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L,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|zxwurqnljhfec`_OOOGGGddd{{{   $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 78abdefa1d6e47c195dc3c0aa200a798Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-WellK1320_D11_Sibthorpia_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z%+7Kh#-8EVi{fK1kL19Y}0b/F\rpK(jYPMJB4~_>}tmf`[VROMLJGEB?>=<:71+$ !$*2;FR^k{2m%_04%c#a%qaTJB=;<AJYoA+U)EH6j3/`G2z-7N2Kzv@/oM4#$''%!  !'*)'$#$(-39?IYs]R6C[;%")-02356640)!    !      %Z<e)H3{Q0n7  !!     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C`MbI0'T3<( 9V/HERVM)!XL>?LiJ#08x1-yMklT) m\Fm2;/I,1WVun ,FH=| 9:dHCA[Y4Ju,N8w8mCZ%S N:qp.z{%qF/S_7{`%hb"AEQ^Rl*ktIHoI+&nQz@kv3 D) ` (O~;jD,b`]LSXnuU8)O=L-6ri&7$$:i+0^F ) ss=vzKQOTr6[(R0#72=sqB$R/-/ Z 6 lHYt<z#P,0ffhGTwH`bi&fZ0DFs!$8uFa ,6#n3F ~a lS$|$&eU`mr7[yw|}`rk[b_jbetUnKtONZS<WV[Q'/2U/;(B=AB68=3'&4(238/!%:48*4,33&)',2>!'((81!"*808$!3 "!78"."!#!" %"&!()%&#%&& $! $"(+5#/-2**'*')&(9$%54./D>*C895: ( "*-0'#'0*8&.4% 6%%F>-%70@-5IJ3(DPPPM6/GOQFI@@@@@NPdg\QMUBJM@M9:XSP_QUEISWWWJ\9KmmJSY\[hpUVjoR]L^[^[Pdiniigwhmaxwtpcqq]`nrmwfzt~lpum}vssso}z]#wd(zu4$Qp&pkppnppsr9Y8,RI="Zdp=p,b0`8d[[3w4U8#pp5*s>pd0: p ppEpGpOc p pppV$dPpp8p?TfgW1Vx ' %zN}#@@hu+s1%4>i@*& N[i=N.%Z8]B  -?0!%!,$'EQ"' DU!2/.GDP+J ,9)$"", #/'#& )4  8(=$   _ +b~8A=$ &Q :J! ,*0)'/*"$#W '#" , $*)!&'%.""")+58'=*mp0!2T34aAls*/#2HnpL?#)7)".@<R;3'>z55 0';%P%s[-$ lgT=+n760GsOb1Ne.oV<J+/Q-=MJR8,|CWIpRcRKfc ylk)i.1SuhDVyStf 0`iz6NiiJJO7^htnx*CD"} <:vNCQEO p^\zkwHK% qpN,M@Ri$ju~X0wP}7-!S*l1 GGGATGTGGTATATCGAGGTTGTGATGACAATACCTACCCACATAATACTCGTCCGAACACGGCAACGGATACACCTATATCCTCGCCGCCATTCGAATCAATTCAGGATCCAGAGCTTCGGCAGGAGGCGCCCCCGGCGCCAGCGCCGCCGTATCCTACTATTACACAAACACGTACGTGCACAGCGGTTCACCCTTCCTTCGTTATCAACACGAAACGTGACTCCTTCGGAGAGAGCGCGGTGGAAGAGGGGGATAACGAAATAACTAATATGGCGAACATTCGAGCCATCAAACAGGAGGATGCTCTGACAACGACATGAACACCTTCGGTGTATGGTATGTAGGTATGCTCGGCGTTGCGCGCGCTCCTCCTCAAGGATATACAAGTATTTATGACGCATATAATCCCTCCCTACGACGTTTTTCTTACCGTATACGAAGTGAGAGATATGTAAAGTATGAATATCCTTCTTCTGTGGTCCTATGCAATAATTCAACGGTAGTTTTGATATTCGATCCCCACCACTGTAGAGCTGAATAAGTATGTCCTCTCTGTACCTTATAATAATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:51:31 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtrASPtrB1PtqB1PtqBCTS[BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ;ZvTDATA ;ZvDDATA ;ZvADATA ;ZvDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~Z"LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS/hP1AM=zEP1RL=zAP1WD=zJwP2AM=zSkP2BA==WP2RL=zNPBAS==1PBAS==/PCON==?FPCON=== PDMFTpPDMF/PLOC=z8PLOC=z4PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%/`SCAN@SMED SMLtSMPLTQSPACAn4SPAC/xSPACAn4SVER4.0SVER [SVER,,/Scal@Scan@TUBED11Tmpr<UserphARphCH/phDYbigphQLcphTRphTR+This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtrASPtrB1PtqB1PtqBCTS[BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ;ZvTDATA ;ZvDDATA ;ZvADATA ;ZvDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabjFVociFWO_GATCFeat//iGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~Z"LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS/hP1AM=zEP1RL=zAP1WD=zJwP2AM=zSkP2BA==WP2RL=zNPBAS==1PBAS==/PCON==?FPCON=== PDMFTpPDMF/PLOC=z8PLOC=z4PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%/`SCAN@SMED SMLtSMPLTQSPACAn4SPAC/xSPACAn4SVER4.0SVER [SVER,,/Scal@Scan@TUBED11Tmpr<UserphARphCH/phDYbigphQLcphTRphTRqk+VxnDS\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|T9}LU2mV)RmFcj`@hBFf?0p=P#`0AlBE%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa-s1tob*oCfvn}4|Md'ac_` WV|N&4MMdr Gw7[k39`(Ck.e{:^kkSOj!Q4KK*flVy8J9tGHrd+"L&d&2<\d&k@6GZ]]C)TiSOpP S!oS{Rn[-Vt2`\wmj7ar?s1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52rRU)>`V$];9'k0K3AQZ`K'H:{GKipM`{VL7f4AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtrASPtrAUDTxB1PtqB1PtqBCTS[BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ;ZvTDATA ;ZvDDATA ;ZvADATA ;ZvDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabjFVociFWO_GATCFeat//iGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~Z"LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS/hP1AM=zEP1RL=zAP1WD=zJwP2AM=zSkP2BA==WP2RL=zNPBAS==1PBAS==/PCON==?FPCON=== PDMFTpPDMF/PLOC=z8PLOC=z4PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%/`SCAN@SMED SMLtSMPLTQSPACAn4SPAC/xSPACAn4SVER4.0SVER [SVER,,/Scal@Scan@TUBED11Tmpr<UserphARphCH/phDYbigphQLcphTRphTR