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L,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|zxwurqnljhfec`_OOOGGGddd{{{   $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 67ba8a701e224f42921f9f7b985608e4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-WellK1320_G8_Lepidium_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z&/;Mg-E\q"(,-(oQ/ dJ4! 4Mj5];]y~kS7tfYK=.mO2{qljigc]UNIGHKORSROKHFDCCDDCCCCDFIKKJGEDFKS\dhkmr|K;}*1*V],zkc``abcfo2x|; [0 FsX\ZN]E Mmh@(<mhH3'" !""$&'(('&%$$%(,159<?AEJOSVUQLE@<977:BRn WE9>cKBH_] ~""C0dC2,,--*%"$$"! !"####$'*.//+&!$3QFl BJG#tD'$'())((%!      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YROR,BJU+@*7D@ * N/W9T2#@SG3?H$-5;0,*`Q\/.=*J=aIA/84\'NTO^ :"T(-E< ` FJVDI/Q'X)UL/][/>`EPS]6W]G13]a@]B1@QWccTSB' c!$FEa`]-0" $;2DjiWd<|wN_UnbZ{cm^@*hLSA5g)eZa=gCGiVvs~7vH!.j   ~BpEFaO_RldK trr.r%d6^" `~}VX!rx3U$-iB O`=Bj.a~0{3CaZbp$NX+2 ;;iaL"!K3Vkaid'G@@y7GF|/_+B,!A>03/G$D' As A}Ys$oKJ"wvymds*XQ\J"u9O[4vN-Wp g-=ngb1&u^ xr*cl)cR lB=PpNp 7aMP7/5x<}spuyoc~ept]lpQI)R`K.{qMIJG/0EEAA?;5:4@5('.(3522&!""+-73'/34##(-#/ (B&0!#()&,=!%   (  % %  "   %!!$("% $5"+  &*+*!!/ ',*%#($/)0%' @9E@24$5!%3')#=3*4/9G71%,FC=;G-87'F-:177-DC2G=7.?/.87?PI[XLcccMWWcHWTLZWI[YOYY[NTbJYPZURR@]pVvbhudignn]P_X~etvvdyl}nkSn}gXmsrqmswqhovtp}ttiiix&~u|yiir<iii^Iai1z]ppq6t6 5f4ZVp|(m)NpE+Y3~WK=,8L)p+3,w\PF!3r4mC5Y*#%ll?p\P,pBY1p;p6 3dXpvU+4~pp!pp)pKF !pppL p k9pH y<$Shpg?AOJn2O7/IQsOli4E47$Gv%#M8k1j{#+O>M %!*:-(4!& 2%)CL  SB$""39 4Q1*#0)6#'& :,9)A+C9$6"'F2.5$2'6$":6( #05C&=1$:8_WO/'78.-6(*//&"#'$@b & %+$((*-#*3:&-*7'#<8;&ZF 0-"C@(%   %,03D  "/Fa6 %+(;7/fJ--X9:$3#16&1 .#-;9'(1*$B)8+5>A>+9:08.+6:++2&=4F#6Y:D5+[828">69R0"/(F=F|-L2X74IE;&`HkM[L#VD$,FW6;," _[I3t\<NO@ %B%(#MYDL]DV9H%ynPU&=o[%TkaeTGM@TL+_H@qdf"]u&8-zVgGS~>wK}aanzG"CLc]c<8kz%S}  GAAATGGTATACTCCAGGTTGTGATGAGAGTATTTACCCACATAATATACTGAAGTATAAGCCAACGAATACACTTATATGGCCGCCGTCCGACGAATCACTTAACCGGGCTGACCTACTTAAGGAGGCGGCCCCGCCGTACCCGCCTAATTATCCTCGGGATCTAGGAAGATTGGTGTGCCGAGTGGATTTCCCAACCTTCATGGTCCAGAGGGAAGAAGGATCCTTTTGGTACCGTTCCCTAGAAGAGGAGGCAAACTGAGGGGCGAAGGAACCGCCCAAACCAGCTTTCCAAAAGGAGGATGGGCTCACAACGAGATCCACATCTTTAGTGACTGTTATAGGGGTTTAATCCCCTTTGCCGCCTCTCCTTCTAGAGACTATCCGACTATTTCTTGCTAATACAATCTCACCCTACGACCAATTTCTTATTGTATTCGTGGTGGGAGATATTTACAGCACGTATACACACCTTTTATAAGAATATGCAATAATCCAACGGTAGTAATAATATTCGAGGAGCAATATTGGAGAGGTGAATAAGTATGTACACCCTGTACCTTATTATATTCAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:51:00 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtGASPtGB1PtFB1PtFBCTS{BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?