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"        1(26/>,>3                     L,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|zxwurqnljhfec`_OOOGGGddd{{{   $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 54c7a1e12e3d410790d59cafb60c20c7Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-Well(K1320_E5_Mildella_intramarginalis_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z !:`(U3Nl!?Xn~jXI;,rX;{Q'~eO>1)%&,7Ia~ 6iKuwY7fJ-wW6wlc]YWWWVSOKFCA?=;96445689999::<=?@BCEHKOSWZ^cm|3f\4KTO>!}4e/xrnmou2uv,Ilvg?J#  9Wxk#pn.[ {cVnc9&7b rWD92126;>@?;60*&$##"! 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(H]O%B&XUJD:=W7 ; T+-31b*$[1\W #<FMB$-]"A;*bA.5S0V2.# >'H/4F77Oa 4>O.Y3' 96R+%:Y9K.8&QV_Y1A9.GK"W52$9^)C8 BNaY:#L;IQ0M?=`(2T"H N>.Pb/ %;S8QR]SM3<K!!P];cH0(&S ="[P+[5*<J!I"cR0IFJ92C $  80#T"@.K'I@ 5R,[^)-FC.V"4RM".=$22<X>=U+ GJP9+'AUOE164@7C;?"5CSbW2Q 6`^2W&$+b`PLS":`'!2M #'.W 5FW F/E:2)(b^!go_$_ [297N1 ak&?S{[ XMj= ^psVVk@};T.*(< p~9Dgp E6I_ cdKQ'PDZJ;(Q ,*t{(lG/@f8V8& h0{i?; Dt&OcjYVW)Wj E8)ef24Qc6;=>)3[~D,a U FGG.+)WihY]uc.q[\eMu5,C4X_:<4^[?} hORGWa"DT #76St.tgT8?F} V[8d'pMKV{)(^K*Fyh?dke+5<- Lx-*#/*P|F[5EG SxL m)M~!IcoJ_?; 4Y'T}b.3 #mv(|skbcq\crY\Oi@ZAAQHFC4;;4@E527,)$/D&.+.*%11!0+$"  %$(&- (,"$ #!% #+!*%    " $! "  +!"!%' &!!$&,!('+$,.-0$+(3% "3#*%*,)42&9+6 '3550.H:4 )0=:.;6:<C.4S3><92<G':W9731C>7CHF(DES4????I@P:GLRYML$RRSIGK@PGX;WOJxV`Wea_bZ]SDQF_UN]rYd}PXbgb\Ukj|rHx{ran}qmYazuv~ko]ijwxQitnw|p|l~pz m5^zUsglerrrrrrr `yrrrrrr5rRRdByppp>8p/8oN}pldpvd,oGCMpPRXp@dp>Lp8pQi.9p&8HqXj 5,pb],uN,YMpS ;.2}Tpppmpp{d;w T[wdppTjp,pp[s6 v\Z+6CrUW3MH,McDt7wU .33,pz$ CO\D$ Ka9/BHD0 !-<.$\h#\8L!a*) %(, *(0--!$$! !C)& 6* 5-$1% N 4(+   '4' +!  "(/'5-I  "-"7*%+-E$8+'!5+.4@-{'5%)/4(9",6-5-+8!,:/ /*W,8]G[2,%0FM&&(1588KZA*F&3`hm/*"<AV`8*X3%=d3-nQfFG9a7aBE+xtCjq,W2@bT)SQQA =9h3(?,kFM$A[qUhOq+.pqZT^OKQg[>7M#sW}+LEt y@vJn9OM)]X(("z{~msiY.[07@M; 3 f+ l\GAAATGGTTTACTCGCGGTTGTCTCGATAACAAATAGACATATAATACACGTTCGTACACGACAACGGACACACTTATATCGGTAACGGGCTTTGGGACAAATTACCAACAAGAACAACCTCCCGAGGCGAAAGATCCGTAATATCCGCATTAACCTCTTTTTACACCCACAACATAGTGCTCAGAGACATAACTTCCAGTCCTTATCTCCACGAAAGGCAACGATAATGTACAGCGGGCCGTTGTAAAGGATATTAAGGATATATAGTACAAATAGAATATTCTGATTACATACCCGAAGGATGGTGCCACAACCATAAGCCCACCTCTGGTGGCTGTTATGACAGTATAAACGACGTTCCACGCGCTCCGCGTCTAGGACATATAAGTACTTCTGCCGCATACTACCCCAGAAATAGCCGTAATTTCTTTTATACGGATGTAGGGACATATGCACTCCACCTACTCACATGTTTTCCACTTATATGCACGAACGCGACGGTAGAACGCATATCCGACGGGTAACACTGTAGAGGTGAATAAGTGCGTCCTCCCTGTACCGTGTAATATACCAAAAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:50:48 -04:00AEPt@]AEPt@]APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt3ASPt3B1Pt2B1Pt2BCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?\TDATA ?\DATA ?\SRDATA ?\ҮDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~}>LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMChP1RLCd]P1WDCmiP2AMCvuP2BACCzP2RLCqPBASCCTPBASCCREPCONCCbPCONCC_PDMFTzPDMFR)PLOCC[QPLOCCVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%R SCAN@SMED SMLtSMPL))TQSPACAe*[SPACR"SPACAe*[SVER4.0SVER ~SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHR@phDYbigphQLcphTRphTR-This is the confidence range *ABI_Limits..FVocAEPt@]AEPt@]APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt3ASPt3B1Pt2B1Pt2BCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?\TDATA ?\DATA ?\SRDATA ?\ҮDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab"FVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~}>LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMChP1RLCd]P1WDCmiP2AMCvuP2BACCzP2RLCqPBASCCTPBASCCREPCONCCbPCONCC_PDMFTzPDMFR)PLOCC[QPLOCCVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%R SCAN@SMED SMLtSMPL))TQSPACAe*[SPACR"SPACAe*[SVER4.0SVER ~SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHR@phDYbigphQLcphTRphTRR)U`l5j!X`7.qT*uq&6i}8Amabj1A6rd)ziA=sUOzj{>ZHm? V<(?:13Uv|Q{9V \+Yoe2{sGi=&u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbN-&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`G]p-@Qhiq|fq'#kD:_f7o,f9r;qBwJUo6;^UWZi7WeOzJu%~4%rIZHp#)+KT>:L-Ev!$V@AKl&O`x Pz5's1Hhl@4t/9p$=3IpFs9$;+V5[~7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MKl>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdO#rT94Bvo?dez>T8xS$`Cfm;Ly?a8dYh~{x@~[4mB,F7RP 5Go-D7I 9xxsWD@4ir$GE1Xz.:LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMChP1RLCd]P1WDCmiP2AMCvuP2BACCzP2RLCqPBASCCTPBASCCREPCONCCbPCONCC_PDMFTzPDMFR)PLOCC[QPLOCCVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%R SCAN@SMED SMLtSMPL))TQSPACAe*[SPACR"SPACAe*[SVER4.0SVER ~SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHR@phDYbigphQLcphTRphTR