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M~|zxvusqomjigeca_];OO GGGddd{{{   $$$,,,444<<<DCDKKKRRRYY[``egflllsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e53d48e4ff19432489129e94ac88de16Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_14-53_07343130Seq16796134879_134879mfagan96-Well%K1319_G12_Hoffmannia_conzattii_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z !#$$$%(+/220,&  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 xc@6AASjAHKB.bcpKB_3730_POP7_BDTv3.mobtermCGGCCTAACTGCTCTACCGTACTTTAGCAGATAAACCTAATTTAGGTTTAATAGTACATCCCAAGAAGGGACGACCATACTTGTTCAATTTATCTCTCTCAACTTGGATGCCATGAGGCGGGCCTTGGAAGGTTTTAATATAAGCAGTGGGGATTCGCAAATCTTCCAGACGTAGAGCACGCAGGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAGGGGTAAGCTACATAAGCAATATATTGATCTTCTTCTCCAACAACTGGCTCGATGTGGTAGCATCGCCCTTTGTAACGATCAAGGCTGGTAAGTCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCGGCCCCTGCTTCTTCGGGTGGAACTCCAGGTTGAGGAGTCACTCGGAATGCTGCCAAGATATCGGTATCTTTGGTTTCGTATTCAGGAGTATAATAAGTCAATTTGTACTCTTTAACACCAGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTTGGGTGACATGAAAACGGCCTAACTGCTCTACCGTACTTTAGCAGATAAACCTAATTTAGGTTTAATAGTACATCCCAAGAAGGGACGACCATACTTGTTCAATTTATCTCTCTCAACTTGGATGCCATGAGGCGGGCCTTGGAAGGTTTTAATATAAGCAGTGGGGATTCGCAAATCTTCCAGACGTAGAGCACGCAGGGCTTTGAACCCAAATACATTACCTACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAGGGGTAAGCTACATAAGCAATATATTGATCTTCTTCTCCAACAACTGGCTCGATGTGGTAGCATCGCCCTTTGTAACGATCAAGGCTGGTAAGTCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCGGCCCCTGCTTCTTCGGGTGGAACTCCAGGTTGAGGAGTCACTCGGAATGCTGCCAAGATATCGGTATCTTTGGTTTCGTATTCAGGAGTATAATAAGTCAATTTGTACTCTTTAACACCAGCTTTGAATCCAACACTTGCTTTAGTCTCTGTTTTGGGTGACATGAAAA%3<J[o 1HU_nz)4BQ[gs~ "0=ITamz!,6@LYfq| %1:HR]ht!.9DQ[gr %2?LUalz+7CM\gr~    ' 1 = J T ` l x    ( 5 B M Y d p {   , 9 E O [ h u  " . : G S _ j v    & 2 = I T a n z *6BNZgs&2?JWcp} #/;GU`lx +6BN[gs'4@KXbnz $0<HTamy #-:FS^jw -:GS^ju!-8DP\iv -:EQ]hs ,7DP]jw ".9FR\iv ".:FQ[eu %3<J[o 1HU_nz)4BQ[gs~ "0=ITamz!,6@LYfq| %1:HR]ht!.9DQ[gr %2?LUalz+7CM\gr~    ' 1 = J T ` l x    ( 5 B M Y d p {   , 9 E O [ h u  " . : G S _ j v    & 2 = I T a n z *6BNZgs&2?JWcp} #/;GU`lx +6BN[gs'4@KXbnz $0<HTamy #-:FS^jw -:GS^ju!-8DP\iv -:EQ]hs ,7DP]jw ".9FR\iv ".:FQ[eu      '4':'2:5303:"$:":)2621&61416::#'*::/&>>>*)>>>>>>>>>>/*>>>6>>>>>>>>>8>>>>6>*)0/>>>>6>1/>>6/>>>6>6>>63>>>>>>>>>>>>>>>>>>>)'>61>>>>>>>>>6>>13>>>/1>>>/>>>/>>>>>>/6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>/>>>>>>>>>>>>>>>>>>>>>..