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  $$$,,,444<<<DDCKKKRRQYYY___ffflllsrrxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 11fb693f0a8a4186bfd45667ed3f964eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_23-50_07393130Seq16801134883_134883mfagan96-Well'K1320_F2_Peperomia_consoquitlana_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z,48;>CHMSX_gpy{yyzzyvpjb\VQMJE?7,ypid`][YURNKIHHGGGHKR[fpx||yusuy~{wuvy| #Ab-7<8+eB r^M@5.*''())))*-28>DIMQU[bjpttqlgca`_^ZSKB92-*('''(*+-..-+*))*,/23458?Mc>~ Se*Zve[WZcq3X|b9~bK9+! 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'&2#. %+!'") !,A! +3 )(" (''<*+#=.;.? 7*.<  'UVQ7<)(E +(# )&+$;B+3H]< ,5) +  2"',#T/-3;) #7@ 7? 1c["=8@&))!0\m!,)%?&+2"<<%/<=K,%7,3J 1]WH%."P iQ('5U-&$>H'^8+@n!7G/E4A'O3I22([-n<9^2>~8uCx@;CF2!.F4Fjn!YBCSvfNR|KHtb*<UxQ_"~ttmG "~#R#n30/I&GATAAAAATACCTTATAGCAGACCTCAGAGAATATCAATACCTCCGTTGAATTATTACACCTTACCCCAGAACACCAACCTTCCTGCACCCAATATAGCAAACCTCCCATAATAGGCGCCCCCCGTGAAAGGGTGACCTACTACCCGCCCGAAGTAATTACAGAACCTTCAGACAGGGAGGTCACAAAGCGAATTAATCCCCCTGAACTTTCACGAAAGGCGGGGTTACCACTATGTCCCCTTCCTCCTCGGGGAAGTGGGGAATAGGATCCTCCCCGCCGAGCCAGTTAACCTCCTTTTCCACGGGAATGTGGACAAAAGAGTTTCTTTCCCCGTTTCGAACGAAACGTGGGAATTAATCCCTTCATTCTGTACTTCAAACTACTATAGAATAATATTCAGGTTACGCATTTTCTTCCTAATTACAATGCATTTGATGCCGATGAGATAAACAGTACGCCTTCGATATATGAATATCCGTTGCCTCATTGTCAAGAATATTATGTGATGACCTGTTATCAATCACATCATCAGTGTATTCCACATGATCAATTGTCTCTGAGTTTGTGTGCATAAGCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 01:38:00 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt_ASPt_B1Pt^B1Pt^BCTS{yBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?=~zTDATA ?=~z;DATA ?=~zQDATA ?=~z/DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~yLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNP1AMDeP1RLDaP1WDDjP2AMDs$P2BADDwP2RLDnPBASDDQ(PBASDDNPCONDD^PCONDD\|PDMFTPDMFNPLOCDWPLOCDSlPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%NSCAN@SMED SMLtSMPL((TySPACAhSPACNSPACAhSVER4.0SVER {nSVER,,/Scal@Scan@TUBEF2Tmpr<UserphARphCHNphDYbigphQLcphTRphTR!2This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt_ASPt_B1Pt^B1Pt^BCTS{yBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?=~zTDATA ?=~z;DATA ?=~zQDATA ?=~z/DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTabFVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~yLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNP1AMDeP1RLDaP1WDDjP2AMDs$P2BADDwP2RLDnPBASDDQ(PBASDDNPCONDD^PCONDD\|PDMFTPDMFNPLOCDWPLOCDSlPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%NSCAN@SMED SMLtSMPL((TySPACAhSPACNSPACAhSVER4.0SVER {nSVER,,/Scal@Scan@TUBEF2Tmpr<UserphARphCHNphDYbigphQLcphTRphTRk&zt Mirs{6`V5r@AKl&FaewMZ>fcn.=[|ebhktH4*&qve4q,8kf RCjxeCW?R5Ch2d'jw~|>J3zFReL#(78WFoe~8/zz-nX>z3KQv3.5s\yC!}WQ`Q5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ$zWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCo6T*-dotq$C9|f|Qc^F"P95Li2R~5OPh~jThh@<}9W#()P0&Mqzva].p>x'[vA-\K"L1{!Ul62dl=?o'%:`Kle8h/_Dea_';_/d@ikjFDfqcd o;X.>S[jWcgnW6Ob#7!VkWtt/9q$|jf+FJ9)P0j|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_1]?p,9CY6{A]i6OqH~|g|S['aum(]k@l'Z9dp"iK]jTj.uFgq%,NKo-jE(_y"%_oU@tC]2ZE<}|Uo%Z=r*r`sEY5_}4]'b5 ]{7HFwMzR93o^b,:V AmE$ww*{we*V&'Z9iS/oYFi$t.&"L4#,u6A|D2){|_nTJL:,aXj`Q Dx6m}>7:I1~8U'W33EZ'nO$`j|"/WQ`Q`zX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmpD0,_03gPV]O8oe])%|2CWllme[OCy KJFU'.QHT/}]66{6sA bBT9zH'zeE^O{U,\)lOY%}WeoVL6I-In(cy5+(,wd:!hBH(>CW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1nK-k]|sOJr4{6QuA!9@1%ilJG82G [7P^E$62:gt4zu99$(|fe0\2]S _T]A|'{`X/EM5Dj.6BC_RyQUb^ucF=W.X}=}F)h>S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0