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  (((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0310fe60a01a41deb52e2b1342e7ff7aSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"########## 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T)[d/VB_g7k[6&"#!        ,pN0gc%I {fjc9% $fI<NiER{yK% !cMT-,k;%H9PJ(|N/ X)`rGw2I)te9  "Y p7U1   &_ .;c^5!  -i/ PfH'vC( L~Lyo1d {=mi3mf<%      8x7<Z5! 3msy+3.xyH*      +_`Tl96\jB+"  "K%sZ yH+    C+8Z4  AiEU6"      %Xa ;f?'  &Xj (@kE." ?|0gU8(    8|0|{1F   &AkDDzl/<~phaYPE:.#$1>JRWWQH;.! @$ AA?AAKB.bcpKB_3730_POP7_BDTv3.mobtermAGAGGTTTGGGATTAAAGCTGGTGTTAGGATTATAAATTGACTTACTACACCCCGGAGTACGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAGCTCGGGGTTCCGCCTGAAGAAGCAGGAGCTGCAGTAGCTGCGGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACCAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCCGTTCCTGGGGACCCAGATCAATATATCTGTTATGTAGCTTATCCATTAGACCTATTTGAAGAGGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAACGTATTTGGTTTCAAAGCCTTACGCGCTCTACGTTTGGAGGATCTACGAATTCCCCCTGCTTATGCAAAAACTTTCCAAGGTCCGCCTCACGGTATCCAAGTTGAAAGGGATAAGTTGAACAAGTACGGTCGTCCTTTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCAAAAAATTACGGTAGAGCGTGTTATGAGTGTCTACGCGGTGGACTTGGATTTTACCTACAAGAGGTTTGGGATTAAAGCTGGTGTTAGGATTATAAATTGACTTACTACACCCCGGAGTACGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAGCTCGGGGTTCCGCCTGAAGAAGCAGGAGCTGCAGTAGCTGCGGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACCAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCCGTTCCTGGGGACCCAGATCAATATATCTGTTATGTAGCTTATCCATTAGACCTATTTGAAGAGGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAACGTATTTGGTTTCAAAGCCTTACGCGCTCTACGTTTGGAGGATCTACGAATTCCCCCTGCTTATGCAAAAACTTTCCAAGGTCCGCCTCACGGTATCCAAGTTGAAAGGGATAAGTTGAACAAGTACGGTCGTCCTTTATTGGGATGTACTATTAAACCAAAATTGGGATTATCCGCAAAAAATTACGGTAGAGCGTGTTATGAGTGTCTACGCGGTGGACTTGGATTTTACCTACA)3?Waq+9DSds $/;GR^gs  *6?KWdq{(2>JU`my+7DP\iv(4?JVcp{ $1>IT_lx,7BMYfr    ) 5 A M X d o {   # . : F S ` j v    * 5 B N Y f s    % 0 < G S ^ j u    ) 5 A L X d o {  #/;GS_jv)5BMXdq} %1=HUan{ !.9FR_kw+6AN[gr~&2=IUalx !.:EP\iv)5BLXdp| %2>JVbn{ "-9FR]iu)6BO[gs~'3@KXdp| $1<HT_hoy')3?Waq+9DSds $/;GR^gs  *6?KWdq{(2>JU`my+7DP\iv(4?JVcp{ $1>IT_lx,7BMYfr    ) 5 A M X d o {   # . : F S ` j v    * 5 B N Y f s    % 0 < G S ^ j u    ) 5 A L X d o {  #/;GS_jv)5BMXdq} %1=HUan{ !.9FR_kw+6AN[gr~&2=IUalx !.:EP\iv)5BLXdp| %2>JVbn{ "-9FR]iu)6BO[gs~'3@KXdp| $1<HT_hoy'  %*: + '%2:46>1-+>6>>&+'!>>>>1>)/;>;>>>>>;.