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L,,,III^^^{{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|zxwurqnljhfec`_OOOGGGddd{{{   $$$,,,444<<<DDDKKKRRRYYY``_ffjllrsrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: accd299e1e064b51beee2247933b40a4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_22-03_07383130Seq16800134881_134881mfagan96-WellK1320_A5_Ageratum_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z5>Oj 0Kdv  "! )7EQZ^_^^`fnuzzvog^SG6!hDr`N=/&!!&/<Of-W/d(@QUI/ lW@& xX:wR/zxvvtroljhhijmnnlid^YTPLIEA<7410147;>@AAA@ADKWg{'T#LmqHsC~wronmoqw$aSo9SVCY8''8Y7C:J1K>9q8v!ANx!y[F80,++,,,+)'%$###!!&*.123459BQhSX\ R{ZC5-)&%$%(.49;:86421/+'"  #%%"  %(*****+,,,+)'%$$#"! 5`u*.&oYX2 !##"            !$%%# .ObC/V>j( e;%#(-14676654344432/,(%""%&%"0XaH-PX/ !$'()(&$" !#%'(((()*-0257751+$ "%),02442.(#!%5WF<"h&M3eT) &/8?EHIGB<4,% $(+,,,-...-+($  7U KN!&*.0/+'$!!!""       &ox1 >BFrtNs$FC"')('$" ,Rw22i|7 #&''&#!  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';Z '2-yU:&%9W~bC,     $Ah%#nJ. ,Gl#@MJ: :Q[T?   `G4&  *EfhF,  *CcmL2   1KitV<'   #6Qs<T_YCiJ4& .Ef+::-2BD5uV>-!"4Oq "(lM4#!0DYovcP?1%   2Lm  lK0 $8QokN6#   ,@Yy dH2#"*7Kf*3/"!.2,"-0*  ! cG2#    +>VotZB.  ,=RiuV<'  "0BYrqXC3(!!'3DYstX@-  %/<Nbz~eM8( +:Nd}bH3"  -=Pf|nU?-  "1CUgxlXD3%%2CWn|aJ6'"+6ALV^cec^TI=1% -FdgD'  !$'+18AM[l<ZryeL- m^RH@92,'" l@jA&AJg8A =KB.bcpKB_3730_POP7_BDTv3.mobtermAAGGTATGGATCAAGCTGGTGTTAAGATTACAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTTCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCCGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGCCGATGCTATGGAATCGAGCCTGTTCCTGGAGAAGACAATCAATATATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATCCCTATTGCGTATATTAAAACTTTCGAGGGTCCGCCTCACGGTATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAATTTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGCGGTGGATTGGATTTTACAAGGTATGGATCAAGCTGGTGTTAAGATTACAAATTGACTTATTATACTCCTGAATATGAAACCAAGGATACTGATATCTTGGCAGCATTTCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGCCGCCGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTATGGACCGATGGACTTACGAGCCTTGATCGTTACAAAGGCCGATGCTATGGAATCGAGCCTGTTCCTGGAGAAGACAATCAATATATTGCTTATGTAGCTTACCCATTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGGTTCAAAGCCCTGCGTGCTCTACGTCTGGAAGATTTGCGAATCCCTATTGCGTATATTAAAACTTTCGAGGGTCCGCCTCACGGTATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTGTTGGGATGTACTATTAAACCTAATTTGGGGTTATCCGCTAAAAACTACGGTAGAGCTTGTTATGAATGTCTTCGCGGTGGATTGGATTTTAC"6=W`l}#0>IWfv !/:FT`kw'3=JTan{+9EP\ht,9COZgt".9FQ\gs .9EQ^kw )5BO]gr~    * 6 C Q [ g t     ) 6 A L V b n z  $ / 9 E S _ k v    * 5 ? 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')#   '  bHQ %5TY8 2TK I S\63-/%?P;"!B V +*2.I)?[^ 5=2`+;H(IP' ,:dC["&G, GPZ'b8;BXFRVD H0ScU6#b>E9 0\=!$8@9>^468Y;XW<'?!98-!D6MZ7&D^K5?RON 5;7^DJP(Y[b26b,_>+,R 8 KBE/27> B16*J=MR#.'>X5!>S:A V("N_<@5T 78AT?,; Nb<]B#I?YQ!MX&@A _`/0.F$ B6]] W;-KH/\]5H2K .'VSPW3/U:"b$` ]C/)Z*/TR$ dZ>/[Z'LR:C=T!E8 ,>7ITMRDQ3S1WGOY dH.DC&J):P&KF$F9?V5F AWEN.F/:N+3H;^9#1SJ/ QL+Q*FaIZU&8>8#*$J5(L )?&9_`6P0M2`&)1/)JO^b#^`>3?!@Y9\@^L+b^  1#\*T-]At`(P~a=B+XicOWDTQZ4tfD~=US0>=oI@./% L*l-LO*Ag,a4,2i7V;|9f.o jJaRpl+ai8m>.D+U8M(Y$b (omiJ?