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  (((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 771f0ece514b4c1eba4b5a2f826b3d07Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"##########K1321_D3_Begonia_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z$V>vb$rNG 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Aw [Op@"     :e<HE0\8$       (EuXNLx%\<+%$" "6[do.lB'           %@k6yP W6! +Q9grF ^:#         !:^OJU6$3X+}8zP3!#<e6J{M/      %P$il'P%      !%/Eh63kb&-oaWND:0)"                             @_AA>bNA)KB.bcpKB_3730_POP7_BDTv3.mobtermTAGTTTTCATGAACAGCTAGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATCAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAAGTCCTCAACCGGGAGTTCCACCTGAGGAAGCCGGGGCAGCTGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTATTACATCGAGCCTGTTGCTGGAGAAGAAAATCAATATATTGCTTATGTAGCTTATCCCTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGGTTCAAGGCCCTGCGCGCTCTACGTCTGGAGGATTTGCGAATCCCTACTGCTTATGTTAAAACTTTTCAAGGCCCTCCTCATGGTATCCAGGTTGAGAGAGATAAATTGAACAAGTATGGCCGCCCCCTATTGGGATGTACTATTAAACCGAAATTGGGATTATCCGCTAAGAATTACGGTAGAGCTGTTTATGAATGTCTACGCGGTGGACTGGATTATAACAATATAGTTTTCATGAACAGCTAGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATCAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAAGTCCTCAACCGGGAGTTCCACCTGAGGAAGCCGGGGCAGCTGTAGCTGCTGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTATTACATCGAGCCTGTTGCTGGAGAAGAAAATCAATATATTGCTTATGTAGCTTATCCCTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGGTTCAAGGCCCTGCGCGCTCTACGTCTGGAGGATTTGCGAATCCCTACTGCTTATGTTAAAACTTTTCAAGGCCCTCCTCATGGTATCCAGGTTGAGAGAGATAAATTGAACAAGTATGGCCGCCCCCTATTGGGATGTACTATTAAACCGAAATTGGGATTATCCGCTAAGAATTACGGTAGAGCTGTTTATGAATGTCTACGCGGTGGACTGGATTATAACAATA $8NR`isx!.;FU`r~ $/:HS^iu(4>JVcpy'2>IT_ly*5AN\hv'2=IVcp{ %1>IT_lx,6AMXeq    ) 6 B M X d o {   " - 9 F R _ i v    ) 4 A M Y e q ~  " , 8 C O [ g r ~   & 2 = H U a l y   ,8CO[fr} $0=HS`lx ,8CP\iv*7CO\gt'3?LXdo{ #/:FR^iv+7BMYfr~'3?JUbmy "/;GS`kx+7DO[gs(4ANYfr~ &2?KWdp{ #/;GS_hp # $8NR`isx!.;FU`r~ $/:HS^iu(4>JVcpy'2>IT_ly*5AN\hv'2=IVcp{ %1>IT_lx,6AMXeq    ) 6 B M X d o {   " - 9 F R _ i v    ) 4 A M Y e q ~  " , 8 C O [ g r ~   & 2 = H U a l y   ,8CO[fr} $0=HS`lx ,8CP\iv*7CO\gt'3?LXdo{ #/:FR^iv+7BMYfr~'3?JUbmy "/;GS`kx+7DO[gs(4ANYfr~ &2?KWdp{ #/;GS_hp #  2' '$:*46*)*>/4//88>>>>>>>/888/,%>>>;/>>>;,>>>>;>>;>>>>6>>>>>>>>>>>>;>>>>>>6>>>>>>>>;;>>>>>>>>>;>>;>>>>>611>>>>>>>>>>>>>>>;>>>1116>>>>>>>>>>>>;>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>;;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>;;>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>8>>>>>>>>8>>>>>>>>1,>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>>/>>>8>88.88/+>>>>>>>6>>>>>>>>>>:6*::::::::6::6:::::::6::::)6::::::6'::::::::)/:::3::5:::::3)3::::3::::2:,)3:::::6.:::::::36::::::6:6555:::462    2' '$:*46*)*>/4//88>>>>>>>/888/,%>>>;/>>>;,>>>>;>>;>>>>6>>>>>>>>>>>>;>>>>>>6>>>>>>>>;;>>>>>>>>>;>>;>>>>>611>>>>>>>>>>>>>>>;>>>1116>>>>>>>>>>>>;>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>;;>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>;;>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>8>>>>>>>>8>>>>>>>>1,>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>>/>>>8>88.88/+>>>>>>>6>>>>>>>>>>:6*::::::::6::6:::::::6::::)6::::::6'::::::::)/:::3::5:::::3)3::::3::::2:,)3:::::6.:::::::36::::::6:6555:::462  1?)W;5Uc-63YaaQ# Q+>@*]=88,;cR?>cJSb+HP>-5b= ^X<7\*H<@cE4XBEcB(bXNMK(,'J]`L<="YT3VY4Y5A>>8>Q/>NA  W%MS06LMGG1H*`>+C"&2-`$ >!4&:6%)Z RJ +EKTPGFY:?!