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M~|zxvusqomjigeca_];OO GGGddd{{{   $$$,,,444<<<DCDKKKRRRYY[``egflllsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b85903f4b46541fcbbb540a5d3ecc74aSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_14-53_07343130Seq16796134879_134879mfagan96-WellK1319_H7_Persicaria_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z"$)4DVix |l[K=0%  $Fo 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9UokO4A~\Cq/3 oFo[I<\z[Ql  p-Sc9&tp WCSu\] U>TAf 2[Kgnv!@ /K@::  2n[;A't\V;`!0Bjh/R~G>-+B5WqBbXLg#zP]f3S}nm|Dx, ~XR8 $ U LkMm= `$saqlU]pR]XVHapSSeYYHZZLS`PH^XD+>H2#/>>$("2>A;94;/3@G4'$32541(=4==QG<?"7;31!1,TO8;,'&37+4574>5>.5D;=)*3,>,3%(*+0(3,($&(7;3>J.74*-;55>11RI:,3(-*&."&590($+%),/-B,.%"6>:*$1('%47,383*%)*/3"",RE#.84/)/-/"-.A$/%14*-C>//!Q1i,25$>1(/()(*A0,61?GFZ@OC8/,&?9C'MA$8;888/D?EB&9NH5'/5:QQ>D07:Z*::5?<,)M(><<I:.J=46RPN;&PHB5=-<ZL+@17KHE9H]RPl+<EN5I=@QC8/+FcHRGGndod68CcNDOKLG3B@JitfPVa=3M\VV9<[N=Q}d;_UR1NNNIJKD$prcBYJVb][[^u`asp~]x{soCNHI_}YXehVKMdk}[[[kkkpUckY[[^wjRukkku[[eYfRlA/lci?kkk===;[[j[K9paE<@EqT A],Cp$3 `,}u,,SZ+ |+Ywpdx4Vp,/tpUq`ipp(p,ipGiQppoppp p,pappF)pa'ACp- Ga ex}a3c5w9Ed->:M"N/ @{ZbA'p+EU $E&5*"M2P"&U:&/$WQ#+V)#GA(b? !G5AI2<"0#25O"6C($ 1ca*Q-(#%$ $*<4508-:+b3/e-102&SJ/)$*,)'B!-"E+4)#%'&($%"3(;:'7v_V=.5i:#$D!@!'$2(9G0RH7>M'%/5!J:*/U<-q8)UK'25#<.YVG@KT>(R/5,:!;8(uL1HIFS$-I>(CDF86AP$5z/(`8;3W@K\/'J=;.n]*RN$8TT?MC7ELg`{PLs/Y78N#UmK6MYt]%u!]@ +tN$ao%+5X/O+1sm/OUNFGVGJS6s}CwOp;;[u~[`qbOb /4l-3*f$IOe.<veE$Md;@g1v1} GAAAAATTAAGTTCTCCACAGACTGATAGAACACCATTACTGGATTTGCATTAGAACACCGAACCGGCTATCACCTATATAGGTGCATTAAAACTAGGACACGAACACTAGAGAGTGATCCTCGTAGAATTTTCCCCTCGGAGGCGGTTGATTTAAAATCTTCTAAAACGGAAAGAGAGGGCGGATCTGGGCCCAAACTTCCTCCACATAGACGGTAGGTGTGCTGGTGATTACCACCCCATCCTCAAGGGGGATGGGGGAACTGTGCGACATAGGCATTATATAGTTACCCTCCTCTACAGCCACCCGCTTTACTTAGGCGTATTCTTCCGTATCAATAAGGAAGGTTGGGACTACAGCCCTACACACTGGTGTACATGCAACAAGAGGATAATCCGGAGACAGCGCCGTAAGCGCCTCCTGTTGTTTTTCTTTTTGGAGAAGTGACACTAGCTACGCCGACGATATATGATGCGTGTAATGCTTACTGACAAGAGTATTAGTACACTACATACTCTCCATCACAACCGCAGAGTATACCACACGCGCAATTGTCTCTGAATAATGACATAACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 16:41:12 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtPASPtPB1PtOB1PtOBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 0a|TDATA 0a| DATA 0a|DATA 0a|yDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~gLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISڇP1AM?~0P1RL?~P1WD?~P2AM?~P2BA??(P2RL?~,PBAS??PBAS??ںPCON??sPCON??4PDMFToPDMFڞPLOC?~PLOC?~8PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAguSPACڗSPACAguSVER4.0SVER SVER,,/Scal@Scan@TUBEH7Tmpr<UserphARphCHڵphDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtPASPtPB1PtOB1PtOBCTSBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 0a|TDATA 0a| DATA 0a|DATA 0a|yDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabKFVoc:FWO_GATCFeat// GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~gLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISڇP1AM?~0P1RL?~P1WD?~P2AM?~P2BA??(P2RL?~,PBAS??PBAS??ںPCON??sPCON??4PDMFToPDMFڞPLOC?~PLOC?~8PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAguSPACڗSPACAguSVER4.0SVER SVER,,/Scal@Scan@TUBEH7Tmpr<UserphARphCHڵphDYbigphQLcphTRphTR~WhC0-#JpPJwiO*$]_`#?Z9rS}G|u8 ]qU(6ji,x5?h8]Z~m$F5:U~2C 4"])|JjJDeV2E+qmj4I73wJzO`P{ e,&o.]/+M;`8hrjuO*@NzRoB+ks9?OkZKUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^M4'Uw\Pcc{\A_}$A>AR5Z(b|U2mC!KC_KdrWS[jWcgaWmUbe\W'KFkaqQltX[3>>B~:^gWiD!}%`8p3cma1yM\99UEmL/(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i6ti#O7(V6[/3-6)SE8pT/@MOsijd(DUfb0b0~!'> JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#ky6Ka;v8e7s]jTj.uFn4o[|.]/E7hlC+}Ni12WS3t{,; G921kMYYH|O[nY?KPQh^k 1{sI(J8dM!tqw8&rd+'n,J6v$NU,l9cGY@ZWeo)GjL~7L3"6%4y^.N82`\wmj7-U.*P-*KWeq }X'K2MWhc'kT'_C:rF(5,(?z7b[z`>F3!sY#+~/]m#NA^6h9e *E&]E64Ut.K6[ bn$Fq;,``y?25D0V31K}i>ZI>N:,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS