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  (((00477>>>EEELLLRRRYYX_^^eeejjjpopvvu{{z~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 60b7799b29e44b3fabee9fc3fe70a0d3Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-08_23-59_07593130Seq16818134909_134909mfagan96-Well#""####"#######"#""##"#"##"#####"######"##"##"############"##############"#####"##"######"#""#"#""######"######"############################"##"##""######"######"#####################################################################################"#############"#################################""####################################################"####"#######################################"###"#####"##########"################"##"#"########"#####"#######"########################"#############"#######"#############"##########"########"##########"#"##########"#####################################"########"""################"################################################"#"####################"####"#####"#########"####################################################################"####################""#####################################"#"##############################"#################"#"#"#################################################"#""#"#"#############"##################"#"####"#######"###############################"##########"###############"#############"######"####################"################"#####"###########################""#######"###########"######"#####################################################"########## 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Dz$5QF##('"!&&$   UE_&AR(F]8sR)#'+-//,$$iO5mk0S@!$&$ M  z^*             &h8*\'    T%GOVT4(&&$  [%%$=_A2+&" D*8!!LTO5-,*# F]>/z! S,!&'" Q#qq/k7 'Q :PI(X:)    M@M ObMM"    3pmf}F#  #(*(# &Om>qn6`Q/#$()'#!&&"!:rGB-@.?R.|!e"WY'kr0`^(`[5"      G2Ha4   KDka j q@#            :pm}))"vW9'  Ayj=a6     C%vhP-   +_WGj@&  L7~f"iD*      ={'~@f<"   0k&6Q>G_;(  $9Odt~xjYF5)! @KZV!        )>\DEUG8gXROKE;0%   +6>BA;0$)@>ACAA A KB.bcpKB_3730_POP7_BDTv3.mobtermAGAGGATGGCATCAAGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTTCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGCCGATGCTATGGAATTGAGCCTGTTCCTGGAGAAGACAATCAATATATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATCCCTACTGCGTATGTTAAAACTTTCGACGGTCCGCCTCACGGTATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGTGGTGGACTTGGAATTTTACTAAAGAGGATGGCATCAAGCTGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTTCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGCCGATGCTATGGAATTGAGCCTGTTCCTGGAGAAGACAATCAATATATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATCCCTACTGCGTATGTTAAAACTTTCGACGGTCCGCCTCACGGTATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGTGGTGGACTTGGAATTTTACTAA'=Pbnv*8DQ_o{!,8FP[gs*5?KYft} ,6BNYdq~$/;GT`n| "-9DO[ht+7DO[fs %3>IT`my  & 0 < I T _ k x  * 5 A N Z f r     & 3 ? 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UV1* 1L4:F:$BBGG?G_ ) 0:cBQ\ @O*'[^5:W9 B38XB]]dH_ DAS N(I. 4.*4'M/4@ =.-['[( MPAc=?X T: \F$E-5& B]bZBM#"]O*":-%JQ\%^/M E<Z .9'+W9WMR&L-]60& +L C0)&#:8A99<_$M^Z+Y[U VaL<SR2Q b(-4.:-S R,b,J>\Z!K55,CC.`&**^FF!