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  $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8e4b95b46d0447078f733f2a5e756e90Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-Well$K1320_B11_Amaranthus_spinosus_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z);HOSY`ir|  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"            N18`"Md(cG6K%JUZ*G-&+%`NK RCa+TNd`1W*\P !]8[ 1,U0\<,H6*PIRV'UMG#$0#!;F;% dc<SU,:V=JL+`B1717X(GANROO=(?G3<"<6=9c=_]2 C5=$4ZK>#Y2WcF_@cJ ^<<K24\9 ?7)'JED5 0:E &I17;ATO$E*%&V8!XW::#9!F2$6IZId^+`GN.$,%YGU7dV=U,"):NYW;S4IKOC/A=I6)CYZ2P99=\2OLJa9M+& B: ;TaRE7%N7bR/_] 6UPbL /#\=c Y+.27@ G[P54CBDO>MD(EVHT&^4J`E$6AXAIF) I&+U8G+O+La\X J;:OT++#SU5/8 :bE!X<`(b"+?`V2U+Ob2FUb#b!CO &1D12<A<.BPA2%*6b29b24JhX P \[th>9KDM M-gFagxA,KbtFEERKrRgRMl2Qh4y\h Q-e+chT7w@+DN(Kiwi2#)7 M!vc\@K:[5J^ `)Fa$9`b&7TU _ pGaqH~[=~}NXN<:W9bD pEBF=f&(>&gC<1W \Sd8 ac CMN0whK9,VJ sz^<MBDF@R82iIP'n7o^] u  vM2FZA-jG<_MxduA Szb.w8}$ ;:u}X~];\F+"Sdt/mX]NsRrPhHF8,\`0YJFPTVULiM<DaJDTLN$5EJBID)Q1469$4:0.6K:=6.  /$ '! $4& "# !#$% !   !$                  $&  (22       +E  *& -!&+",J,)N  $ SK>@DyC/L@DP#RTaZdwnd GB&**n@Xs**$******!&**!****'*** ************,**3****.r10)+*/*  /*(#*&* *********3pR%d.&JY !"pd6,pOdppd%,Pp)&zIp,^~Rapappppu-p>,S-(wPp?v}pFt:0V8h(lpZm*&P ="z fv)=R,'i^4>\,x6NQ,ju );1C4B=]"+$ $)%- +1,'A2G6,"2. NQN8#:1>KC:! #+!%'!9!(!'' Ga';m7 >r"5ad*)"I$TU4_S*# $$.40&B=U&B/HB' 6*$+) d3U2`SA/o"-'Q[!EC8]"^K5<XN/N$OrUl#, h6 R!["uzq<#<m0/zz  B1ASJTh (EeJ.HTV=9} X,77vj "7:`ST$^j~'\L/!*C#X VRB2dm&p^UtG!AGAAAGTGTTATTATTAGGTCGTTAATGATAATAGCGGGTCACATAATATACTGGTGAATATGGCAAACGAGACACCGATATCGTCGCAGCACTTCGAAGGACTTCTCCGGGTCGAACTACGGGCGGAGGAGCCCTCGGCGGAAGAGCCGGCGGAACCTCTTCCTACACAAACACGAACGTACCCAGCCGCTCAACCTACGATCCTTATCGACACGGAACAAGACGCCACCACATCGCGTGTGCCGGCAGAGGAGGGTAACTATATAAATGGTATGGCGTGTATTCAAGACATCAAAAAGGAGGATGTCTGACACTCATATGAACACCTACACTGAATGTCATGTAAATATGCACGGCGCCCCGTGCGCTCCATGAAAAGGATAACGAGATTTCTGCCGCATATGACGCTCACTAGACTTCTCTCATTTATACAGAAGGAGAATGAAAGCACGGCACTTTTTTTTACAACCCTAATCAACGAATATATTTCCTCGCCACGTGGAGTAGATTCTGTGACAGATAAACABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:51:34 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPthASPthB1PtgB1PtgBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA #GTDATA #G~DATA #GfDATA #G,NDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISt>P1AMP1RLP1WD%P2AMaP2BAP2RLCPBASvPBAStqPCONPCONPDMFTvPDMFtUPLOC|PLOCxPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%t6SCAN@SMED SMLtSMPL%%TQSPACMSPACtNSPACMSVER4.0SVER  SVER,,/Scal@Scan@TUBEB11Tmpr<UserphARphCHtlphDYbigphQLcphTRphTR*uThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPthASPthB1PtgB1PtgBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA #GTDATA #G~DATA #GfDATA #G,NDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabrFVocaFWO_GATCFeat//2GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE]LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISt>P1AMP1RLP1WD%P2AMaP2BAP2RLCPBASvPBAStqPCONPCONPDMFTvPDMFtUPLOC|PLOCxPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N%t6SCAN@SMED SMLtSMPL%%TQSPACMSPACtNSPACMSVER4.0SVER  SVER,,/Scal@Scan@TUBEB11Tmpr<UserphARphCHtlphDYbigphQLcphTRphTR"3Z2VW)$>ZHm? 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