>>>;1>;>>;>>;>>>>;>;>>>>>>>>>>;>>;>>>>>>;>>>>>>>>>>>>>>>;;66>>>>>>>;>>>>>>>;>;>>>>>>>>>>>116>;>>>>>>>>>>;>>>>>>>>>>>;;>>;>>>;;>>>>>>>>>>>>;>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6;>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>16>>>>8>>>>>>>>>>>>>>>>>>>>>.>>>>>>63>>>>>828%88838>>>>>>>>>>>>>>>>>>1*>>>>>>>>>>>>>>>>>>>>>>.>>>>>>>>>6.>>>>>>>::::::6::::::1116:::::6:::::::6::::::66:4:::::::6::::::::::2:::::"  2"1   %*: + '%2:46>1-+>6>>&+'!>>>>1>)/;>;>>>>>;.>>>;1>;>>;>>;>>>>;>;>>>>>>>>>>;>>;>>>>>>;>>>>>>>>>>>>>>>;;66>>>>>>>;>>>>>>>;>;>>>>>>>>>>>116>;>>>>>>>>>>;>>>>>>>>>>>;;>>;>>>;;>>>>>>>>>>>>;>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6;>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>16>>>>8>>>>>>>>>>>>>>>>>>>>>.>>>>>>63>>>>>828%88838>>>>>>>>>>>>>>>>>>1*>>>>>>>>>>>>>>>>>>>>>>.>>>>>>>>>6.>>>>>>>::::::6::::::1116:::::6:::::::6::::::66:4:::::::6::::::::::2:::::"  2"1 7KGE*AIC84 [6;:@NUO]%:H 8/cYDZ %#CM K99^B'B7?EQ->(IJ*WRZEI JU\O8KZ%T@:W')#3D)^PDP^SN)MZ1DT#!V:(40GbE,>] 06/ ^@ A]` c"UIF&L$F2FD=&H51Y8,9 @#22% L9QUd+3`O  W@["3 @bKJ<':7#0/b9EKSc63>TM9 R!` .P2'Z 1- A-+(BHA:L FJ+\UN %Z>L Pb2:<P2&&Ja,E&8-H+FMOL0H8&IXI(5:*8#H L  X'?> RV!A,X836-D#c8X5K"<@"JN]+(NE2\CF6AIS@/OGJT<b"&<caT>>>;G.5;DX <)&=G EPb VN&Q?S,Q0 7?_[[SX O 8K./$LE 0X5\$6;,0YP5Mc,Sc$6\JF. 2K%HKNG_B#>(Y`P'C,J JJ;3I?;/5S4KT]C2$.!cD $ -:N;2N +R`) ]McHEX:] '!D]b<NP Gcne\:y;j-OR,01YZX3-;d=!+9*ZuYK!!7>1QjF{ A6 h: j3m4.]W]Wc@yk G,4mcn*H-fCOLO)1d{ +j*x\|OB-wdf(YUH 3D 5 Z@; SuO7S.'?VI". ;RJ8I\xwZ#qKvcqkC<1|=((]ha~P|.kR. `L\=M!m\#(\y_!`DV=[r :Lk\0"n2NB#O~u R3y#s<wlW6P7kUe't#RMehLw}X?P/3 i Th0}rw6} TXrx>"JP GA  -}Y^f[LIKXA?23&( 0:7)E,4>                          )# '  ,")"#).%"' '#!!+*)0 !$'$)"#1.(4#8.-,!$/((-7)HR57%9%8()-9(04*4/(?(9!6*&%)''  %%  spkpp,^D5mgjd~8,L,[g\0pO !#e0p!]IMwd8p[o8BQvA)PRGd3CZ \QMjSN8d,<v,q{d_K+tmjHd8R'~40GN/OM$s+Ak '&98wd8CVHdo 4!0\t s- Gdd,8Ww5jop<1|%8td{8Ep#dprE%^ 'aq}zXMBv4 @ W\<BA 5S vz+0F&KBu<fq#f0!28p"<4'(    )9' # %*#$#'+ " .&(0(( $  !8%(()' $!*'!"&")*")$(,&%2 ( %""%!$5$  $(%( ! 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"0$''0-G!&&!"0 ('0$$!#%  #&*23@?v~F5!'&;$ "*A  !$'4BN - )%*+'$) 6`!&4): ):$-#!,3!(B '64#&"*5+4B,8*-9)0({#9.GAGAAGGGTAATAATTGCGGTTGTGATAATAATATTTACACACATAACACATGGCCGAACACCCCAACCGATAGTCCTATATCGACACCTTACTGAGAATCACTTCTCCGCACCATGCTTCGAAAGGAGGAGCAAGAGCCGCAAGCGCCGCAGTATCCTCGTGAAACACAAAGACGTCTGGGCAGACAGACACACCCTACAATCGAGATCCACACGGAAGAATAATCTCTTTCATCGCGTGCGGTTGAAAAGAAACAGAACGATATATGTGAGACAGAGCATCTTCAATACTTAGAAGAGGAGAATGGTCCGACAAGGACAAGACCTCCTTCAGAGAAAATGAAAGAGGTTAACTCCCATTCCCTCTCTCTCTTAAAGAAGAAGAACCTAGTACTTTTTCATAATAAACGGCCTACCTACGACGTTTTTGTTTCCGTATTCGAGAAGGGAAATATGAGAAGGACGAGGTCCGTCGTTCCATAGTCAGAAATTATAAGGGTTCGGTAGTTTTAATATTCGCCCGGTAATACTAGAGAGCGTCATAAGAGTGTCTTCTCCGAACTCGTATAAGACAAATACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:46:26 