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  $$$,,,444<<<DDCKKKRRQYYY___ffflllsrrxxw}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 43735c83803f452086e3065ba45bdb46Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_23-50_07393130Seq16801134883_134883mfagan96-Well%K1320_A3_Peperomia_tetraphylla_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z3KZckx 4J]ksvy}|jYG6%pcXM@0 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" '  3$'5 0"4% &2 3) $(""&(52,*61#6+1"09# !-"1&4(".1,1"A#-+,2&*@%<$L!$,-%(6/,0=5.&/A"7' ,6&-7J2407+59/>0B)&455JP'?"*5><;BBO]3D)?99)5::_m::]::Z7:R?BV)))U)4PppppN{<MWdmY-[Xd^T;U2f+EJ|{Y_Zfq !9i !U 5d Y*.ph<e2ddd)sXO+l .~L8eKq_nY,(8[H14rp I1Yp 8@8w/O58 BUpAp> pgpf2 4&YpLE#p1zpY:gR{M;V $hru\h`5Z0-QL>{+QF-:B[ eu'N8 55ZEE{f#N8&B-"!a9")*i@&F5%F}C..6*)?0T,1"_.*+&6*] 5%0(o< ;03,.!$<'%f+@I*=<-?./P0-,63$23" (' !6$5.+>C1 031"2#6&(( ",J=-D1)1>*(!++1+..7)5W'8xr:1)#/6+Q:)%6#)CVaIA7+&!5^gF2/E(97y()c,GL>L~%2d.DdW((&L.!?0T$?6RFE*E]%9@K1<EL%L?"C,KE.4?%1>*':c;#9r4J52#^7,)A;G5<>+%"@C95MD;1V*?4C6Y3J0YOM16DwKaULL)8FN?1?F&4LC;M]T(lp>`ZEsD`Ui 2f5`TpG4C</Ik>o0(Z/)a[[6X88^A`SQCn-VqDJu?@[=6Ks1}&'}>1gsk=cK)}S--+AAAAAATTCACCAATAGCATACCTCATAGGTTATCAGAACGTCCGTTGAATGATCACACCTAACGGGTGCACACCAACACTCGCCAACCAACGGAGTGCAACTACAAATAAGAGGAACTATCATTGAATTGTTAAGAAACCGTTCTTCCGATGTAATCATTGGTACTTCATTCAGGTTGAGCGGACTTCCCCCCTAACTTATTGCCTTATCGGGGTCGGAAGTCTGGCGACCGTTTCCCTTTCCGCCACGGTTAAAGGGGGAAGGCGCGCCTCCCAGCCTATGGAGTGCCCCTTATGGACCGGGGCACGCTCGAAACCACAACTTCTTTCAATGTGACGAACTAGGCTTGTTAAATCATAAATTCATATTGTCGTTCAAATTTCAATAGCCTAATCTGCAGATACCGCTGTTTATTCCTTCTAAAGCTTTTTTTCTTGGCGAAGAGCCAAACCGTAAGAAGTTGATATATCTGTATCCCTTGCCTGATAATCTAGAATATTATCTGATTAACTGTTATCCATTACATCAGCCCAGGATACGACACCAGCAATAGTCTCTGAAGTAAGACATAATGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 01:38:22 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtRASPtRB1PtQB1PtQBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 3ufTDATA 3ufDATA 3uf"DATA 3uf}DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FWO_GATCGTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~OLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISoP1AM?~P1RL?~P1WD?~ P2AM?~P2BA??P2RL?~PBAS??PBAS??PCON??[PCON??PDMFTPDMFPLOC?~PLOC?~ PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%gSCAN@SMED SMLtSMPL&&TySPACAgGSPACSPACAgGSVER4.0SVER SVER,,/Scal@Scan@TUBEA3Tmpr<UserphARphCHphDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtRASPtRB1PtQB1PtQBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 1DATA &DATA 3ufTDATA 3ufDATA 3uf"DATA 3uf}DCHTDSamDySN 0lDye#DyeN0wDyeN0|DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0 EVNT0EVNT0,FTab+3FVoc+"FWO_GATCFeat//*GTyp!!0KHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~OLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISoP1AM?~P1RL?~P1WD?~ P2AM?~P2BA??P2RL?~PBAS??PBAS??PCON??[PCON??PDMFTPDMFPLOC?~PLOC?~ PSZE`PTYPJPXLBRGNmJ|RMXV1RMdNBXRMdV1RMdXBhRPrNJJRPrV1RUND RUND RUND RUND RUNT 25RUNT %/RUNT RUNT %)Rate 0?RunN""JZS/N%gSCAN@SMED SMLtSMPL&&TySPACAgGSPACSPACAgGSVER4.0SVER SVER,,/Scal@Scan@TUBEA3Tmpr<UserphARphCHphDYbigphQLcphTRphTROp]C$xXO+lkfc40-fKbVUg|=a1tirD:Z5A>9^kZdg~WI|Mb;eX}"if32#/P[~W!Fy^q3Th$Y i ^UO ]ddc}?8d)sGi=&MQJ1r]ZvZ~h6dYHa?yh;}S$PSD`^BnBCmJ1y;MmN2EmIz^{5te.ERbE2(5?fqcdsTF]lOTz9NKA\@:AV^V4?HV;mUCBM[?|$}d& 'iJ4~CubW_]?ZC'T3T&#ONx8LkV}d7?#)io5`N0W`,#Xr_<.Fi1A+7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\W\lWQ7M3gptQ>?";!8L[2Rr`qHT^1R=goQS-CDiyX^UM]N=?W.ss$T!D@4ir$Gj~`? MlYXjlnK[Yl`A #pJE,#VuU4zrfv4AAn_/z9{=LnHIWysh>0N}5!){mnjH[v]Eb,8HW>ycPJ~FT,{1:Kn6?_s2n`457^^sIdwz@{&]t[X'(A(f'PJA}*4d#kE_GY@ZWeoPTF}cyR6A;{IF}o-jAS_7ea_';_/Ft(#j1DjU5]DtmXY#agsC!.:QI5&.XRz+*}Lq&Wi1Wc]zI**pENuiV(#:s:A_hEKwQQ;~O2[4mg$seZD5u/03BSmv^LoT.iP""#]eYWjoW`$~~hY8|kN_uw >xP!t p7qf j O5TwARM[OCy KJFU'.QHT]R06`NV%]?EZX/-k'iJ4~CubWk@X=<-8 )T;Fi$t_SF`|zo;LY&PE!am&,*oe?F[Z*`E }Z&[g;z%!SNmW.$7KO],n`!3o %>