$C#@F1D?-B!aISR, ZT8^SX?IJOWKE:*[.H7(E.E43 A2V )I0L4P)%/ 4`J,HR=+CUN(:9bb:7O32C 34.F1 R? 7]P4c\=DZQS1[ 3?Ub?F*,A FD MJA1(53;@K!"F;CF33U -=067#:dJ_R$['40-21CI .I%7\BYdF' ^ :91D#=OKB*]#b_C4&*A5%_;%,V>5c3`Y_J7X Bb,`XF%HVS` 7'<']]H25-5TJGZ'PL'K3T ) NOH4A3_-ALTAZ$B1 1@MH,J29_]QU?@&Q4V(2FQ);#Rm %lpL1 }SOO' {39.Tf VJ8#3)U+t.u-9};8 yr!Fxh@.s)(C]KR-Uh$z 20PI 3  viAqD\&na sg2ExfuhNJavW B %%#;:rfH.O~xi],} [d2=APjbyw9 iMPM7X|oy(%9iI?vY,t-&8DOjo6bJr"vjW^{;.T~Sf.}D&eP!f;*?zuUQk=.572RSB-`S#ms. wF:b9B3eY]([Y-n'  u<[M+Q0F XK jyliQ(llwYeoRX_m]aSQIR.NMSI9NN8/,A1(/$%  $*&&                     $ ! !  "$#   +#"$, 7615&/!'&-, !"$/!"#'"0'!(0)$$A&56/.?>($$5 35/5'8G<:)297:4:4NAf3)1-;>62;>^UBY?AFJ?OEBBL<:FDHOWWC<ZQDMOPB[MFV\EIcYWpPOHH1:???::?:????gp,\W,p,,p.P p_>gp1/&p>EMg%d:,S%~`3~R,ddd}w S0WgE d?&Wr\cp1odIWl!dO*yU$`I BOf?xkqDO"y,,pD"I,oS!AQl},,gzur5upU,CQ8?8q^4/C ,~&h wQ udQApvpppW*(("(8:ACTU/5-'#&U`'=88E'!!+:(@C".Ba*3+1K6:: .,C9=.&.(C+3,+^IK$27MIU2,^9A@,7K2z`"Jz)GGAGGGGAGGTGTTTTAAGAGTGATGATAATATTTAGACACATAATACTCGTCCGTATAACCCAACCGATAGAGCGATATCGTCGCCGTCCTTCGAATCCCTTCACCGTCTAGTGCTTCTTCAGAAGGAGACCTCGCCGGTAGCTCCTCCTTATCCTCCTGATACACGAACACCTGTGTGTACAGCGGTAATCCCTACCCTCCTTCTCCACAGGGAAGGATACTCCCCTATCTCGCGTGTGGCTGTAGAGGAGGGTAAGGATATAGTTGAGGTAGCTCCCCTTTCATACTTCCCCAAGGAGGAAGGGGTAACAAGCTGGTAACCTCCTTTGGAGAAAATGGAATGGGCATGCACGCCTTTCCTCTCTCTCCTCCGGAAGAATAAGCAAGCCTTTCCTCCTCAGGAAATTCTTACCCTCGCCTTTCTTCTTGCAGTCTTCTTGAAGAGAGATATTTAAACCACGAAGGCCTTCTTTTTCCCGCCATATACTATAATCCATAGTTAGCTTTAATCTTCTACGACTAACTCTAGAGAGGTGAATAAGTATGTACACGCTGTTCTATATAATACCAGCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:46:47 -04:00AEPt@xAEPt@xAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?ZDATA ?ZSrDATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}!LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR.P1AM@hP1RL@daP1WD@maP2AM@vaP2BA@@zP2RL@qPBAS@@TPBAS@@RaPCON@@b!PCON@@_PDMFTPDMFREPLOC@[aPLOC@VPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R&SCAN@SMED SMLtSMPLTSPACAfxtSPACR>SPACAfxtSVER4.0SVER ~SVER,,/Scal@Scan@TUBED3Tmpr<UserphARphCHR\phDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt@xAEPt@xAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?ZDATA ?ZSrDATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~}!LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR.P1AM@hP1RL@daP1WD@maP2AM@vaP2BA@@zP2RL@qPBAS@@TPBAS@@RaPCON@@b!PCON@@_PDMFTPDMFREPLOC@[aPLOC@VPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R&SCAN@SMED SMLtSMPLTSPACAfxtSPACR>SPACAfxtSVER4.0SVER ~SVER,,/Scal@Scan@TUBED3Tmpr<UserphARphCHR\phDYbigphQLcphTRphTReih4qH4|t2#OoV$ zrTbZ?a23|jyHAj}W2yv!)QBmO7]N=]TG4/CeG1{?{sb: h,`,l6IEUY[^wA[h@t#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`K\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nvP?QYMA67E |VzM:*]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&'n[Nn%9SXWl573!dX!%~AfzvrfzoB=SLgu 6MjHbinOY5_}4]'>'s1HTf{fzja:3AG}X`=/r.ZNum#0kc "7:I12gbXW,62Q$OEHbX\"BV_@iD]xy"^O[,z^E$6d+XnhcjPGh{;qeXd9UZf0AW$rMWNK6EC2Ow7fwYIs=A&[sKFMrRU)>`Vn!<|'kT'_C:[rn{.QHT/RD8z{KJ[.z^`A #pJEK^;S4fT{-*<^k'eaK|`Q@#=,`Pf)NKo-Z&@GAI1_]GhnWe^kQtAcP)>`#6E1_@dUa_eM~|[{0%4dc{-m'i2}oGXS:.\_'[wA#Q9?kJ:h6Z]}4nXL-`S_YT{jqT7`h?kXM%YVJ|Q}.z zFTK(>V&j"SD=y!.qK|zW,DK >x(Y7+rIel1}ssb[l"G>z x:H-p@vdkel{F&+?cfoQe=N^tpuze/%{M*AqbEdG9} P,hz9_"#kjFO-U[ds=:I1On(I"]rr;j>VN|aVY,tIZ|]);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ilS-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03SSPACAfxtSVER4.0SVER ~SVER,,/Scal@Scan@TUBED3Tmpr<UserphARphCHR\phDYbigphQLcphTRphTR