^~TDATA ?^~IDATA ?^~RDATA ?^~DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE4LAST~~zRLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISOP1AM>|f$P1RL>|aP1WD>|jP2AM>|sP2BA>>xP2RL>|oPBAS>>QPBAS>>OPCON>>_jPCON>>],PDMFTmPDMFOPLOC>|XPLOC>|T4PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%O}SCAN@SMED SMLtSMPLTQSPACAh5SPACOSPACAh5SVER4.0SVER {SVER,,/Scal@Scan@TUBEG8Tmpr<UserphARphCHOphDYbigphQLcphTRphTR+This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtGASPtGB1PtFB1PtFBCTS{BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?^~TDATA ?^~IDATA ?^~RDATA ?^~DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab6FVoc%FWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE4LAST~~zRLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISOP1AM>|f$P1RL>|aP1WD>|jP2AM>|sP2BA>>xP2RL>|oPBAS>>QPBAS>>OPCON>>_jPCON>>],PDMFTmPDMFOPLOC>|XPLOC>|T4PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%O}SCAN@SMED SMLtSMPLTQSPACAh5SPACOSPACAh5SVER4.0SVER {SVER,,/Scal@Scan@TUBEG8Tmpr<UserphARphCHOphDYbigphQLcphTRphTR(w}Uzrla@O(LxC[>({]>REd7gbOHG'~[ABMNT@bxj[Dbt6XhVx9zG@[76gg`qC+lkfc40-fKbVU}MS{p[v[!s.#g;X6YM'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ-,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM1voDGG5)ljD_n?Kf]4'Uw\Pcc{\A_}$A>AR5Z(>B|::zWTR0Xwt2)lOY^k42g|5 x*.JIc\<:!:B_EeLHo5m"WlQ8UWMM`$M(P@>/D?*Uq$[-o9D4xH/w3KF;LsqBlC4hETI#daC.?bFW&a:OcPX*F8BjhsDgn_RJ4ir$GE1Xz.?e Ml1}ssb[l"G>=?]p&k6v)ukO"yA&d}8qQgE5]U'j*-[~!Tq*nEg8Z~KI'_26?^V$Yeaejp~{>ZHm? >#veu]@|Z5hf6]X?K90#qN8>o XS_xP=O_l;mq;;9VgO(_y"`k:!.tkSY4E2 (# +6hjHepVIy{KGk;_!.V&S$~=}F)h>;-T)RJTZmU)P? sIe]bZ]"Po1Cd}F<_`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQLAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtGASPtGAUDTIB1PtFB1PtFBCTS{BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?^~TDATA ?^~IDATA ?^~RDATA ?^~DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab6FVoc%FWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE4LAST~~zRLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISOP1AM>|f$P1RL>|aP1WD>|jP2AM>|sP2BA>>xP2RL>|oPBAS>>QPBAS>>OPCON>>_jPCON>>],PDMFTmPDMFOPLOC>|XPLOC>|T4PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%O}SCAN@SMED SMLtSMPLTQSPACAh5SPACOSPACAh5SVER4.0SVER {SVER,,/Scal@Scan@TUBEG8Tmpr<UserphARphCHOphDYbigphQLcphTRphTR