`oL}"9GpmW{|x9D<bmO,Vp3)z0 /AKAdPK~n]&K$>&X5n,dWiG.:$+=\06wm`M.!43[T)3jg&i(Hqy7RZD*n5!?m'6@ggL$i{ <xoN_zC"1_P?QUu{W a%F'd\(f9rh:-8N  P@@H H!N]D-~L9`~wsfiqwxbl{RqXlNaqmANQTCQNACG:+E=?35%#/9"2@1$3(652%$%%1(1'&&11(%##.+( 9!%   "!  "&#( "! #+%!(  %$.!#$&#$3!#-1*)4$%'((*$'0!++''25,*-*3?6)$J:79D"")(2;SVIPCCJ.4;50++>E4LLBG>CFFFDH%IUY=?EXBHXUE1W@ZPU>JHKWRFXUbW^oqZLecMMbU^ULsQXdPgK_s]]`zfL^Xi`_etwhe\^Z^x____onfusojv\toqosmyUjOvp|{O{~uZl{kzbgu)t9 `(}=YGppe@lJ,V xX4|Ay9k6w8pu8$Gm,58U1<8o;{33pTVp+Z)pp-oCpd-pkZ8Hp3p%Tpppp)jp{/IVpvppppYICcRBC6!h"E{ .ieWNgcA,46('_78i& NO+)<"1 "') "%&883H'H'+^B),4+ $2"!$" 1$% .$*<8$$+'#%/ #%/&%8#1!$ 7'-!/ 4,.8H%H','2'"?5%!%+&+!3%;"%*.+2A6 >t +#%!%1,)4&$,C!)b%&>A,&(]:?ABT+F4?) ,Ei5L-< D/XJ62*`v+#8280S\D1wAt&a&[p#]a-96Ln,mg6?daX<F,&5OEF~Y!}o42;8h@h1JKj%I~}-PI`hL4jbb~Qxd`$]k_Oj[BHS#f'le1hVNCbhC#)$: uF P:I4!Gx_; ,g={DmNQbUf![v+]<}:GGATGTGTTTCTCGCGGTTGTGATGATAACACCTACCCACATAATACACGGCCGTATAAGGCAACGAATACAACTATATCGCCGGCGGGACTAGAAGCAATTCACCATCCAGAACTTCGAAAGGAGGCGGCAACGGCTGTAGATCCTGATTCTCCTCCTGAAACATGAACACCTCCGGGCACGGAGGCACACCCAAGATTGCATAGTACCACGGAAGGATATTATGAAGTATAGCTTGCCCTTGAAGAGGACACCAAGGAGATAGCGAATAAGGCGAACATACAAGAGATAAACAAGGAGGATGCGGTGACAACCATATGCACACCTACAGTAGATGTGATGTAGGTATGCACGGCGTTCCGTGCGCTCCATCTGTAGGATAAACGAGTATTTACACCGTGTATAAGGGCACCCTAGACTGTTCTTCTTATTGTATACGTGGAGAGAGATATTTAAAGCACGTGTATTGGTCTTTTCTGGTAATATGCAATAATGCATATGAGGTTTTGATATGCGATCCCCTACACTGGAGCGCGTGATAAGTATGTCCTTTCTGTACCGTATAAAACABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 23:50:55 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTS8BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 7nTDATA 7nDATA 7n0DATA 7nDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~7LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS P1AM:t#P1RL:tP1WD:t'P2AM:t0pP2BA::4P2RL:t+PBAS:: PBAS:: PCON::fPCON::,PDMFTmPDMF PLOC:tPLOC:tDPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N% SCAN@SMED SMLtSMPLTQSPACAgySPAC SPACAgySVER4.0SVER 8SVER,,/Scal@Scan@TUBEA5Tmpr<UserphARphCH phDYbigphQLcphTRphTR'This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTS8BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 7nTDATA 7nDATA 7n0DATA 7nDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabGFVocFFWO_GATCFeat//FGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE/LAST~~7LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS P1AM:t#P1RL:tP1WD:t'P2AM:t0pP2BA::4P2RL:t+PBAS:: PBAS:: PCON::fPCON::,PDMFTmPDMF PLOC:tPLOC:tDPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT (RUNT 2RUNT +RUNT 2Rate 0RunN""J:S/N% SCAN@SMED SMLtSMPLTQSPACAgySPAC SPACAgySVER4.0SVER 8SVER,,/Scal@Scan@TUBEA5Tmpr<UserphARphCH phDYbigphQLcphTRphTRGP58Jr7y!{_a1z{,c]n{uH<6#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`U*{Zq9FyhCebT8Poo=[/19~{2A~&!sR(&^iCq=dGE^E:DH6[~=7?xh?'kT'_C:q|4G,fX-N&Wv'i2{M~V\7%4?A8z3BFM+@bt+V8!0.9,(B~bjOe:=KKR"\3|-bWF'zP}6Ja!8UY59D4;$'bo%AM&Z'K7rtlqaxkabC;Z6Ob"1{7"{2A2{UZBwE$X~}:>J5*m#mfXr9I&YD8J-o XS_9zH'zeE^O{U,HB*@Yy-4$&+VFl@i2+`'Mvf0iGo!P#`gD AfEQ.C{+8nMHh7L6M~~hY8|kRYN4+0T{!XHTf{fzn V'o8l3XVO!DZNuOUfFhyjE^STt4)m\VD"tO(Fr~o[|!/EZdwYc_`0t;L,p$^=5NDzU4o-vNIG>S[jWcg;Z6Ob:``?(GIMfi83m ;op$SqwgE6FM;v&KT_ZO~|Qg\c4bY5Jwc q;Ix;kOZxDj.6BCeR5Qub^ucF=W.X}=}F)h>S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0