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(((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a2b067e9bd8f408190abe5508e09bef5Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"########## 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KG8<`D_G7"7+7OC"<@VP;2cCNLa,72*PR&O@8" "G6cUD. O9L-K%$M B:V2ED;!T >`Wca:= Ea-OW9JE">_*M2>\(a[K+c+=^X2=EU;4:09N-JD6cGIS& T> .;[9=-("2cZR< 0 Q)L9I'[*R"]-.81cK> #G7>[VaXJ2!=C[?O6G(6?RL,03)>CW_7_A00 U+]<5`+"b Y0ggs+#-!n`/gMu4  UfOcE2kgNn1q5-<b}$n%SeAhy=DN:?d %[vu][FezoJrW/TE k2/ot|FI%_J;Gp _.)h[?ChYCJAGFCo'L/@)S!`5J@lF$~D/!2m=}n" ~KOQ0,8Z*- fRv;'ZimcE,*)$,rlzfK&`^FulpcNFbG$:X14Ot5j/-A/_<c9!\AYYHFm/5 _6V "(8QI2lh HH H v]|:J)Z |qXWkSSKJGG=>@3>62;*5-_)")%0# "             %)     !& %!''!! /%!2$!!%"$&9 6**'%%>&-:-''76938'())+,169%0*@&#4*%#'#$!!%%%#%O]&pGV$wpwdFptp2!5 p?p]X8Z^pplp %RdCm*W+gYbg[#pUp,8>3<o"Ow d7Kdp>"CfZ?/C3K,bp6lC=,"pO\;8s#q>NQSC\k|"h&pPO5dR`2[LBR ^s#5!81XmpyX*UHO[kIm'=/dD[ymrHQZ8n+qD{p'^p#,UuxjJR"HPL8@\Q/'? CRr3CO<7G:4:D$!k\- $#c&#X10'"%! ! $+"'&3 I6#/6!%X:%5 &' :"3$..+C0*>!*)   # !$".&,6A&'(%'0 $ /-&=">!04+%$,=: H%3*('!#*% /&."+)%"4&#0.&6%2#(502,)!"D#!4 (-@M+"3!F3= *2)844 +&""8. (&&# (2$&8'$:'1*^9tR++ 0!!3&% -*#" #'(;./35ID0,),")K!,=& (+*'7A4!4,-  B40$"#<-2!,G/C+/(]*/+6-C>*.h*_V@CSy:YAAGAATGGGTTATCTCTTTACAATGTGATGATAATATTTACACACATAATATACTTCCGGATACGGCAACCAATACACCTGTATCGTTGCCGTCCCTCGAAGCACTTCTCCATCCAGTGCTTCGTCAGGAGGAACAATCGGCGCAAGATCCGGCGTATCCTCCTCTTGCTCGAAGACCTCTGTGTAGGGCGATAGACCCGCGATTCGTGATCAACACGGAAGGCGACTCGCCAGTATCGAGTCCGCTTCCAGAGGAGACTAACGATATAGCTCCTGTAGCTCCCTTTCCATAGTTCCCCAAGGAGGAAGGTCCGATTCCCACGAAACCTCCTTCGGTAGAAAGGGTGTACGTAAGCTCGGCTTTCCGCGCTCTCCTCCTGAAGGAGAAAAGAGCATTTCCTGCGCAGGAGATTGCTGCATCGAAAGGTCTTCTTTCCGGATTCGTGGAGAGAGATATTTAAAGCACGAGGGCCGTCCTTTTCCGCCCCGAAACAATAATCCATACGTAGATTTGATATTCTATCCCCAATACTAGAGAGCCTAATAAGTATGTCCTCCCTGAACCGTTGTAAAACAATACABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:46:44 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\ DATA ?\S|DATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR<P1AMEi!P1RLEdP1WDEmP2AMEvP2BAEE{IP2RLEr5PBASEETPBASEERoPCONEEbRPCONEE` PDMFTPDMFRSPLOCE[PLOCEVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R4SCAN@SMED SMLtSMPL!!TSPACAe͚SPACRLSPACAe͚SVER4.0SVER  SVER,,/Scal@Scan@TUBEF4Tmpr<UserphARphCHRjphDYbigphQLcphTRphTR4This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\ DATA ?\S|DATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabrFVocaFWO_GATCFeat//2GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR<P1AMEi!P1RLEdP1WDEmP2AMEvP2BAEE{IP2RLEr5PBASEETPBASEERoPCONEEbRPCONEE` PDMFTPDMFRSPLOCE[PLOCEVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R4SCAN@SMED SMLtSMPL!!TSPACAe͚SPACRLSPACAe͚SVER4.0SVER  SVER,,/Scal@Scan@TUBEF4Tmpr<UserphARphCHRjphDYbigphQLcphTRphTR/o'zbMaQd&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23i7FbXtrW+b|=e#=p%EZJIB_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZ~0^Cu4Yy4_^ZBFf?0p=P#`0UbpE%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa-s1tob*oCfvn}4|Md'ac_` WV|N&4MM~rMnw]xk,C`WRk.e{:^kkSOj!Q4KK*flVy8J9tGHrd+"L&d&2<\d&k@6GZ]]C)TiSOpP S!oS{Rn[-Vt2`\wmj7ar?}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47./D?*&qDp-o*Cwh1nk-~4Kb}wLD7X{Mf4-gAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\ DATA ?\S|DATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabrFVocaFWO_GATCFeat//2GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR<P1AMEi!P1RLEdP1WDEmP2AMEvP2BAEE{IP2RLEr5PBASEETPBASEERoPCONEEbRPCONEE` PDMFTPDMFRSPLOCE[PLOCEVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R4SCAN@SMED SMLtSMPL!!TSPACAe͚SPACRLSPACAe͚SVER4.0SVER  SVER,,/Scal@Scan@TUBEF4Tmpr<UserphARphCHRjphDYbigphQLcphTRphTR