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$$$,,,444<<<DCDKKKRRRYY[``egflllsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 61885172ada24e03840cf397c1e8c4e4Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_14-53_07343130Seq16796134879_134879mfagan96-Well$K1319_H10_Solanum_lanceolatum_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z 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" '&"(%#)1*-# +   ?B5EU84!-'6//7!4&+5;-1!''0*:/894$2*B,<6%&//;) -9295)-7,3E)713BDFQ)1526>:2:B;//60>6EUPU^EB1BID;J81F3=6OQQMDLD5KMKQJBH[>>iOGUBLDNe\5HE:@ee_G8VAMGHPOL;KrnPMZujTFEChDUG}iQnEdDqK{]dcFFgWN\v\}FFfRtfkhFFfTd;]qc^^^ XmheYm^^^"SSSSSb FFp pqq,'_p `,pc8OG!f,h,p0^Zb[@~A]Ep+26h,8vM{,epi lEeppzplpp ppJ{pLp=i!$A8,=ppZZp(ppp $JhaN/J  |VIu Zj) %"\C 0nUD# k~ C6  i.IQ77 WA  8Q,#? t / 8" " " $ W &1&"3-' *$*d$E $" %    ."-!$*$ 0%, !%(00&!$3@ <'" ![44Q0C6-l#2a $A ".:/C4#>A91(B4=5@AK+0$7.85N(.-.LC31r2#*.;i3,1$M&+`T)_E<6QSW8B@832OO8f>[]Xb2LEi\_p0#y{<_^n?=B~As=4s%]Kk\H-DcdTd^)m'2iEn9+f);.m[,'Be~FI!ir.Py[Hy#}q8p\>bO?fGAAAAATTAATCTATAGCAGAGACAATAACATATCATTACTAAATTTGGATTATTACACCTGACGACCAAACACCAACAGTGGTGCACGAAATCTCTCAAACCAAGAATAAGACGAGACGCACATCGTAGGGAAAAAGGACAGGAAGAGGATATACTCTGAGAACCTAGAGAAGGAGAGCGCGGAACTCGGAGCAAAGGGACTTAACTTCCACGGAAGGAGTTCATGGAGTTGTGAAAGTTTCCTCCACGGGGATGTAGGGAATGGGCGCCACAGGCACTATATAGTTATCCCCCTATACGATCACAATCTATACGAAACCTTCTCTTTCAGTATCAATAACGACAGATAGGACTACAGCCCTATACAGTGGTGTTCGTACAACAAGAGGATAAGACGGAGACAGCGCCGTTTGTTACTCGTCATGTATTTCTCTTTGGAGAAGAGACACTAAGTACGCCGACTATATAACAGTATTCGTTGGGCTGCACTCAAGAATATTAGGTGTCTAGGTGCACTCAATCACAACCTCAAAGTATACCACACGCTCAATTCTCTCTGGATTAATGACATAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 16:41:26 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtLASPtLB1PtKB1PtKBCTS BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 1/b^TDATA 1/b^DATA 1/b^RDATA 1/b^{DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~ LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM@P1RL@IP1WD@IP2AM@IP2BA@@P2RL@PBAS@@PBAS@@IPCON@@ PCON@@PDMFTvPDMF-PLOC@IPLOC@PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPL%%TQSPACAhSPAC&SPACAhSVER4.0SVER  SVER,,/Scal@Scan@TUBEH10Tmpr<UserphARphCHDphDYbigphQLcphTRphTR4This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtLASPtLB1PtKB1PtKBCTS BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 1/b^TDATA 1/b^DATA 1/b^RDATA 1/b^{DCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~ LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM@P1RL@IP1WD@IP2AM@IP2BA@@P2RL@PBAS@@PBAS@@IPCON@@ PCON@@PDMFTvPDMF-PLOC@IPLOC@PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPL%%TQSPACAhSPAC&SPACAhSVER4.0SVER  SVER,,/Scal@Scan@TUBEH10Tmpr<UserphARphCHDphDYbigphQLcphTRphTRRz0L/kbnE!0z^n3HVQQEO2astcZ~~VGC2lB)lW+Uf066}ZC__cIBM}.z zFef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`P|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTveurDmCq?`DQ`+}{Uplql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W@Vk1$#=4ZbZ4yM=VrTH&zqrua*b iq*#7rO/adu{>(Jt6qZ><}P?[NB@vulc*{w@p;#(":?S`,j.uFTcM#yKA#2rBA++m}Il4N&W|1d$fnwHx*~9HB (_e:RJ4ir$G(~}d M]f)C^DZ=<7:cmj^k-T0M5[S*.JpxcSX`"lef5U_9}|Ate1}1"/bW>y&bC]LVn!<|'k0|3.B+0Oj!Q4X+*flaO8JB6E$} {r*i`CS[jWcgnW6Ob#7!VkWtt/Lqi=jAMFTx)PU&|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_p"}$&}0eq7A]T,.] 2,\aAB9*(K#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OL