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$$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fa021145d7144724aebec848a9188445Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-Well!K1320_B1_Prunella_vulgaris_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z.Hj2J^ny  %2@LSSNF<40./26<CLWcmsusolklqvzzvnbTF9*^B*o^N@5.))+3@To 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Q[8<@ 6(H'42*<8MYc<07IA/,;&3', !'((OUTE.W68B5%F-1"]@"MC[B X]O;@\07[R9&@,.%\`<.9R#A*H?X83)->5H-AF4KF+^-C%[> G<*>>+'<8ME%H? 9-^<'FUFK+(BF2,PG9c J#^?-B0C1?Z2@)%3H00 = Ub)=#.^D5N!3@T#1A$%^K V"?]E!%H?LNS8$' 9 K)ECS\DM:c+F&7Q%5814T)>7b&YLS^UU)9,!QG'G.^A+BA1]' Z #L$)5`##M5-UF+/%(H 8M0; AZ0ca/5"X@?_7QWXXR* (JD(<*W%%48H:G8B\' QN ,))aP)+0QYAY[#_E,@Q2Q& 2QO YL`C8>X^ ZQR -5?OCM4E@#E>G(D"512 />K _2J2AJO2^K6A !'$V@X8p2(LOK* ZAa: )OWplq/nfo j!lhq 8~4T @x& n| L _5 p\Jjid-{tfB'-=AKQx4O]+|4S3zn424|jtI la`!*@<|eYgSgI8%i/b*I)|EF2P^_?n7JfH)@zfG4Vkx(H@zjM<,ecAQV q'V7cK%ywo ,3$Y$}FJ I(n}M.zl5'fOAr9ta/X9 v1!`P.PoN5~[AU+}@KCInK_u8_SdL{]8ya u`#0Xm/ 508  J r}fF]!F,DHw{IwznqohpplldbkwSZtafWQTFQ~oMa/64<PQQEVFXHDH;K?VKE6B99W2'8@=:6&27257*558" C/H0C/L E)8$ -((.%-2&"(2##$/*"(& )* ,!,-! #0-    #&&)%##/$ %(&$#%$(  ($ #//)( .%+*.-".4& '/!)37 '/=*.M1)(7D"$5)20/+5H1&K?6$G381-CD:0A8%O@$,1C;9O'2!/=QAAER:JH?(C85(+<2T|{C[VgX\o99tY~{g3_V`H>E*#AcfTPYYfB:2(FD'0;]l#>qZ1B0DQZA6M**Y=FOUBfq:78=FI%P???**H???AA777NNGbBLT??????TwbB77c?`KH*77AAAh8?AXcAA8?^6Ol\A?7??AAA?'1pppAGVlpKp(.?dpp/py4p$^p l"; qMHDN7\kc @w<%w?i_so=#fly|z\TCJ(,75ujDs%<!Pf;|~x/[?hDr!8}%#< t*!}< ~rx?"0t%$~ib sx78H-zL0^sSlyzz`v$f*."E]aZTf[QaC`X|Gt}Pd;:7-)UBqWY~4iT@=~>Q5sUw<BZ$YZaF}M|)Q]>rYD'YLrQtmwcg;F=po8w 9}hI-^Z_u%&cist)]}H50bJ eB|< ;azdhf V1vp6vYAwq[MS'`62!'gRZFmK%AH9I8r @GAAGGAAGTGTTTTCTCGATAAAGTAATGTGAACATTTGAACACCTCGTATGAGAAACCATAATTCATGGTATATTGTCCGGAGGCCGGATTACTTCTCACCACGGAGTTTGGCCTTAAGAAGGAAGGACCAGGCTACGTTGCCAGTTCTTTACTGGCAAATGGACACATGTATGGGACCATGAACTTACCATCACCGATCCTTACATAGAGCGATGCTACGACATTGCGTCTGGCCCAGATCAATAAATCTGTTTTATAGCTATCCCTGCAGGCCCTTTAGCAGAAGGTTCTGAAACAAGGATGTTGAACTCCCAAGGAGGAAAAGGAATTCGAAGCAATGTATGTAGGACTACACCTCGGGGCAAACTGGCCATATCTCGTATATACATTTAAATCTTCCGAGTATAGCCTCAAGGGACCCAGGTCGTATTAAATTAATCGTGAAGATACATACGTACACTAGCTGTAATGTTGTTCTCCGCTAATATGCAATAATCCGTCTGTAACTATAATATACGCGGTTCACTAATGTAGCGCTGGATAATATGTAAACCATGAACCTAGTAAACAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:50:27 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA +WpTDATA +WpDATA +WpsDATA +WpZDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~;LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS[P1AM?~P1RL?~ĆP1WD?~͂P2AM?~~P2BA??P2RL?~PBAS??PBAS??PCON??GPCON??PDMFTsPDMFrPLOC?~PLOC?~ PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%SSCAN@SMED SMLtSMPL""TQSPACAjSPACkSPACAjSVER4.0SVER ޹SVER,,/Scal@Scan@TUBEB1Tmpr<UserphARphCHphDYbigphQLcphTRphTR$This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA +WpTDATA +WpDATA +WpsDATA +WpZDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~;LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS[P1AM?~P1RL?~ĆP1WD?~͂P2AM?~~P2BA??P2RL?~PBAS??PBAS??PCON??GPCON??PDMFTsPDMFrPLOC?~PLOC?~ PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%SSCAN@SMED SMLtSMPL""TQSPACAjSPACkSPACAjSVER4.0SVER ޹SVER,,/Scal@Scan@TUBEB1Tmpr<UserphARphCHphDYbigphQLcphTRphTRk&zt Mi3rs{6`V5r@AKl&FaewMZ>fcn.=[|ebhktH4*&qve4q,8kf RCjxeCW?R5Ch2d'jw~|>J3zFReL#(78WFoe~8/zz-nX>z3KQv3.5s\yC!}WQ`Q5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ$zWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCo6T*-dotq$C9|f|Qc^F"P95Li2R~5OPh~jThh@<}9W#()P0&Mqzva].p>x'[vA-\K"L1{!Ul62dl=?o'%:`Kle8h/_Dea_';_/d@ikjFDfqcd o;X.>S[jWcgnW6Ob#7!VkWtt/Lq<1j+_FT:)+'2|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9]CR"lWW .C&q6X+9muy%lFLuLGtrwEbIWL/=z+9hJ{w397hVo+&k=;A{%i|o(>V&=o>Z$~y;|>,m_noM$mOYePWz"$QAjat>9.b':8 v=iqNs)lktayr=u46_n&)-%Tw1(_CcSb;6AI8rBAr `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gX