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  $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 47dc76f329ec48f3bc5ada284c3407a3Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-Well!K1320_F11_Indeterminate_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z#BVblz'9K]q      qZ@$gK2  %DeF|'V~ydP=)vcRA0 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((-") $#!+($&,02")* #4-40634.,%+) ')1*23&(7-50'4;45:.*;;35;mD:F4.>>>@NHRZROP8<@BC95?I:KBLRCTVVVAY>MT]OLWQJU\_K]rZdJMM`WKX_osoguuZdnlmXky[oRme\jhdvylzeieccwnnqzm[ooar~!rudyt~kxqyyp&Hx K1jtTpp;UW(pE,]QlG8SsQBop?58LG1T};\EvBi\:OB&wp8c&o|@Gn8'qt/ZV/q!wP;8Hp c#ipXp$pv ')pypxS/}ZUh&%kv=5e%>f*1JAKInu7)?))Lc0&`Z1 *DA?"&+&"(8%%#SI*)=,++3;M"A)=rgV4""-971b/7%%B5$*,& %0'3 %)I*|6-)4 $#b[># +Kdj['#!%:0 0*C+"1-"$;&74)E4*&/,)="-&8LK"(1Q"(+.)B7)$(M>2%(A6504.:(,@/&4Te#.=$D7$G1@V^5K9,72"F1>7<7IZF&M%,)^LxBN$:xq.4*Aa?]L]I?MkKFQ#MjL3(3O0t($bI@!tKFUO^X>YPQ6:E3 Th{VA?T>g3u!jQo%NRL{E[ ;kR|wu.i!M(`gDfft,{lVx[h_CO1cX'.Be}Kwkc%2c:B &(:A0KT;i;p ,T$8*g"GGAAGGGGTTCTCGGCGGTTGTGATGATAATATTTACCCACATAATATTCATCAGTATACCGCAACGCTTACAACTATATGGCTACAATAAATTTCATCAAATAACGATCCAGACCTTCGGAAGGAGGCGCCTTCGAAGGTACCGCCTTAGTAACCGCCGATAACATGCACATGGACAGGCCCAGAGATTTACCCAAGTTTAGTGCTTACCACGGAAGTAGACTCGAAATTAACGTATCCACTTGCAGAGGACACTAACGAGAGAGCGACTATCGCGAATTTATGATTCATAACCCCGGAGGCTGGTCCGACACCCAGGCACCCACCTTCGAAATATGTTATGTACGTATGCACGTCGTTCCGAACGCTCCTCCTGTAGGATAAGCGAGTATGGCCACCGCATATGATCCCACCCTAGACTGTTCTTCTTTCTGTATACGAAGTGAGAGATATGTAGACCACGAATATTGTTCTTTTGTGGTAATATGCAATAATGCATATGTAGATTTGATATTCGATCCCCAACACTGGAGATCGTGATAAGTATGTGCTCTTTGTACACTGATAAAATCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:51:29 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt}ASPt}B1Pt|B1Pt|BCTSNBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9slTDATA 9slDATA 9sl;kDATA 9slDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~MLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS"KP1AM=z8P1RL=z4fP1WD=z=ZP2AM=zFNP2BA==JP2RL=zAPBAS==$PBAS=="~PCON==2)PCON==/PDMFTsPDMF"bPLOC=z+rPLOC=z&PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%"CSCAN@SMED SMLtSMPL""TQSPACAo/SPAC"[SPACAo/SVER4.0SVER NSVER,,/Scal@Scan@TUBEF11Tmpr<UserphARphCH"yphDYbigphQLcphTRphTR .This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt}ASPt}B1Pt|B1Pt|BCTSNBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9slTDATA 9slDATA 9sl;kDATA 9slDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab\FVoc\FWO_GATCFeat//\GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEULAST~~MLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS"KP1AM=z8P1RL=z4fP1WD=z=ZP2AM=zFNP2BA==JP2RL=zAPBAS==$PBAS=="~PCON==2)PCON==/PDMFTsPDMF"bPLOC=z+rPLOC=z&PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%"CSCAN@SMED SMLtSMPL""TQSPACAo/SPAC"[SPACAo/SVER4.0SVER NSVER,,/Scal@Scan@TUBEF11Tmpr<UserphARphCH"yphDYbigphQLcphTRphTReih4qH4l|t2#OoV$ zrTbZ?a23|jyHAj}W2yv!)QBmO7]N=]TG4/CeG1{?{sb: h,`,l6IEUY[^wA[h@t#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`K\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nvP?QYMA67E |VzM:*]Q{Y^@>=,8,?qm#v6F[(vW3TA=]I:<{i(os1tog,s48h(PW[9?Db2@&'n[Nn%9SXWl573!dX!%~AfzvrfzoB=Si\u 8MjHbinOY5_}4]'>'s1HTf{fzja:3AG}X`=/r.ZNum#0kc "7:I12gbXW,62Q$OEHbX\"BV_@iD]xy"^O[,z^E$6d+XnhcjPGh{;qeXd9UZm0UlBF)OM}A|tr#~w"jxtG=*QepR}Wgn#u]9g~~hY8|kb+N4+0Oj!Q4M"}JMzR9vAMV^@E P:i~8m+adJsOlG6]}fWG\|J_b.O_l;,[[`7VAd#RBK9JX@{F`!~6I57%6C}B\I/v]-@6|k.j,PCH-pC+lkfc4hACI8-W=;OF>BrRL8~f)NKo-j5t9z"H~:HziPtJdQex-U[dPz;+hj3T'7_6t;:!8/.TrTs1fi;+^p0Fp|J"9,)&'m#ulc*{g@]?#(lCKOJ_H#[,bTmoNH'a,k){_&jEfF:,\@\=5NDzU4E~z%QrcDpN$t.Dkln{HbX\"BmZ=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaxJK[Yl;j7V3]Hp~!U#