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  $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 843347a991cb4831b91ba69408f5500fSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-WellK1320_B4_Alnus_acuminata_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z'-8Kh/ANX^chlprrrrtyjK'cG- 4Sw 0]DdrU<(xcRD8- 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*((((!   %(  MS;W#'dS 3P %b/(Y:/" +8UE\3>SQTM<50\D=/'EP >]3$:V[` ;T\R @BM=`47V4)?A 3=89>#.!<QP@"8dZO3 D112H4%' 3] =%T(@!6^2!P<,6Od[*!,AQD#-25 3 .[ &L9"a=.Z33/C2?/#: 3D"F[Y@*6I?c58LBa@-U-C"1F]HJKQ1@OY'4%Y 62Hd`BCJ!H O(C-7"\2GMJV:IB/3<8\N0LJJ"MSL7B+R<WN$\'TFOd-O?L( A>I"A%1=\1, U?_->C23EX D[&)9S$'(4P] U& Q#Z9R9H^#=\aAT6(@6?:Q IH; ]ZXZUc<.O(3 F.RKY-\"9:_&DXbA3N8bQ+HE2U*V)M"\ 12/YNU6>7]S=\AYW7>X!\JKO>*D/`K05XC0Ad F&* VOEO@'SO9W <G3QRC'R2TS$73>C}?m:h5_n=]Lq( wFzIL*Oznk  'H?OLd^IRP/M BtyqJ~OBEPTX<AA3OQoH} hF!yPd~5'JZ'2*ist /[-fWms"sneF1pp 1a3O:T+yb_ Jb/.tno)aeX:"c9&LMW "Z!4D ~fvtU %{Ck- % %;xCZAeOP3j.sehun 6%a'r{O-qQ LGJ`_.(6(}a&=4H|DR2GGx-Z^{xhH?Pl|  ql>(PKP:nrI%vQ ([F=|rrrs[^tdjgagfReVHcImzGQCNOJ7E>9?C523/&+,'0)3/+'2%55,*))5-&#)#!0""),, 1*#*$        #$  # $!) !" +)'(#""!,&)" !! +%)&%*&$@0$% -'!03)'9 &;?KI51*529*7$$967.8;619+'08B$5TT=0?Q:J=:C/P=D@V68;>=I>BSORRUgggSSaTDNY\`TKZG[aRVV?NUXKZ`KRP_|Wn}~Rm{V[`_dU\tlbizyp{t]y_cjc}nguloa`fmmmt*p{qet_yyaplmon}wm||rrzGrr{~~~~~rrr (~~~~~~rr)ppT {pdo/pkpJWcj>YdzdJdl|1u Vd>pM;E.G  $St8brfk>p,8 j l?Dp,pEk<p\pdp87{C+<p+p1vp x 7:hW;=p&?Y  "jMj5iPA+- 5)ei,"5Y!3Xx:')1G?!7-. +(#4R0(E6Zo'; ,Y9)-()1+>  *(!(>;"0  Fz ZN@'%/ &%")%$ -H/!*'%*!',!"%% +)!! *!"&#"7* !4L)!,,'/3*!#+d5#/ !:9>2" 2 C3Cs(11=^|$3u.J'.<932Z)PH6gK2"(-D/'j'VW,b0@'19Ef7e8ELn=UJW>U,-GI907CMSM%=YYZl3=0(<D5P8['?LI,A"JI<QWSYTZm("+hUCML,VXIeNo_zX6v^jlfd{ ,x|0w^XUpEW`^ v$GAAATTGTATCTCGAGGTTGTGATTGATAATATGTAAACACATAATATACTGCCCAATACGGCAACCGATACAGCTATATCCTTGCCGCGCTTCGAATCACTTCACCATCCCGAGCGTCGTCCGAAGGAGGAATCGCCGCAAGCGCCGCCGGAACCTCCACAACCACAAACACTTCCGCGTACAGCGCCACACCCTCCAGTCCTTATCCCCACGGAAGACGGCGCCATTACCTCCAGGCCGGTGCTAGAGGAGGGAGACGGAATACTGACTAAAGCGCCCATTTCAGACATCAAAAAGGAGGATGGTCCGACAAGGACATGAATACCTACAGTGAATGTTATGTAAGTATACACGACGTTCCGCCCGCTCCACCTCCAGAATAAGCGAGTATTTCTTCCGCATACTCTGGCACACTACGACGTTCTTCTTTTTATATACGAGCAGAGAGATATGTAATGCACGAATACCGTCGTTTTATACTCATATGCAATAATGCGTCTGTAGTTATAATATTCGATGAGTAACACTGTAGAGGTGAATAAGTATGTCTTCGCTGTACCTTATAAAAATAAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:50:41 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt.ASPt.B1Pt-B1Pt-BCTSt7BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA >i|TDATA >i|cDATA >i|N5DATA >i|DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~rLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISGP1AM>|^P1RL>|ZP1WD>|bP2AM>|kP2BA>>ppP2RL>|gxPBAS>>JRPBAS>>HPCON>>WPCON>>UPDMFTqPDMFGPLOC>|Q PLOC>|LPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%GSCAN@SMED SMLtSMPL TQSPACAe5SPACGSPACAe5SVER4.0SVER t,SVER,,/Scal@Scan@TUBEB4Tmpr<UserphARphCHHphDYbigphQLcphTRphTR/This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt.ASPt.B1Pt-B1Pt-BCTSt7BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA >i|TDATA >i|cDATA >i|N5DATA >i|DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//RGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~rLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISGP1AM>|^P1RL>|ZP1WD>|bP2AM>|kP2BA>>ppP2RL>|gxPBAS>>JRPBAS>>HPCON>>WPCON>>UPDMFTqPDMFGPLOC>|Q PLOC>|LPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%GSCAN@SMED SMLtSMPL TQSPACAe5SPACGSPACAe5SVER4.0SVER t,SVER,,/Scal@Scan@TUBEB4Tmpr<UserphARphCHHphDYbigphQLcphTRphTRR)U`l5j!X`7.qT*uq&6i}8Amabj1A6rd)ziA=sUOzj{>ZHm? 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