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(:(586QB?ODZJKSM]b-c'9=# ~="<qAd%^ocR;P05-=/0-2$(..;3*    # 49DHEBO8?]ZU`_u Es*&& l</Z Bv{f\]?9I4VQg6"8I99MnTSH<(+%    $!&+62=/!-44+$+0% <,8&0''&5<FZMZwXu;nozcaK=IF5Zw|cDTT\:;:B7<9GH?AazKjySk<Xi`5hN<4-- 0)8:25$:5]bj$MnbwTL7+   )"0('-%*2($/$* "(*$259AP8jaXyn}x%G/Mf>d#&Vq+a~<oPD&)       $%$M[]jy@~d6"yh=>: =.2'/=ID1F6-;,($")$2-  "n,,,III^^g{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR M~|zxvusqomjigeca_];OO GGGddd{{{   $$$,,,444<<<DCDKKKRRRYY[``egflllsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9335d553653946d4ad17fc9c082a9079Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_14-53_07343130Seq16796134879_134879mfagan96-WellK1319_G6_Hyptis_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z .:@BBCDGMVbozyqjd_ZRH=1& ~}}|vpida`beilnooponlieccehkmnnnptz}xwxz})Lo/aHld;v=In_SKGEEEEEDB@=:765566655431/,*)),036763.+((*/4:>ACCDFJMPQPNKHEDCBA@?><:8767899987668:=@CEEDA=978<ERd|Dz:{n-m2sVB5.,,-.00/,*'%$$$"  #(,08EZxG.{w&7U5  )18?J\u%a9LAyR7(!!$(-27995/(" #$$$%(+./-*&#""#"      !! 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V58\i:=S1n_S2L6qoYn/J]K?zj'9ABx5])~U]~XeW>QO.00z 16p ad+uiOM]V2DA iJUhe8/p{2<1xX'~wti|w_]SN`WSHD?>U4MI0C**HA"973@<(#!/.(C/&%$  %"  (""##( ('  (  !0( )!,#,'     '&  #""    ) & '$,(1%"#(  ,+$.+//3.)%,-3-280&.9Q*3$)''$'@(4@?7;6@@:75-2':8LS:.<>8:?8B<?K2JBD*LL7=PSa<FI;EGIS<B8BmNCROMQSIUXOMGFFK?dg_aOjBXzgZSeK+CTFFG;;WfV[*koqvN=OR(PssBjWT]YqcWGH[ZBeeb\\e\SR}fa>x{jcjPjhXa|fzlftx{{s~~q|kwx~~~~~TKa~~~~gvu==~RDbbb}b'~~sk\ppp,r\epZG80~pp3@zGpHwhMd"&LuzPdd|w|E<8;D2=Rpw:Yp|,M4;pp Bu@R]p&q,pBdpp0lp} Hpp&Hc,Hp2pjLppp;pRQO^_K6 :C1]*[$84C8+  ;[#*6cP32654+>,!4BQ(>@F!-+--E-+Y&5"5JW3;'' + ('2%>$+6"&+1?5,%#076@', &1 #6E##:!b"2'( *<% %$+#//(% 2(#9;(23!&"i"'-/!/#(',,#'+$(0>)?&F.! '! ;0M)1,2#&)2]!R8)3'92!"&=$87%\7-#&:T-;B%(+CA*,0Bj5@C2%3j+<f55M3]B7Z424)K:YPXe 5h_C8I,+zx=B+g":^KGcXUy\<C5TP<v9wm9XvYQ)]j@iR)mR]O;Pi^lgBOZm=RF,EhfpQ-KS]r17!rw>Qmv7GE~dQUe\]wC-%\Td^[c=6S'!GAAAAATAACGCTATACATAAACAATCACATATCAAAACTAAATTTGAATTATTACACCTAACCACAGAACACCAGCACACGTGCACTGAATCTCTCTAACCTGGTATAAAAGGAGCCGCCCGTCGTAGGGTGCCATTAGGTGCGCGGGTAGCTCTCAGATATCCTACAGGCAGAGAACGTGGAACTCCGATTCAACGGAGATAAATTACACGGAAGGTAGGCATGCCAGTAGCAGAGCATCCTCCACGGGGATGTATGGAATAGGCTACATACCATCTATATCGATATACCCCTCTGCAAGCGATCTCGATACGATAGCATCTCTTTCCGTATCAATAAGGAAGGCTGGGTCTATCGCCCTACACACAAAACTACGTACAAGAAGAGGATAAGACGGAGATAGCGCCGGTTGCTGCTCGTCACGTTTTTCTTTTAAGAGTTGTGCTAAAAACTACGGCGACGATATAAGCATATGGGTTGCATTCTACACAAGAATATTATGTCTCTAAGTGTTCTAAATCACATGCTGAGAGTATACCACACGTTAAAGACTCTGTGGGTTAAGACATAACCTTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 16:41:00 -04:00AEPt@%AEPt@%APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTS~gBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?VTDATA ?VDATA ?VS7DATA ?VҍDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM@hP1RL@dP1WD@mP2AM@vP2BA@@zP2RL@qPBAS@@T^PBAS@@RPCON@@aPCON@@_PDMFTkPDMFRPLOC@[PLOC@VPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAeJSPACQSPACAeJSVER4.0SVER ~\SVER,,/Scal@Scan@TUBEG6Tmpr<UserphARphCHRphDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt@%AEPt@%APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt2ASPt2B1Pt1B1Pt1BCTS~gBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?VTDATA ?VDATA ?VS7DATA ?VҍDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE$LAST~~|LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AM@hP1RL@dP1WD@mP2AM@vP2BA@@zP2RL@qPBAS@@T^PBAS@@RPCON@@aPCON@@_PDMFTkPDMFRPLOC@[PLOC@VPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAeJSPACQSPACAeJSVER4.0SVER ~\SVER,,/Scal@Scan@TUBEG6Tmpr<UserphARphCHRphDYbigphQLcphTRphTRvTLYr*X=+I}S$PS9Hb?j[]8|a)&p[>Se4#Y`,{[._sa$_aEC0'Uo,#$.:}jD(y$9eKobs>6I(C.F22jDTWy2>y^q3Th$Y thENWNv3[My co{l(l>6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^T[PXFWhw cUaczMJ^(wD14dNbzMd&8]t?E>+%3NWFB$BnJWXs9EQpEX D%"E(YkI#ys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;LL#_?6(CN@(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUG