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L,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|zxwurqnljhfec`_OOOGGGddd{{{   $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4a9c4b84675b4770b522fb23db563013Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-Well%K1320_D4_Asclepias_curassavica_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zCHSf -;IVbmv|}zxxy||Z5wY80U <qFr gK/vaI/vQ- yne^YWWXYYXVTQOOOPPONKIGFFFGHGFB=830/024689;>BGMU_m,[Ec1{>|iZMC<756<ETg9n/YunS6 Cs6vE:B .f]u 076kSvc[WUQKD>:777876420.-./258;?BFIKLKGC?<;;<=<<?H[z#n Rz?ciJ;;Jeah]PfPA7/)&$#"      *MKsXiEwG'g1        0rjwN],6Vv6 !'++(!   6hHnF         *^ ~Qn:z; "#" "$$#!  \~O-Q NR  "&())'%#  ^}aR3M cT{T#  !%% %Bh RX#C   BvHzyGZ% 0n,0q2   !$')*)%     ba$aSn0 "O7r5"~&5p5 #'&!    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'"%"*#&& ) &&'&&'$. $#!,.#"#*""'( 0,,#%)$-/2-(.)&0! $1246>&  /@<FF@?.Q0-<;?-.6FE5<KB?BE<?;M2>Nf5?J@DOLZ@NN:X;J77E6AGRV2k}XM@OLbS?[NaFVDfh?WTv`^r`k[Xf{tbeg|fPPPmuwoip]j{iyvuv|b~g^joqj~wowmwz^|nz~xz ( `{qmp#pppppH"888Q#pp,$pI 1X8_|,d:LrTgI]umvzp|8E8 P*sp~pp`TCMN pv)Lp pOp"p]1;p9pp1ppQ p_ pN>&ZF`=uFVW&o=.[D>bj01;9ij#4| rj(")4)M6*+!;*,O&J& C'(&e#t#!# (!$% ,"!1!/  %G  'H!#)6'.,%* &'0#!"3-+.$+8-,4044;1 -& +*&!'$&!6%:7$+!"#)-4DK+!'!WH$<8/:!@U@6(4$-($u&0X,/,98 P[C""-+(5QM2;*$=2J+1+A"5,8v!*)te- !OMAL5IpMedF&l|AD4Q15Vn$$n>9fFLO&=N8Np% ?q2>Wf>A]e~wqs:XuEfoMdY3O?4Z]XGu?7(!G Gin(-"&Clu?36q+y`O_K}q<5DM X!^EGGATGTGTGTCTCGAGGCTGTGATTAGTGCACTTAAACACATAATATACATCCCTACAAGGCACTTCATACAAATATATAGCTTGCGTACTTTGCATCAAATCACCAAACCGAGCGTCATCCCGAGGAATACTCGGTGCTCGCTCCGTACTCAAATCTTCACACGGAAACACGACACCGTACAGAGGAACAACCTTTCTTCGCTATCAACACTGAACGAGGCGACGTTACCTCGTTGACCCTGGTCGAGGAGGATAACGATATAATGAATGAATAGAAGATTAACGACATCAACCAGGAGGATGGTGTGCCAGTCACAAGCGCACCTACACCAGATGTGATGTAGGTATGCAGGTCGTTCCACGCGCTACACCTCCAGGATAAACGAGTATTACTTCCTCATATAATGTCATCATACGACGTTCGTTTTATTTTATGCATGCTGAGAGATATGTAAAGCACCTATACTGTTCTTCTGTGGCCATATGCAATAATCCAACTGTACCAATGATATGCGATTCGCACTATTGTAACGATGAATAAGTATGTCCTCTCTGTACCGTATAAAAACAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:50:37 -04:00AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~:BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?VTDATA ?VDATA ?VSCDATA ?VҙDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM=zhP1RL=zdP1WD=zmP2AM=zuP2BA==ztP2RL=zqPBAS==TgPBAS==R*PCON==aPCON==_PDMFTwPDMFRPLOC=z[PLOC=zVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL&&TQSPACAdSPACRSPACAdSVER4.0SVER ~/SVER,,/Scal@Scan@TUBED4Tmpr<UserphARphCHR%phDYbigphQLcphTRphTR)This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~:BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?VTDATA ?VDATA ?VSCDATA ?VҙDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//UGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM=zhP1RL=zdP1WD=zmP2AM=zuP2BA==ztP2RL=zqPBAS==TgPBAS==R*PCON==aPCON==_PDMFTwPDMFRPLOC=z[PLOC=zVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL&&TQSPACAdSPACRSPACAdSVER4.0SVER ~/SVER,,/Scal@Scan@TUBED4Tmpr<UserphARphCHR%phDYbigphQLcphTRphTR,qJ'8f7}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"CC+MCW1T0<3{M4Gk1? NM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? s>}J8kOAWm ;op$SqwgE6FM;v&KT_ZO~|Qg\c4bYJewcAN8[o*yaCCtjw&{A'h[}IT$ei^gs`2s*7k'}&8)TK7kx`rxP!t p7Zf_@72tX>VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8K%}jxz)>`VWYhY8|kRYN4+HmLP}6YH4HUXYnuPQh^fP%4X+*flT)FTX>EbrB%P^gWT|0\d&nPl'Z9dp"iK]|UKo9UC|T[[66`aD/ NE-b}J<25sb[!p?p5"buT&*z*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTB1PtB1PtBCTS~:BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?VTDATA ?VDATA ?VSCDATA ?VҙDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//UGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM=zhP1RL=zdP1WD=zmP2AM=zuP2BA==ztP2RL=zqPBAS==TgPBAS==R*PCON==aPCON==_PDMFTwPDMFRPLOC=z[PLOC=zVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPL&&TQSPACAdSPACRSPACAdSVER4.0SVER ~/SVER,,/Scal@Scan@TUBED4Tmpr<UserphARphCHR%phDYbigphQLcphTRphTR