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  $$$,,,444<<<DDCKKKRRQYYY___ffflllsrrxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 50fc10693b7b4137a941a142f6b2c8a7Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_23-50_07393130Seq16801134883_134883mfagan96-Well 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'4C=/>/>%%3G*3 '!A";%"$#*!!( 5&%*$!2%F3$   ()#  %   *!' +$&,(' !# ,'&*',%*%%&"+&%  '%%',$"' +&34/)/5063+<096*>=A>E;=0?9".>>6E8:87&-92@I17767>=A=97GBCC@97E)3D?FJ0DL8DQULTZgS<B\VTTJ?E>D:PH@TUQZSWLQNZTPKAHEBUTBTU]Igm_BQdYScjTPIQWSQ_XOZLXm{[YYWlxjhNWOnanXoz]YXw^}]a^`niyibi{mxpnrfTo}tW\\m\\uhxzsbsosyiYoffffhhhhfh\r\f\\\(>fjF FpWc:B; d -`pI^'5?,?m/^pddF,Ku&)|8DKG.SMad,dQ^|<4d|K&w@t%kXiW|pd$>cV,,4NI]]hGTApp0pWp Gfpmpp/puwxAppp$3pp2-qH` < V t:P~\&$ -Da]49D8) *(BX-.8ei(aEp!h43P4'#!?0k  # 'F dP"W#/Oh0s / #, !#%'%/8&#!  /(<D%)9-!&6+G7<0"' b[0*)<'(2&,&#& #+1$%189.2!A;:"!*0&*"%))?$ $ )-+'&)$1J17BE"/5)/!",6*:-'=F.!-#31., !8"92+5&0+7,4^0(41$)<M*AFD1V2:+N]'*)'3%T,7&'A*6*.:,5H%TB]:$28Kf,br7 ("#^15<G8K8,(:Oh:T"W/Bp"G#=M/B96X`WaH-?;*PdH("%@IWi/d=.r;&%>k9FzWq@bVKR'{J:`c]8?TFlBueCKuI,y8M@DAvveT/- QBIB <&p)8 iGAAAAAATAAATCTATAGCAGAGGGATCGCATATCATTACCACATTTGAATTATTACACCGTACCACCTAACACCAACGTTCCTAAACTCCATCTAGGAAACTGCGAATACGGAATGCCGCCCAGGGAAGGGTGGAATGATGTGCGGTAGAAATACTCTCCTGGCCTACAGACAGAGAACACGGACCTCGGACCAAACGGGCTTCCCTTCCACGGAAGGAATTTTCGTGAGAACCACCCCTTCCTCCACGGGGAAATGGGGAATACGCGCCTCAGGAGCTAGTTAGTCTCACTCCTCTGCAAGGAGCATCGTTACTTCACCGCCTCTTTCCAAAGTAAGGACGACACTTAGGACTTCAGCCCTTCACGGCCCAATTCAAACAACAAGAGGATAATCCGGATACAGCGCCGTTTCCTCCTCAACACTCTTTTCTTCTTGGCGAAGAGACAATAGTTACGCCGACCATATAACAATATTCGTTGCCTCAGAGTCAAGAATATTATGACACGAAATATTATCCATCACAACATCGGTGTATACCACTAGCTCAATAGTCTCTGGGGTTTTGACATAACCCACABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 01:37:57 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtmASPtmB1PtlB1PtlBCTS]bBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ;{vTDATA ;{v˰DATA ;{vBDATA ;{vDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~[LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS0P1AMDGuP1RLDBP1WDDKP2AMDU P2BADDYP2RLDPPBASDD3PBASDD0PCONDD@PCONDD>ePDMFTPDMF0PLOCD9PLOCD5UPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%0SCAN@SMED SMLtSMPL!!TySPACAj0SPAC0SPACAj0SVER4.0SVER ]WSVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCH0phDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtmASPtmB1PtlB1PtlBCTS]bBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ;{vTDATA ;{v˰DATA ;{vBDATA ;{vDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabkFVockFWO_GATCFeat//k}GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~[LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS0P1AMDGuP1RLDBP1WDDKP2AMDU P2BADDYP2RLDPPBASDD3PBASDD0PCONDD@PCONDD>ePDMFTPDMF0PLOCD9PLOCD5UPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%0SCAN@SMED SMLtSMPL!!TySPACAj0SPAC0SPACAj0SVER4.0SVER ]WSVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCH0phDYbigphQLcphTRphTR--)ob2=Y?Z8Z~]`m2W@V{%e_dQ<,N-AT$L8zX!3xn/+ O]rM701`jFB.VVQ8il6jJ}B~xGH$@iQ{nW)\lZhs| nG\|8 f&hPV)W)R6nL,rg`g6 ?63_`EzWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCO`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q I`X/13U`l${\Q`O96|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+F4$T9tR3==QC0^,!Sk2%vAfIq}ubsCnHmVNPn2uhv]qoU)m Pl: ,uk&q6X+9mMyTAFUTT3T&^%FyPC,_,qz7A*k|_LhvHZ&qIl4p&HS{8$w?D [;LY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d ClPzFtSEH$*YYo%@rs{6`Vs=y I/ax]]?EZp9}:>Ju6v;(vJnPkGr#d#"0Koiw87=6"4#O|!fEW<#G@njH[=K}2?Yk".Om,4oFo;X.pHwT_v^GgPmr87zKl@Bf-h{gvB#:=Jg$-KqN^Fu*te9r=u4TYe0+Vsp@O3sT6#f 6Y?03t0mP@*26G !9W&a:Ocdmo-JmE;9xxsWD@TZmU)P? sz{fzVSA]!lZbCd]'n,J6v`ZdD\4A,h{|dpwM5rm;p=_A26O1HnYDx8_b_l4P3LFRt( g>::#Qf60o'mQs&TY_sPW>nG!N`!$$RevlKor5#|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#ePDMFTPDMF0PLOCD9PLOCD5UPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%0SCAN@SMED SMLtSMPL!!TySPACAj0SPAC0SPACAj0SVER4.0SVER ]WSVER,,/Scal@Scan@TUBEG1Tmpr<UserphARphCH0phDYbigphQLcphTRphTR