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  $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 590b0620c3b64cd9821aabe2e4b7ba95Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-WellK1320_B8_Calceolaria_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z%)0<Ph~-=LYbfhhjnw~jVB. |m_RF<400269<?BISas Dh(4?HPYbltz|yqeU@' 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ZR"#vfUKg~jH}Kdn Cy^9Nf.~  ;wJ@=f &&,I 82x^Xf$+7jc`XDXqMl-'H8H/E tja?n\fZ8*Aj3= dP/M><*\*kB&}_J(a~M%)kw\ZR2f+I%MF{FUCm9Qb;^ju ;}EW%Kx'W.O 0o |lPVQ:B2=WC}@Xm _+O3MNASB]hG7   HR$8,KuO0,/C"pgnlsotnyQiJpeXadrw<a_4TWIPXMZSX]WGDOG[YVCIGPE@[<0J:5@+9MH@=FA?%).$0$4&5,29<E+8&,8 6;$6=$#%0-*),+ 1=-! <700%*& $>+)( ) > )#  ( "%      !  !!1+ ( $8LE+8@C66*!'0N9B.02!0$0DD+L7;6($$2;*/8s9:<$$*nL<$$$AA',0/:3C:4J)2dO00:AP!79I00^000-00+500B:/0000000EEEEEEEEEEE>Sv#EEEE=EE71'Mf'EEEEE&EEMEER>1@PXEEEEEEEEE<9PJ9@EEEEEE2EEEEEEEEE9<M8;jEEEEcE:EE37f/EEL9IEbe@ET2E7E EEEEEEEEEEEIp ,pH(p,4Y(Xdp!xd*pd~1":HCpEbX_MTqn#{od-FpC*4cppp9p!JRppj?.\pb pFV-n!4pjppA7KM2F#u&np_fn+;4W|!0<%%Os"XT="k13UUP+(@rbL-,aG2Ys|O'1=$f' 9i]Q<S ">482@<X* W!cn #:$/72"G7^+7|c,a"vR KWB S4{M XHA rt}j4W^AV|>G/nK:qyDrl@zW`np"h>*JwkSzBl4|n4`&N^9FLeZfBJ_&;[;?h #&G="Sd|S9$wU'CASYxH.7r]{!Bt#izQ|"c[(abr).1  q .WRlLLP!>N0@e8.ejgO{,ls/><k+FXe|$\gCU+aCAL e; &.(0IzG8b;- .<@GAAAAGTGTTACTTGAGCTTGTAATGAGAACACGGCATCACATAATATACTAGTGTATCCGGGTACGGAGACAGTGGTATCGTTGTTGTAATTCTAGGCAATTCACGAAATCGAAATATGTAAGGAGGAGTAATTGTTACTAGCGCCTAAGTAAAGTACAGAAACTTGAACATTAATAAGCACAGATGCTTTAACAATCCTAGACCATGACAGGGAACGTTGTTATTGTTTCTAGAGTATGGGCGTTGAGGAATATAAGTATATATGCAATATGGCGCATTTTAAAGATATAAAAGAGGAGGATGCGGTGACAATCTTATGCCCACCTATAGTGGAAGGTATGTAGCTAACTCGGCGTTCCGTGCGCTCCATGTCAAGGATATCCGAGTATTTCTTCCCATATATGGCCACTACGACGTTTTCTATATTACGTGAAGAGAGATTGTAAAGCACGGTACGGTTTTTCTAATAATCACTATGCATAGTAAATTGATATCCCTGCCCTATTGGGGGATAAGATTCTGCGATAGATAAACAGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:51:10 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt;ASPt;B1Pt:B1Pt:BCTS BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA %K.TDATA %K.DATA %K.DATA %K.6DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE>LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AM6P1RL6[P1WD6P2AM63P2BAiP2RL6PBASPBASPCON@PCON%PDMFTpPDMFgPLOC6PLOC6PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%HSCAN@SMED SMLtSMPLTQSPACMSPAC`SPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEB8Tmpr<UserphARphCH~phDYbigphQLcphTRphTR"IThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt;ASPt;B1Pt:B1Pt:BCTS BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA %K.TDATA %K.DATA %K.DATA %K.6DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabhFVocWFWO_GATCFeat//(GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE>LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AM6P1RL6[P1WD6P2AM63P2BAiP2RL6PBASPBASPCON@PCON%PDMFTpPDMFgPLOC6PLOC6PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%HSCAN@SMED SMLtSMPLTQSPACMSPAC`SPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEB8Tmpr<UserphARphCH~phDYbigphQLcphTRphTRXPvc.[.u2Zv&1x5roz;P= =&e=1&!rE_g4Y@D}4r(>z1{zK90plkm$48v3}k76gg`q:QS#tyYZ(WxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]3Fo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEey069>R/Ury&EHtVeXciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u $O6%nOp$lYTPSD`^BnBCmJ1y~I,YtR%g`+m6L#BlHz24a^jmJ!]`-;z128)TK7BKv'xPP"F77jD8z{V-AMV^@Yo]p&9'*H7C[%`f$LRuo\`.LH|f{mbzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:#a92!ap(Z~~gln:tl|3[!]"oB/mn`kI Aq@E4/wL@,Jy$pd/_GPvq6;1&&d]wv}BSnaN;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#ky6Ka;v8e7s]jTj.uFn4o[|.]/E7hlC+}NU82vS3tM,M|n921kMYYH|O[nY?KPQh^k 1{sI(J8dM!tqw8&rd+'n,J6v$NU,l9cGY@ZWeo)GjL~7L3"6%4y^.N82`\wmj7-U.*P-*KWeq }X'K2MWhc'kT'_C:rF(5,(?z7b[z`>F3!sY#+~/]m#NA^6h9e *E&]E64Ut.K5b TZ$Y~8gyh/Et8gw'JQEhXBN4UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISPP1AM6P1RL6[P1WD6P2AM63P2BAiP2RL6PBASPBASPCON@PCON%PDMFTpPDMFgPLOC6PLOC6PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%HSCAN@SMED SMLtSMPLTQSPACMSPAC`SPACMSVER4.0SVER SVER,,/Scal@Scan@TUBEB8Tmpr<UserphARphCH~phDYbigphQLcphTRphTR