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  (((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7e6cbed4421848ce885cd36b47fb4187Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"########## 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A?+2/6+IG9F!LIS3VRN3_ITS?L3B5WII:='91a5@RR*c"/M$R/P:;?B[ AS$"_Q""[S\[5]1N A;%\&+!"M8NJ@GHT9^YX#YYZT0 4]%6)T3,!(H'D5$ % a8,Z\\!R<GY/Yb?P&W"'cSZV5[)YIT.U:Q; >C'#PF!R(N`\] :O)4 !a5EI*=466^U^14:ZZ !Z5* "H4*9$[JMSK*MO!05G@8&7<%N4 ?TEK )F; 5\GG! (6" dJM&7 ?;P#153!N7=CIR\$DI7K[T8+5 KJ,7@S^;M0=[ :LR?V5@$:HY ^0CEW7S2X +6Q52%8T H #1 pGZtq07i>;|8t8Fx DwT,^'v>?SQC)ftYgYt=tYWq'J~ hIA\vT)IK ?tg{rDvhcH?:Ifg? @m9A6H@l'tFxOv\xY(7RE_C:0[+(+3sx Nev2,$O IJLvVn-z;\c7 =uv>d^u!P MH]K@Puuo"c $;3JbpdC)b"~usxy1tK0hZEs,gw"} V3PLe_ IF L $Q #WL(~bY&( s { eH|T|M!/<  saVkX[RJSH=;C3=:87%395-"(#                 #*     .  ! $''!("1$%"'%.08(#(0 &5;)#>N*;1 0"!,*6#&#*$?3@,$#        ARJ_%m[ppXBLq.~L Ppg?b0d-f,zobm JupSyg "2Cp)GV"QO/%5XcKtdnp3NE0vv \a MNG9>7K5){8H#=WSn "Jdmy'w7>jze|6V?f&1>sPS~cJQrFd np^q+l;p .7[p#T7Mp![,U{i;&!s^pr5&udc3Tk"XZw51np0]TKT *{s7FP1D<45c58"*j4E5#- Iz*'G.$BE% *A,:+$:'?1)="$L I98#:#20(LjACN*%24+!=/7#BH>";"!: 00! - '# "54G"#)*'"#! )-$ ) $'$%2"%#5&$!1,++$8;!)*0"$(+%!  "(-$) +'7!&*'!.@+!3& &1!*5-$"*%.(>H;4C5"%$(0##!&.%)!8$,78" *0$,'(:'!,*'!4/'+D9% ":8&9-#%#63&*  J'&!*&()()-KukQ=##"&%%-*##.) *"LT85>K3#'((m % /"**#$&)&(!/53<87 ?=+27?+#("%D+X%2"@%''7)6<+0/;rv9> Ft #1JGGGATTGTTATATCGAGGTTGTGATGATAATATTTAGCCACATAATACTCAGCAGTATAAGCCAACGAATACGGATATCTGGCCGCCTTCCCTAGAACCTCTTCACCAGGCAGTCCTGCGGAAGGAGGAAGAACCGGCGGAACCTCCGAAGTATCCTCCGGTTGCTGGAAGACTTGTGGGAAGGGCGGTAGTCCCACGCTTCATGGTTACCACGGAAGGAGACTCCCCAGTAACCCTGCCCCTTGCAGAGGAGACCAACGAGATAGCTAAGGAAGCTACCTTTTAAGCGTTCCCAAAGGAGGAAGGTGTAATTACCTGGTAACCTCCTTTGGAGGAAAGGGAGTAGGTTTAATCGTCTTTCCTCCCTCTCCTCCGGAAGGATAAAAAAGCCTTTCCGGCTAAGGAAAGCCTTGCCTCGGCCGGTCTTCTTTCAGGATTCGTAAAGAGCGATATTTAAACCACGAGGCCCCTCCTTTTGCGGCCAGAACCTATAACCCTTAGGGGGATTTACTATTTTACCCCTAATTCTAGAGAGCGAAATAAGTATGTCCGCCCTGAACCGTGTTAAAACAATCCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:47:05 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?^TDATA ?^"DATA ?^SDATA ?^DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE,LAST~~}JLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRDP1AMAiP1RLAdP1WDAmP2AMAvP2BAAA{ P2RLArPBASAATPBASAARwPCONAAb>PCONAA_PDMFTPDMFR[PLOCA[{PLOCAVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R<SCAN@SMED SMLtSMPL!!TSPACAfSPACRTSPACAfSVER4.0SVER ~SVER,,/Scal@Scan@TUBEC6Tmpr<UserphARphCHRrphDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?^TDATA ?^"DATA ?^SDATA ?^DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab.FVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE,LAST~~}JLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRDP1AMAiP1RLAdP1WDAmP2AMAvP2BAAA{ P2RLArPBASAATPBASAARwPCONAAb>PCONAA_PDMFTPDMFR[PLOCA[{PLOCAVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R<SCAN@SMED SMLtSMPL!!TSPACAfSPACRTSPACAfSVER4.0SVER ~SVER,,/Scal@Scan@TUBEC6Tmpr<UserphARphCHRrphDYbigphQLcphTRphTRV"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49WW!'YA EuzrH8s$bo%Rw\\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUhM'Sg+M2}AlVqCjrPCgBCQnl(}wp9Qlu19"c.VDTj.uFr~o[|!Y<,h`%mI{Mb=KubQ2a5qO Jm}Pz;+hj3ST?0sKG'BNp3<("Hf.Q F3eG"k@j/.3]J&sCe#&\7`[qDr_WG\|J_j.u%xfmFoM/V,<{1z^{5te.ER]QZEpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72Wei@8/(8\_pzN02%B4O7yr[q?Ib\fV9uDU03]/qf[ZL6`8dBw'JQEhXBN4UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4PCONAA_PDMFTPDMFR[PLOCA[{PLOCAVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%R<SCAN@SMED SMLtSMPL!!TSPACAfSPACRTSPACAfSVER4.0SVER ~SVER,,/Scal@Scan@TUBEC6Tmpr<UserphARphCHRrphDYbigphQLcphTRphTR