>6>>>>>>>>>8>>>6>>>>>>>>>0>>>>>>>>6>>>>>>>>>>/+>1>>>>>>>>>>66:::6::6::::4::::::::::6::::::::6:::6:16::::::::6:666::6:::::::6::::6*:5::3::0%:::-64::35:2:5:10::::6:35:633:051"**(2:5::5):32255$::6':,'63::3% 5' #236613-556663&16456(2#65%635,!%%%!6'33/1,5666'3%5566(53%4/& 5541444 5"3-5%.)/-43 55*(  #      '4':'2:5303:"$:":)2621&61416::#'*::/&>>>*)>>>>>>>>>>/*>>>6>>>>>>>>>8>>>>6>*)0/>>>>6>1/>>6/>>>6>6>>63>>>>>>>>>>>>>>>>>>>)'>61>>>>>>>>>6>>13>>>/1>>>/>>>/>>>>>>/6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>/>>>>>>>>>>>>>>>>>>>>>..>6>>>>>>>>>8>>>6>>>>>>>>>0>>>>>>>>6>>>>>>>>>>/+>1>>>>>>>>>>66:::6::6::::4::::::::::6::::::::6:::6:16::::::::6:666::6:::::::6::::6*:5::3::0%:::-64::35:2:5:10::::6:35:633:051"**(2:5::5):32255$::6':,'63::3% 5' #236613-556663&16456(2#65%635,!%%%!6'33/1,5666'3%5566(53%4/& 5541444 5"3-5%.)/-43 55*(  # UL:%6\-D1&!=(d8NC#?CK18HMOS7J)c=T;EDM^A)G\ 5MT2 4"c>'R7?O8MJG,^5O/$0(G=` _.7IR ``K ?)F9<.,[J\7 9:B-I%.$2 ,EV.ZDH!(>"?O\ D]IPM-J.$2F?dOZI?J)VSXVFN\(C1;CT7W1PH3 CLT@= "F48 /QX- -I;2`;"O:P029L,W7?J 30>^TOP.O*W:,=K).?:'0BX=\E)+&98H;Ic1R6L4M!aV"(%XI":_F*<1H+E=Y) < 632N8/ X?B*U Q *!cE  'Q& 57JC?7Y8O+cAQLV2aH(Y<!3 ^Z)B'1#5%(A9M GY)3@:6b=D4[W_F BO$GK<1#$A!YP% 3 /:2PTH;b,L^)$3D]<3 A?`E/+a06_2M_!LNV0/= D @.G" 2Y!2\P2_7 CO%B0D<Ee`/#tL/U)3u<~A>/viqbx}4Cxim^3l(y~1k#~9_zZfr";Y3R54!|B@vKtYi+EOuUT<Lv~<h  wrU#Vy5nN*g(n<j +h u&6E (eOXDEn_i| P5[fG ]gj=~;x:8w%'2fUK.F  6%%vivmM;t_O&BL  .|b<%:-DtNFr`sP`Fy0k/TEAA`qF2]wXaUGKaW*t2<W67uYQ5) roga ;yT E89 J{srb|_?dU}{mUXaJ_YNLE9Bm\NWRLKGGOE;5 547$2;5#S?'+'$% G0 (,(, )(% *'%-'! +1' ) '! !" %' ,&!#%.(/A3!)(!&#/$* "'. $#-!$,""%%'.!5')*(('!"+)'&!!!$)'0/',,((&#:,'..32. 4,+9%%9),35;2A?6K==6;%7@@?>0/45+72F:86:>XLO===?9PR@O@DGSFJGOFAE8XGYGJFS>O]BJVV[FBOKL?CDKH\HNPCVUhTPG\K`iKJRS\JGYRoKUOgMZ_wktZanghYlo_t}__h`rcslnum\pgbkmbcngqtmglktqtidmup}x{t|zky{rpK-z 1f mpSp( i@p~J?M:/p8,B7_R6J6, py#<y g9VK] d3K[0yB>j) e:p~M'Xp>NDCppp/pp'pp8ppp&bv/>' Ag g6F0J:XZ }gCQ%>4z&}i1 Ga9& H3(2 #M o72;" .5C0 "+>+!-$  ' %!J#3")+<-`'&C8 5-+%! #   A("*1& !"9#.-/C3& "6@8"1 $#"$#-),!#2 %(*?(0YN6'!N)*!$$*&51)l2=$:'=.)='$G *!*.P/3/2-/&&92XG1/1(3 &7d,EKFxM#?/8"F'7OQ*/b9R.