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  )  # #$                  L,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|zxwurqnljhfec`_OOOGGGddd{{{   $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c1128f154d1347338f7c14c281dce66cSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-WellK1320_C2_Echinopepon_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z%8Ss%08=CIPW^dhknoqrsssrqonmnrziN2gN6! 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YC@F=a"1 U;0TY<.!R\6, %% P=LY_cL?K:/NG69:&c:H#!@1[ F(TW<,)+`-:06T^ H>E 9(K:\4&L[F7<M3 FPUCX/+@#[!"%H,99ZXAIMZE)d^,V>I'" C9U9;IJ) 0@'( 16Aa ,=MY3(CYYDWB! -,SQc6J+A^ /K 88@0I>9'4MaQ8C !/%^N^":#C8Q'S0A7NN%XH>GA#@N]cIOW2A.S=,J$=bUYC<L`Z>P[bGWE]QDD6& =D ,@\2]6 D*O142/$ SX/D^A.B4=E9>V$HD ];)2(;V)F %0U: ` J*dx<3U6ZZIXm Ejba,t~EY0l/=2)Pvlq/F0!*#{a&{{*8Pz[";U-+ -!wYEYN.}{->xFC7Z}8<2WN "t)bB &2S7'8tNG2EST< b kx1FL&N\KVTMz2T8"tDlg8 Pp(znsr#X@-CLfZe/f44ym IaJ'{,CI`r4 m|=V rZxv}Hq"h. $t/"SF]-86^] [ h9}zqf@^D. _ z08Yl8#9oJPH}z||sqxb bsUilTWPRZ WGGF>K8@;B<7257=7@<5')(-5.704:90",'6,2!& '):&&#(*!$&! $#,!#( !           $!"' !!    "# !,'(+0"*.#- $&*-./1&)/%$6 -7?+8&14+7%2&/213(/-#769:6+32*593;&@9DE=.@A463=.CQL;KL;NA@2MJOB1GBCIHMoGWm^>DSFIJYNP@KQ[xu^WZfWj}lqe~LemV`Cd_ZP\wOenVjJ[kWTr{biPZ`QqnTkYmg\]~kXc][dfcMMMMMMMpppqypggsyMMMMMggMeppr"z+jy1{ggg%uurq}-~ppbggpMMggggg5o9Drpvpap~pp"p,10"yR=^GYbH)byr}8 :81v-z{],Nr!2SW}p~D1t,i88UEe9t$pq,05{CpSpp> *UJWT1upYappSpDL=s"~p,Ops$-6pdpp5 o1w8JTfB&M h+G S' 1_F3,?0,pgHHmp%!+MMJ%((.!!"#/#*#/ME#,4-* $2%/ * ##%%/ 0$ *$",7/&: +%  HY# !! %)*)*-,&#I 7#%#-. $Q! 2 /& *&l # $#"-p(*"!%").> ( D=1!=Ac'0!B3#YQ+2_5-%)I!3"BG'"):AC$0fe+6 !";RBW%RA+T/#SX=Qc7FpJBB ,_J0^ 6-V?E9W;ZD8MM'' iAR&Xag<R6_v"Uw'T\ {q"hc<tf qw.QwXw%>|YdN*2D8lA,V6GAGATGTGTATCTCGAGGTTGTGATGATAATATTTAGACACATAATATACTTCCGTATAAGGCAACCGATACAGTTATATCGTTGCCTTCCTTCGTATCAATTCTCCATCCAGAGCTTCTTTCGAAGGAAGAACCGGCTGCAGCTCCTCCTTAACCTCCTATTGCTTGAACATTGAAGTGTTCAACGGATCGCCCAACTATCCTGATTCACATGGAAGAAGGTTCCGTCGTAGATCAGCCGGTGGCTGAGGAGGCTAACGAAATAGTTAATAAGTAGCCCCATTGACCCATCCCAAAGGAGGATGGGGCCACACCCATACGCCCACCTACCGTGTTTGTTATGTAGGTTTACTCGCCGCTCCACGCGCTACACGTCCAGAATAAACGAGTATTTCCACCGCATATGATCTCACACTACGACGTTCTTCTTTTTGTATACATGGAGGGAGATATGTAAAGCACGTATACTGTCGTTCTCTAGCCATATGCACTAATTCAACGGTAGTTATAATATTCGCTCCCTAATACTGTAGCGCTGCATAAGTATGTCTACGCTGTACCGAGTAAAATAACCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:50:26 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt=ASPt=B1Pt<B1Pt<BCTSaBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 1cTDATA 1c(DATA 1cDATA 1cXDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~ LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM?~P1RL?~#P1WD?~P2AM?~P2BA?? P2RL?~PBAS??jPBAS??+PCON??PCON??PDMFTpPDMFPLOC?~'PLOC?~PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAhSPACSPACAhSVER4.0SVER VSVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCH&phDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt=ASPt=B1Pt<B1Pt<BCTSaBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 1cTDATA 1c(DATA 1cDATA 1cXDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//|GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~ LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM?~P1RL?~#P1WD?~P2AM?~P2BA?? P2RL?~PBAS??jPBAS??+PCON??PCON??PDMFTpPDMFPLOC?~'PLOC?~PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAhSPACSPACAhSVER4.0SVER VSVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCH&phDYbigphQLcphTRphTR.iTr8p|_1{63_`EHsMT@OmAmDe8HR/D2GcA13woF$1PI?p":*Xq)apMf4S><3$G,lwd-l#frL?*Y\FX`@kjJ}B~xGH$i*8j|]]u.ibuILW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A\&C9":?ZEq1hu$#k:X=<;3On&+VN;172e,qz7|!Y<,h`Fzmj7Ul*8ml%R7QKiB_16!hNqKk(c:=Gk;0|5Q1V?|&@8ja:3AGXS<< xrTW?iMAkc PkIp?H^Poa#]}Ko)6V8JX|K2-!;9xxsWD@|DmOY,f *"49J_'JKxwQ4fC\{lKd#8 UC;2i$@jEl0$ed}.Iq/J#~gJjwJH=udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"a-cZ,f*F8B,}|O[nY?KPQh3f9b: 4zM=j5FW>[w!i?p${})dc-YO4OnnTfP_UqN^Fu*tayr=u4|8e7s.FSKpuze/M#yKA[,5SZ=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaxJK[Yl;j7V3]Hp~!U#