$LH _Y1SF 25Q.3S YPc8XTI< ZbJI!?a8 ;J^7O@W'8KB"?2V*R258U/YR_SQcIOOH%VP)T@*cF)BAM8/!J_" TKBBDb:TD&L4" ,)[!Q3N].b#` =?N`` 9T=L 6]^426ORS>;>8('"@Uc"(J,JWG=S "'b TKFP2!; PR?:/!#By9-^ o-u@G;sP"$Y:$eh&4*TZ=}uYy`<l49xWu TX)}#W 7[ {y B[tawJm z@FC:8mVd_% FY ; E9 X*eOWdd{K`0B pdzv:#W0$4^r-\"yZ>V; T\L4AFTxvi\ ;^O]vh W  V)d)x1MdbB9'KC&o*l,AKf^+n8>Fdn .;,BsPE5^I 'V{0iHo S{ +: 6ex KCxy`YC ytmv-  | Hvv;@ -)(?q`SoVWQNHC93@=@.9/*:#6.2-#            "   &  *   %"  !",") ()!9$1&<). %5,%3$&3U35.#-$*)9/+1#>>02$#  'pp2b' G/"Ypa/pg<e,dtv,LI72Q;.2BOl>c/ttGIe1:+* DV":pe]}BDkfr> T\^5Hd# ,9dBA\)`?_$bHOI=v2a:G8<X64p},<j@ld;Mvu8f?~Map^'pCkZ H,$n,i7[pKN 8v c4Am$tl'pcWpmd,< n1r 9 HPAvP1bUj$J,4e+MRF2\5 0P=A3-0)Kd/=.N$(5%"%2.* !1&>!"!0##>9 I0-17G"0 P"A7%"1!(9A ;3#9;30  1'$'%#).<""&%!# .*'#*'"1(&'C6:#*$370#.+!+, )&#&*$$-%''#C;'()5H+5' /3$(' ))814&&*+ $># '#3-&%2>$$1+(#4" .1 "0!E)+3$7/'-($35I"83().91(.$0 *.&$<6gt3&,(%. (#7 ?$*9N?-4J<+"!"S,(!,$+ !# #C#,''(! 11*4=' 63#%384 -$"?&<'#83'+!+*3R3>@f4kb3"?GAGATTGTTATCTCGAGGTTGTGATGATAATATTTACCCACATAATACTCGGCAGTATAACCCAACGAATAGGATTATATGGTCGCATTCCAGAGAATTACTTCTCCGGGCTGTGCTGCGAAAGGAGGAGGAACCGGCTGAAGCGCCAACTTATCCTCGTGCAAGGCGAACATTTATGGGCCGAGCGAATTAACCTCCCTTCCTTGTTAACAGGGAAGGCTCATCGCCACGCCCGCGGCCGGTGGAAGAGGAGACCAATGAGGGGGTGGGGGAAGCGCCCTTTCCATTGTTCAAAAAGGAGGAAGGGCCAACTACGAGGCACCCTCCTTGGGAAGAAGGTATGGGGGTAAGCTCCGCGGGCCGTACTCTCCTAGGAAAGGAGAAACACCCATGTCCAGCTAAGAAAATCCCTGCATCGGCCGTTTTTCTTTCTGGATACGAAAAGCGAGATATGGAAACCAGGAGGCCCCTCCTTTTGCGCCCATAAACTACAATGGAGGGGGGGTTTTGATATTCTATCCCCAACGATAGAGAGCAAAATAAGGACGGCGGCCCTGAACTCGTAGTAAAACAATCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-09 01:46:35 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSgBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <yTDATA <yRDATA <yGDATA <ynDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~eLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS;P1AM@QP1RL@M7P1WD@V7P2AM@_7P2BA@@cP2RL@ZPBAS@@=wPBAS@@;7PCON@@JPCON@@HPDMFTPDMF;PLOC@D7PLOC@?PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%:SCAN@SMED SMLtSMPL!!TSPACAiSPAC;SPACAiSVER4.0SVER guSVER,,/Scal@Scan@TUBEB1Tmpr<UserphARphCH;2phDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSgBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <yTDATA <yRDATA <yGDATA <ynDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabuFVocuFWO_GATCFeat//uGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~eLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS;P1AM@QP1RL@M7P1WD@V7P2AM@_7P2BA@@cP2RL@ZPBAS@@=wPBAS@@;7PCON@@JPCON@@HPDMFTPDMF;PLOC@D7PLOC@?PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND  RUND  RUND  RUNT ;RUNT .RUNT &RUNT -:Rate 0GRunN""JbS/N%:SCAN@SMED SMLtSMPL!!TSPACAiSPAC;SPACAiSVER4.0SVER guSVER,,/Scal@Scan@TUBEB1Tmpr<UserphARphCH;2phDYbigphQLcphTRphTRr/)ecAA{!9Y2]C0nD2,3D?x%ZH|x+1[n\PF7^%4G}UlL`QF_4}V3Er]mz]~C*/meqy{g_hD6E{wsUfGX.!6^q;,`h?X??-|D>){6ft[XS<< x3]aC^D~i#aYP+p?H(+:MkO@JC,E`fW7.5k|_%Z]R'OEte1}1"/"g7bxd$XF${WhcS]U$3g;Z6ObePWz"$b[q5FaT%ycX}sP:gtqbg(*2K]?ZO~|Ue_v#a}_#S!KnMfgmwP99lvQWnT:e.*1@#K5>&jEp|9-$pVWlO@u+wGkSOj!Q4X+*flVinJils>:y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6 K*th ;L1^V'"FfzoW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'ngg61C`wWsV&1