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  (((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ab5a6aa674044241be75e296fb7b66e2Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"########## 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I9]9*K_Q DO$`b/OIU64PY P +A^R>/0W)@ YcCJI F(!:UH^  K/B(Xc0  %(ZB\A?a4Na`^*WRF*L,:TN&6E? ,X/`>`Jc"ORS  $T[N0R>XK0VNLUR`I7 IM[6bNMKYVF !/"=,/IE)R_!%H[>)DEaD7&^C(U0Z 4%0XD"4 3P RH!"^ Q75^0S5><+ A]".=5 ;XW&T T&@a#+&Y/4$)O:UC*97@TO /)/+,U0Z25F_Z+I.Y(_4 3a U?Z.\WC&,<E%BRT*a # ,M7d$W#BG\1W( >NT[74*^=VG9 ,5L\A$Q@$_;P +*c:&BO!\^ &  /; 5:+N NUK 5!W@2"HKEB.O2<$-1 >LK!8)[* J_ ^/` 8]AJR-8-T07PL[7@ZL4='2C CZP%c3+^F@_!47A;0EFK))0>$5U-:QSRN)(]0>2 1&P"d9Pj)Io-su 8U-*n;aB2Ub5e4'HUOd(  < L$Lsjb_1#+{&@fY{]O g8ld<N~R*o^akn1IFKdw8og (mp$8 EAy[Eq>$kke$rah8kf^$ K]"1}C_N z6/[O5#(vK+([.vuh 'fC;z uu]}O $AW9 l_' {=|BY A l?a*/3sfPS[:m t t*R4ZBV5ys|G]egz g<5FJGP Z & N\"un^pobii.@ b}vgC Mwc h,\ $Z.$w=[XXaQGNLG>7I492/0+"-*                &      #$+D) #$ #+"'$ %(+$168'!%C&$"&-;,!3*,5$0%3(525'.:,<),  #ppppxpp?Dv8ZyQp=,- ,8pgD q8'YluTq@&Gf+aNdlSpErph{|@ 4`ibcd_WOB7c?'p^BpXs^;+}FJb(-D/UN 8km6>i896=O$pd=9Vn;#,08!}L c  5%ZU<#kpcsHp`LASO2)bVi4Wp,R2%#g.pT[p: 21VS >Vs<3QIe*E]L2AORBLD,29%\3" +)QGm @    #!&!*>7) -B#5#50'0"/'C8#7)1!!( #"'5") *"7!!%*&!)14,'% "#$@     $' $8 '/" *! 0&-,- + & . & ' !&&(->/&1*)!*-)"0+6&3%+""!'"$  ")-'.*8)Yt%*/'  '    !#(56<S2"*12  0& 'FB9() !  -%#/?1(*%;%E'!?">2/Q@BB0&W+.lj_d"GAAATTTGGTATATTCAGTTGTGATGATAATACTTAGACACATAATATACTTCCCAATAAGCCAACCGATATTGAGGTATCGTCGCCGTCCTTCGAAGTACTTCTCCAGCAAGTGCTTCTTCAGGAGGAAGAACCGGCGCTAGCTCCTCATTCTCCTCCTGTTACTGGAAGACGAGAGTGTAGAGAGGAAGACCCTCGCTTCGTTGTCGACACGGAACGAGAATCCTTCTCCTCGCGTCTGGTGGTAGAGAAGGCCAACCATATAGTTCATGAAGCTCCTCTTCCAGACTTCAAAAAGGAGGAAGGTGTGATTCGGATATAAACTCCTTTGGTGCAAGGGGAGGGGGTTTGCTTCGCTTTCCGCGCTCTCCTCCTGAAGGATATACAAGCATTTGCAGCTCAGGAAATGGTTAGCTTCGAAGGTTTTGTTGTTGGATTCGTGGAGGGAGTGATTTAAAGCACGAGGACCGTCCTTTTGCGGCCATAAATTATAAGGGTTAGGGAGACTTGGTATTCTATCCGCAAGACAAGAGAGGTGAATAAGTATGTCTTCTCTGAACCGTAGTAAAACACATCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:46:31 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1Pt B1Pt BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\ DATA ?\S|DATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}ULIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR<P1AMBiP1RLBdP1WDBmP2AMBvP2BABB{P2RLBr PBASBBTPBASBBRoPCONBBb=PCONBB_PDMFTPDMFRSPLOCB[wPLOCBVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R4SCAN@SMED SMLtSMPL!!TSPACAgt:SPACRLSPACAgt:SVER4.0SVER ~SVER,,/Scal@Scan@TUBEE2Tmpr<UserphARphCHRjphDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1Pt B1Pt BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\ DATA ?\S|DATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab9FVoc(FWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}ULIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR<P1AMBiP1RLBdP1WDBmP2AMBvP2BABB{P2RLBr PBASBBTPBASBBRoPCONBBb=PCONBB_PDMFTPDMFRSPLOCB[wPLOCBVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R4SCAN@SMED SMLtSMPL!!TSPACAgt:SPACRLSPACAgt:SVER4.0SVER ~SVER,,/Scal@Scan@TUBEE2Tmpr<UserphARphCHRjphDYbigphQLcphTRphTRV"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49WW!'YA EuzrH87$z~% VL\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUhM'k4+M2}AlVqCjrPCgBCQnl(}wp9Qtu49"c.VDTj.uFr~o[|!Y<,h`%mI{Mb=KubQ2a5qO Jm}Pz;+hj3ST?0sKG'BNp3<("Hf.Q F3eG"k@j/.3]J&sCe#&\7`[qDr_WG\|J_j.u%xfmFoM/V,<{1z^{5te.ER]QZEpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72Wei@8/(8\_pzN02%B4O7yr[q?Ib\fV9u,#03]/qf[ZL6`8dBw'JQEhXBN4<UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4