&I!2>$44YK62B R'^e$`m:X!;2>)V,VP~6A5I vD"VbkxIy6EdPUH<!n3tGgsFf<c\"WdbW*Fe@x2[AWh('&L>PR[<sh@cZ|t&[~|9c<:,I$46 GAAAAATTAGTGCTATAGCAGGCAATCACATATCCATATTAAATTTGAATTATTGCACATAACGCGGAAACACCAACGGTCATGCACGAAATGTATATAAGGGACCATACGAGGTGCCGCCCGAGAAAGGATGAAAGGAGGGGCGGACTTAATACTCAGGGGAAATACAGGAAGAGAGGACAGACCTCCGAGCAAACGTACATAACTGAGACGGAAGGTGGGGATGCGAGAACCAGCGCATCATCGACGGGGATGTAGGAAATGGGCGACATAGGCACTATATAGATATCCTCCTCTACGACCGGCCTCGATACGAAGGCGTCTCGTTCAGTAGCGATCAGGACGGTTGGGACTACCGCCCTACACAGTCGACTTCAAACAACAAGAGGATAATACGGATACAGCGCCGTTTGCTCCTACGCATGTATTACTTGATGGTGAAGAGTCACTCAGTACGGCGACGATATAGCTATCTCCGTTGCCTTATACAGAAGAATATTATGTCACTAAGTACACTCAATCACAACCTCAGTGTATACCACACGTTCAAGACTCTGTGAAGTATGACATAAGCCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 16:41:43 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtzASPtzB1PtyB1PtyBCTS\BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ;`vTDATA ;`vWDATA ;`vBDATA ;`vDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~ZLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS/P1AM@FRP1RL@AP1WD@JP2AM@SP2BA@@XRP2RL@ORPBAS@@2PBAS@@/PCON@@?PCON@@=RPDMFTwPDMF/PLOC@8PLOC@4RPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%/SCAN@SMED SMLtSMPL&&TQSPACAmPSPAC/SPACAmPSVER4.0SVER \SVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCH/phDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtzASPtzB1PtyB1PtyBCTS\BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ;`vTDATA ;`vWDATA ;`vBDATA ;`vDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabjvFVocjeFWO_GATCFeat//j6GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANETLAST~~ZLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS/P1AM@FRP1RL@AP1WD@JP2AM@SP2BA@@XRP2RL@ORPBAS@@2PBAS@@/PCON@@?PCON@@=RPDMFTwPDMF/PLOC@8PLOC@4RPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%/SCAN@SMED SMLtSMPL&&TQSPACAmPSPAC/SPACAmPSVER4.0SVER \SVER,,/Scal@Scan@TUBEG12Tmpr<UserphARphCH/phDYbigphQLcphTRphTR-"3oAZKC[p3}$r&kUV'4Mu*}c+-O)C@5&J06THKoS]/~8R&}[>FESt%e]:aH<6.tJdQeK~jca1n6*X!afT7+|4C9|z*yEwiO*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/6?|$lQ%e+Bw9#MBz3)-I5d3g9kw6T-(WxdUo`($'tV&MWW|;WL.]_93Qq[%w%:T?NWFikas1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-=DaJ2`>nX$t!TQqvLWvq$~@1)(7tHt6Y^}9*6>JPqdC(w: 6IkVWX1\Mn-k|cy[*oCfo;LY&0E(_y"%_oU@tC]Q#V>AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnP_I[Dd@2g|5J_@A[]"p64y^M_9ni|\Z&}NV|]GHS\nX|L?g?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!3#C,F2id7-J!."^O[,z^E$6d+XnhcjPGh{;qeXd9U|q0Yl~F4R/QkEMh#YQ6;Hq.z/W@,CF 9HYp#W:P!gFS]T'_C:rF(5,f *"