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%         L,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|zxwurqnljhfec`_OOOGGGddd{{{   $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2faef87e00c048fd80a107bb6c9732e9Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-Well"K1320_G2_Peperomia_botterii_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zOUau2Nfx~iXI8% !<\/PjhR<&zgQ6oL* uh^ZY[_cgjkllljhgeb_ZUPNNQTWWUQNMMNOPPRU[blvH|0nY_4~zz~H%z&t j@  DuCSh29su2 1;(U"q)vbULGB<6/(" "%(*-.//.-----,+++,-.//29HbB)q;K hQ@3+%!!$(+.//-*&$!  !!  #')*)&" "$',5ARf|r`SIA:3-'"  "&+/48<@AA@=94/)""$$"     #$$"    !    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GX*/!w/&j"#Lv(14t+&"j766a)xG&46EEb386+knN!$J%.6S\<9#9,$K=9L&U5o ?)mxON,PXR3eIA:[>]7BRU{hH9=p{SSzR=mZ7>{_nq"wuwa{y *z'wa {@zu?D!7*zKQ)Z{FI&?Yi7e!6HZk6W0)-AAATTGTTGTATCTTTAGCTTGTGATGATAACACCGACTCACATAATATACTAACTTATACCCGTTCGGATACAAATATAACAACGTCGTCCTTCTAGGCAATTCTCGAACCCTTGAATTTTTTGAAGGAGTAATCGACGGAGTTGCCGTAGGATTATATTAATACATAAACATCAAAATACCCAGAGTTTTTAAGAAGTTTAAACCACAATACGGCAAGTTGTTCTTGTTTACCCTTTAAGGCTATAAAGGAGTGGAACGAGCGAGGCTGGCTATCGGATCTTCCAGATTTAAACCAGGAGGATGCGTTAACAGTCATAAGAACTACTACAGTGAATGTCCTGTACGTAACCTCGGCTTTCCATTATCCACAAATAAAGGATATCCATTTAATTCTGCCGAATAGTACGGCAAACTACGATTGTTATCTTCTTTTATACGTGAAGCCTTATATTTAAAGCACGTATATTGTTGTACTATAAACTTCTGCAATAATGCAACGGTGAATTTGATATTCTATGAGCTATGCTCGTGAGCTGAACAAGTGTGTATGCGCTGTACCGTGTAAAAACACGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:50:19 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtRASPtRB1PtQB1PtQBCTSnBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ={vTDATA ={v DATA ={vKDATA ={vDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~mgLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISBtP1AM@Y'P1RL@TP1WD@]P2AM@fP2BA@@k'P2RL@b'PBAS@@DPBAS@@BPCON@@RgPCON@@P'PDMFTtPDMFBPLOC@KPLOC@G'PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%BlSCAN@SMED SMLtSMPL##TQSPACAjSPACBSPACAjSVER4.0SVER nSVER,,/Scal@Scan@TUBEG2Tmpr<UserphARphCHBphDYbigphQLcphTRphTR/This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtRASPtRB1PtQB1PtQBCTSnBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ={vTDATA ={v DATA ={vKDATA ={vDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab}KFVoc}:FWO_GATCFeat//} GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~mgLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISBtP1AM@Y'P1RL@TP1WD@]P2AM@fP2BA@@k'P2RL@b'PBAS@@DPBAS@@BPCON@@RgPCON@@P'PDMFTtPDMFBPLOC@KPLOC@G'PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%BlSCAN@SMED SMLtSMPL##TQSPACAjSPACBSPACAjSVER4.0SVER nSVER,,/Scal@Scan@TUBEG2Tmpr<UserphARphCHBphDYbigphQLcphTRphTRc:]FN}T3pb}045@u:r*nOroz*N^YEu'+=3yHSBrRLohL)N6PagEU0T6;e=Y"sWi|Cal(i#5h1g9%TFIO?z3e]/\(fp%Jp,@jJo{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^HApEnFiWb)jN-:d=iU~Hfzq?;o4OQMSP{4RMJ#1QW3Yp[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRb 2)FB[yEZ>f-6>C#!^Cu_OlYc?pTtfQgvv6=#Iw}~Fc[o}7YD8gw.^^?:L] IT$ei^gsm2f*7k'}&8)TK7kx`rxP!t p7Zf_@72tX>VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8K%}jxz)>`VWYhY8|kRYN4+HmLP}6YH4HUXYnuPQh^fP%4X+*flT)FTX>EbrB%P^gWT|0\d&nPl'Z9dp"iK]|UKo9UC|T[[66`aD/HO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX>V