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt6ASPt6B1Pt5B1Pt5BCTS\aBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;\vTDATA ;\v|DATA ;\vB4DATA ;\vDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ZLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS/P1AMCF}P1RLCAP1WDCKP2AMCTP2BACCXP2RLCOPBASCC2"PBASCC/PCONCC?PCONCC=qPDMFTPDMF/PLOCC8PLOCC4ePSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%/SCAN@SMED SMLtSMPL!!TSPACAkSPAC/SPACAkSVER4.0SVER \VSVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCH/phDYbigphQLcphTRphTR5This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt6ASPt6B1Pt5B1Pt5BCTS\aBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;\vTDATA ;\v|DATA ;\vB4DATA ;\vDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabjFVocjFWO_GATCFeat//j|GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ZLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS/P1AMCF}P1RLCAP1WDCKP2AMCTP2BACCXP2RLCOPBASCC2"PBASCC/PCONCC?PCONCC=qPDMFTPDMF/PLOCC8PLOCC4ePSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%/SCAN@SMED SMLtSMPL!!TSPACAkSPAC/SPACAkSVER4.0SVER \VSVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCH/phDYbigphQLcphTRphTRf\mci2aqN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`Aq>:YINNfpNRW8dosa[lV]r.*x_[c|_nvC]_y!iU5|8e7sINi@C,_,qz7z}yaih?]R_s_bed{a9_ajFO-U[dUDNxGq@p'Ic62nrD5pY^d,/IQ}{sGitG3(|}jGPNYQIxw}H{-*<^k8e7?GOV5ro{`qafE*&aF2`_c&a6';_szT/"a/hV6T]==6X*s$[-T_sKQm5APYXA\95Lq>l>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdO#rT9fBQ~?~|eF^@O!Ll~f[#6mOt:/`C?QjWVQ}QH8EE.Om,K]yDWYhY8|kN_uw >xPo1Cd}Fp82)XaM~VpYow.mU5s%<(]R06`NV-[?|$}~?V'i~?RVuUi\j-=p\q_W-'Ic6XH[AVePX!Hiwe2{sGiSVqrj/.3]HCi%{1k :O[O~}<"&eG1VcyST/4f_7}?e_M%_|_n?\h =&e=1&!rE_g4YDI#%+AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt6ASPt6AUDTxB1Pt5B1Pt5BCTS\aBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;\vTDATA ;\v|DATA ;\vB4DATA ;\vDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabjFVocjFWO_GATCFeat//j|GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ZLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS/P1AMCF}P1RLCAP1WDCKP2AMCTP2BACCXP2RLCOPBASCC2"PBASCC/PCONCC?PCONCC=qPDMFTPDMF/PLOCC8PLOCC4ePSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%/SCAN@SMED SMLtSMPL!!TSPACAkSPAC/SPACAkSVER4.0SVER \VSVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCH/phDYbigphQLcphTRphTR