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/"'(57*."$0  2!,)% $ .           d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8~}{ywuspomjhgec`_OOOGGHddd{{{   $$$,,,444<<<DDCKKKRRQYYY___ffflllsrrxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 28259c3316d8477c96499ea7ba8a3f83Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_23-50_07393130Seq16801134883_134883mfagan96-Well K1320_D5_Chaptalia_nutans_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z    zwvsnf]UOLJIFB><;=>??=;:9:;=?BEHJIGB=:9;AGLOOMJIJNU\cilosw*BXp"B\pzZ5 ]5s^MA96542.(" #%$" "$$$$#!"&+.122358>FP]n?)}SU_bnYKEDHPZgw@m  X+qYF7-%!  .KqL EqxJ p%{X>/+3ImI0"W ]MTm3y"tT>.$ $(+--,+)'$!!#$###$$%&&%%$$#"!   !!    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'*".'                 %"%#)"  !)# %%(+%.2'"#!)*'$261.0))..41+15,$,3)0"+*&=1568*23(-58-=A177&0.,@:'44/873<68;@@=>;76@*&:0:3?8<9:JHU8VND5@2UV-D?'A:;@EBS`7^7,BGHIPPPN>DIXYD6WCEaodRG]TQfi[kNQPPWVQTTUYbsZ^USxmfIQYYanUhh`s\`[apxadqrkotbowaajoqnpqy`wy `tzhvojVnffffhhhhfhazaf=aaa.pp 8 zXd/p.!/+!0I:B?sp7 t.ag U'h5?I`1#  >=LLl MAO%& #: "&D E&*!2,#36$8,/*($QO4/0(/>-'..3$,($6+ " %  * 5(J*-1%"3$!-*#  +?("# & 1&" 03>0*34<  A3&(.!!:*>I( .&26!!,.G= ()+*5+(I,#9$'#&9>+C@D,C+1-$W\-%-*!>,+6< +E$I 1 >X!2()R+p;!,\:FX8+@1=?!?T:3C*L?i{Ba8W`2$2/;Rf\U63'A /O/&!@?-Z\"Mv'D//8WT6>nUv>eV@_6WjMleRN8>J]e<dgV3MX_&];o?t}\BYaeh0. dP@\nA) F, JGAAAAATTAAAATCTATAGCAGAGGCAATCGAATATCAGGACCACCTTTGGATTATTACACAAAACCACAAAACACCAACCTACCTGCACCAAATAGAGGAAACCACCCATACGGCATGCCGCCCAACGAAGGGAGGCACGATGTACGCTAGCAATACTCATCTAACCTACAGGCAGAGATCACGGACCTCCGACCTTACCGCCTTACCTTCCACCGAAGGCCTCGGCGAGAGAACCACCCCATCCTCCACGGGGAAAAGTGGATTAGGCGCCTTAGTAGCTATTTAGTTTCACTCCTCTGCAAGCAGACGCTAGACTACAGAGCCGCGTTCCCTAGCAAGGACGAAAGCTGGGACTTCAGCCCTTCACAGCCCAATTCGTACAACAAGAGGATAATCAGGATACAGAGCCGTTTGCTGATCAGGATGTTTTTCTTGATGGCGAAGAGACTATAGTTAAGCCGACGATATAAGAATATTCCTTGCGTTAGTGTCCAGAGTATTAGGAGTCTAAATATTATCCATCACAACATCGGTGTATACCACAAGTGCCATACTCTCTGGGGTTTTGACATAAGCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 01:38:31 -04:00AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt4ASPt4B1Pt3B1Pt3BCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?TDATA ?DATA ?RDATA ?DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~|>LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMCgP1RLCc]P1WDCliP2AMCuuP2BACCyP2RLCpPBASCCSPBASCCQEPCONCCaPCONCC^PDMFTPDMFQ)PLOCCZQPLOCCUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%Q SCAN@SMED SMLtSMPL!!TySPACAdxSPACQ"SPACAdxSVER4.0SVER }SVER,,/Scal@Scan@TUBED5Tmpr<UserphARphCHQ@phDYbigphQLcphTRphTR4This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt4ASPt4B1Pt3B1Pt3BCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA ?TDATA ?DATA ?RDATA ?DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab"FVocFWO_GATCFeat//GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~|>LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMCgP1RLCc]P1WDCliP2AMCuuP2BACCyP2RLCpPBASCCSPBASCCQEPCONCCaPCONCC^PDMFTPDMFQ)PLOCCZQPLOCCUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%Q SCAN@SMED SMLtSMPL!!TySPACAdxSPACQ"SPACAdxSVER4.0SVER }SVER,,/Scal@Scan@TUBED5Tmpr<UserphARphCHQ@phDYbigphQLcphTRphTRRz0L/kbnE!0z^n3HVQQEO2astcZ~~VGC2lB)lW+Uf066}ZC__cIBM}.z zFef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`P|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTveurDmCq?`DQ`+}{Uplql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W@Vk1$#=4ZbZ4yM=VrTH&zqrua*b iq*#7rO/adu{>(Jt6qZ><}P?[NB@vulc*{w@p;#(":?S`,j.uFTcM#yKA#2rBA++m}Il4N&4|Mc$\*wQ[*gVHB (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flaO8JB6E$} {r*i`>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["#87zFl5xKbhh@l*#|h!9g-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW3hHmI(lV^+!:ZlB>1QW?+Z=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaxJK[Yl;j7V3]Hp~!U#LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMCgP1RLCc]P1WDCliP2AMCuuP2BACCyP2RLCpPBASCCSPBASCCQEPCONCCaPCONCC^PDMFTPDMFQ)PLOCCZQPLOCCUPSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%Q SCAN@SMED SMLtSMPL!!TySPACAdxSPACQ"SPACAdxSVER4.0SVER }SVER,,/Scal@Scan@TUBED5Tmpr<UserphARphCHQ@phDYbigphQLcphTRphTR