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   5Ggl Vd,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8~}{ywuspomjhgec`_OOOGGHddd{{{   $$$,,,444<<<DDCKKKRRQYYY___ffflllsrrxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 559a66b041304a6ab90592e34192a5d0Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_23-50_07393130Seq16801134883_134883mfagan96-WellK1320_F5_Ficus_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z  zusrqomkihgfdb_\YXWXYZ[\]_beghfaYRLJLQW\__\XUSTW\`deffhkqx~}xvx~Cr*]Z_%j)c)}hTB2'!"""" !%)-00/,(&%&(*++(%"  !"#%'*-0369<>@@=:87:@IR\h{2{m Ru14>wbVPMLLLMPUY^abbbaba`\VNF?:76640*#  "&)*(# &-26640,(&&*5He7{Bu~IioL6.2C]4wd*)z&{*.6leH7.-07>DGHD?81*$  #(,/247;?CEC=5,$  !"        1Qv]Tu}+n *FK"          %MB"#1l1 ! 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[U<JSAX'Oc ?-5(,QTU 4\35B??/%a ;(\4!$R% OR+P) &CP>V;_O+G %Z692RcRc)RLT..&3;0CJ.+HK# Sb+DH;F# ?EI*9IWR R8C 4EV'3/3.T& ?'B%I 4(\#MR^<,IZ?QA_5F +:b:E>QO%XQJ2P0c224(?*DzZtX|xi z\I]\3}-ppYMSZ'ir/FZ;@(JEK@$dg )uf:2AQ3rsS`; c ,e_L,F(^NX0f1C6#aqN5LB<P={3Ty7p2}OZ_Q9'5 !]I <Bi9g2bUa#=)JBH7Gl O {$!-vV.P:iaIW\a} m_$7-s-x^{5z<:x)6Z:|scgcl0<Y-54`tL^#e g?m  ; 8Xs=;I4 G6?4p[ime)K|Fv{P[jL9FW|1o1FPiGmRw;&~JJr; FQ kXA0# .S* nQN/jYdkprlYl]}hPWQCZPAC>89QS3JC0)15D34SF912+/3(510$%%!&)!"*/3%"-!1 *#9" !%3 $+ !   $,*(#&- 7/)82#'   %  )  # !( !4)  +   &"!*  ! !!$"  $ "<*2'&"(8%'1@1 2(, 3*,37-.B18D6=3*/48>?7BI(-GAG=<P93+@?5%7 C;I:;9<Ea7*B<IB(J<GJ>4?C.O>GDZ9\AGLihdIN`Xh\TTPEJLE`QVfXhY`CNc[eRIIGJQ`gR[Z]CSWzQZ`d`p~wneWZ^gY^YYXhhhkdbP_y|NQdaqqWjd]_[fgjjDzuflxFffmrP2{|b `ao\{p}gSvvv\\\v\frfv'fff6"p]m-"v,&@qpic,p7X`p,Ivd,hZ.Ku,V\2s}|o8y8Sp"gk,l#%p>w pI,88#xpp ]b'YppWppppNdrCppPppDppppbd-j1]RXoHp&RX#5D8Bg]1?-V\-c(!P'X77a&$ <L%BZ%\*,&:"  $   "' %#!%" #!  2@&&&,%, [%%4*;#'!!2."( 6 #(7(C-!!.2!% =&  3<9',%96+9))#(/$<<-+'%)$,"1%M$$1\,6#*8,!#4?C!,:"!&:4 ".6VS8+>K}#/A))5+(>!..###),$:AG'/M/$`rH2d#-,CKLN:,_D5(/C^+D5AXuNVWaKZ@N4?RC8J);VL[9=\B))k'&Wj_MKEZS%(A7NB)m1SeAFT$%p~lrhgG/=YS'llxQisMS#:QA kj?n^P,>+'C+~$AAAAAATATAGTTCTATAGCAGACTCATAGCATATCATTATTAAATTTGGATTATTACACCGTACCATAAAACGCCGACCCTCCTGCACCAAATGTAGCACACCGCCAATAGAACGAGACGCCGGGGGAAGGGAGGCAGCGGAGGCGGCCGAAATACTTTCGGGTCCTACAGAAAGAACGGACAGATCGTGGACCAAACGGACTTAATTTTCACGGAAGGTGGGGGCTGGCGTCCCAGCCCATTCGCCACGGGGAAGTAGGGAAAAGGCGCCTCACTCGCTATCCCGTTACACTCCTCTTCAACGGCCCTCTCGACCAAACCGCATCCTTCCCCAGAGAGAACGAGAAAAAGGAGTTAGACACTACACACAGCAATATATACAACAATAGGATAAACAACATACATCTGTTTTTGCTCCTCCTGAAGATTTTCTTCTTGCTGAAGAGACACTCGGTACGCCGACGATATAACGATATCCGTCTCCTCATAGTGAAGAGTGTTATGAGTCTAGATATTATCCATCACAACATCGATGTATACCACACGCTCAATACTCTCTGGATTATGACATAACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 01:38:27 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt9ASPt9B1Pt8B1Pt8BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?VTDATA ?VDATA ?VS^DATA ?VҴDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE%LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMAhP1RLAdMP1WDAmQP2AMAvUP2BAAAzP2RLAqPBASAATPBASAAREPCONAAb PCONAA_PDMFTPDMFR)PLOCA[IPLOCAVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%R SCAN@SMED SMLtSMPLTySPACAedSPACR"SPACAedSVER4.0SVER ~SVER,,/Scal@Scan@TUBEF5Tmpr<UserphARphCHR@phDYbigphQLcphTRphTR7This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt9ASPt9B1Pt8B1Pt8BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?VTDATA ?VDATA ?VS^DATA ?VҴDCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE%LAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMAhP1RLAdMP1WDAmQP2AMAvUP2BAAAzP2RLAqPBASAATPBASAAREPCONAAb PCONAA_PDMFTPDMFR)PLOCA[IPLOCAVPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%R SCAN@SMED SMLtSMPLTySPACAedSPACR"SPACAedSVER4.0SVER ~SVER,,/Scal@Scan@TUBEF5Tmpr<UserphARphCHR@phDYbigphQLcphTRphTR[4\QWuMDVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95P3&5L(-3`]UTt53>94f%EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NHxr$Jg)=j-F!!PkBV$603:m_(=hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}OPDsvA\Fu*tnPl'Z9dgTiK]5bfUmb+9eDA0i}Ucyg\@>9K4ov.lN"@>oW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'84?73z<^*5 %6pebvM<$AJT[Mo"$+r:y`oW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#