUBEG2Tmpr<UserphARphCH;*phDYbigphQLcphTRphTRs)B23Hj.L3`|z&mm,_?DB~P^lQbaeX7SoR:+:j,$}<7-? |EXoW3Y~+ctPp%!++(aQQ*G]mysG(B-O*@NzRfu4WyYZ(Wa:nuXBT]qK_^_<~|Rs5AVih5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9rgzE<=#d1a-c_[O~}4}ceGf+Ko[!AETRF[+]18`F7IOoUVn"eHts'MP(D`~<)l47y!R}*D]d0oA{Qk@ /kXSE@a!)DS *n&-HOnO@5}"a'3.5s\T7}{W_z>FfM~V0Et5b'|2RV}ug4wT[%R7R>(hZ$~I!|spN$t.D~F45$\j|KA[Lz2fJq3IxdO#rT9fBQz?~|eF^@O!Ll~f[#6mOt:/`C?QjWgQ}QH8EE.Om,K]yDWYhY8|kN_uw >xPo1Cd}Fp82)XaM~VpYow.mU5s%<(]R06`NV-[?|$}~?V'i~?RVuUi\j-=p\q_W-'Ic6XH[AVePX!Hiwe2{sGiSVqrj/.3]HCi%{1k :O[O~}<"&eG1VcyST/4f_7}?e_MghW~^(';_/C]2ZE<}g6M>P:Y'e(nNc:p2kh16#\gzU_]tt/;k$;~6>udrVbH`6@v0kc V&Ip?H^Po)Gr(K.L-[DW[Zeh&0w#j'"LAX;:bz]QyQ]bxd$XF$~8+!3@*7_6SvrsPoIXz7_lnqR9vC.Vq`A #pJE?RVuUIcTA?i]8=D&)rj pT=3!\<]*:8-3_lEA_:k9vAMV^@=?]p&q6X+9mB\yE*<^k'eaK|`P@R(RlSR[7}?e=ES77~U?63_iP-2ZE<}|Uo%Z=r*r`sEY?bM:bFs5Iis|K/l3672tXMV^A$+vTE#!#+Xn$yYE!p;|WnT(%Fy1pLze~!Tq*nEg8Z~KI'_2B.*W"i-I7} W5WDt;x.qK|>VRJxG57'9\47.<<_