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M~|zxvusqomjigeca_];OO GGGddd{{{   $$$,,,444<<<DCDKKKRRRYY[``egflllsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2434689c141a401bb4bb6f95ca84dee9Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_14-53_07343130Seq16796134879_134879mfagan96-WellK1319_E6_Salvia_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z -8<;86557751-)(((% 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"  #) 6'@0*4 1. 0 @,&#$).4%PG! )' ?$Y"#.+ ##$+#!<5!*#,!#u) (, '+G.&?2'%"H@ 8O+5F>TE5{#B#-D(P1Bi3T]#[Qu)BSt$ =7%GXE,&#!|w%kuA2_Wxx>[&@JY'0# zt 1S)DTDtw jrKV^NAn+Pt:xYIgReO_mD'~51)+# :wB4GAAAAATAAACGCTATAACATAAAACATAACATATCAGGACCCCATTTGGATTATTACACCTAACCACAGAACACCAGCACACCTGGACTAAATATAGCTAATCACGTATAAAACGTGCCGCCCGTCGTTTTGAGACCTTAGGTTCGCGAGCAGTACTCTTAGAACCTACCGACAGAGAGCACGGAGAGCCCTTCCTTCGGCCATAAATTCTACCATAGGAGGTCACCCCCTTGTGACCGCATCCTCCTCCGGGAAGGACCGAATGTGCGCCACCTTAGCTATATACTTATACCCCTCTACAAGCACCCGGTGGATGATGGCGCCTCGTTCCCTGGTAAGGGGGAAGGTTGGGAGTACCTCCGTACACACCGCTCTTCATGGAAGAGGAGGATAATTCGGAGACAACGACGGTTCTGCCTCCTCACGTTTTTCTTTTTGCTGAAGAGACAATAAGTACGCCGACGATATAACAGTATCCGTTGCCTTGTACACCAGTGTATTATGTGTCTAACTGCACTCCATCACATGCGCGGTGTCTACCACTCGCGCAATTCTCTCTGGATTATGACATAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 16:41:04 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt'ASPt'B1Pt&B1Pt&BCTS_BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 3gTDATA 3gDATA 3g"DATA 3gDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE(LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM@P1RL@P1WD@ P2AM@P2BA@@P2RL@PBAS@@VPBAS@@PCON@@PCON@@PDMFTkPDMFPLOC@PLOC@PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAd$SPACSPACAd$SVER4.0SVER TSVER,,/Scal@Scan@TUBEE6Tmpr<UserphARphCHphDYbigphQLcphTRphTR3This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt'ASPt'B1Pt&B1Pt&BCTS_BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 3gTDATA 3gDATA 3g"DATA 3gDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab+FVoc+FWO_GATCFeat//+zGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE(LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM@P1RL@P1WD@ P2AM@P2BA@@P2RL@PBAS@@VPBAS@@PCON@@PCON@@PDMFTkPDMFPLOC@PLOC@PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAd$SPACSPACAd$SVER4.0SVER TSVER,,/Scal@Scan@TUBEE6Tmpr<UserphARphCHphDYbigphQLcphTRphTRMr,c],~u:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;N }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V?7Z(oIqE9}YhnOA1Uh+!:EnRq&5h QbS9YH|O[nYnuPQh^5Q1V?yRl<`QV-@[3>>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["#87zFl?xSmh4il5A|M!3g-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW39nmG[l|$!}KZ 4w1X& u1p)!|3M[H#S_d.u?zdq3+fFc':Z^P?ZY{7+VDTj.uFgq%,NKo-,(B~bwj{}L]^k@_ajF=W.X}=";WjcHdkabCDkm[&HcXe"t}1%j-]Tsz^`A.u?s-u@G1fCy:*?GD8Y~Csk%pcyO_lh#m|!yrA]#7w"OMbq?Eb,Z#GlOjW^]=7l1%Em[.YYo%/D^*Pis|K/l3672'[)VBT9\qwH=qd)B*E\jE@r9#$%rnLjL3ro{`N%7`h?D,kSagv`k=U@xkk^CELhhSc%bVm? >S]*|QK:}$OEH3<("Hf.Q+s3TtGK><3$G,lwd-l#frL?*Y^gM*yAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt'ASPt'AUDT9B1Pt&B1Pt&BCTS_BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 3gTDATA 3gDATA 3g"DATA 3gDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab+FVoc+FWO_GATCFeat//+zGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE(LAST~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISP1AM@P1RL@P1WD@ P2AM@P2BA@@P2RL@PBAS@@VPBAS@@PCON@@PCON@@PDMFTkPDMFPLOC@PLOC@PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%SCAN@SMED SMLtSMPLTQSPACAd$SPACSPACAd$SVER4.0SVER TSVER,,/Scal@Scan@TUBEE6Tmpr<UserphARphCHphDYbigphQLcphTRphTR