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***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR M~|zxvusqomjigeca_];OO GGGddd{{{   $$$,,,444<<<DCDKKKRRRYY[``egflllsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5732c4822e6546f0820415ac9c4f0e08Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_14-53_07343130Seq16796134879_134879mfagan96-Well!K1319_D3_Hyptis_atrorubens_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z 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" ,.6:66::41:6':::):4'-$:*.%65.2134:41361:::) :6.'11%::+:-65'$:)(:'/:52*3:1'2(13 :*15'2(:4))/::34))4.:)4'634*4655!14''5* & HHY X62 FCA=c%cI0#J2->T!-X"J!*<V>WD$RE:$(QTO1(@Q.1B6"JFAH ,BZ*5I.RUB.N -$V)SW>]cF=GI9D=%92%<2 PC 7Q5F<;H`A\'X< V.3 Z3`6"ZB%S -RW]841S?+a(B0D6!S@72DC;WE; <H]PJ39;3$Aa[%R\9 ?+ 7YT$'Z+U 6E3^0Y*)FbX(U"/;V=*(T"Q^,dBQ9<G:P(>=P<=<K>6N@_IWL*8BXJJ 0,DQ4SO!" 3J=&GCV6T/J>'`)ULF\6FTO 47 A19GB43;.%CIC3I)[TC :$3]XCc=66> 2\LJEV3 :(*UI!=0GcN/KC;FX18=Ac'`&*+& 6@> I **4Q8R#K3  D .W&JAL=XE_UL;"3=I&> RTE?ABV$c8" a(,;C>X/  F &>8 GYJ29G,^>$"0*=@>2& '2=3<&&>}a2u0c(2&a&)7Px$cG ^C'E) 8v RDkh`"wJ?UW)SZi vv;E,(;C/Lvh7AoafLA5CA%Xmma }M!nk $S;EB~];wO6:(rB\lVwlzIw4o{JYytS)FGrs ^S'5;pP _Q[b<3cG/a_xCnuoN"20nv$.gu/FE6K1gGL5\6'9%Z mbXm%<W)hDv!q  :kS;S7%z|K TuVu,!SaT/C]g X>PsGq` ddO4+&npmtirkqmX]\GSX_GK??OKHZZJP>AC<;8LF3&0<:3 =9#") ! !%%$!))*" &4    "#"  .#% (                    !  /! "*"!.)#-5 $ ,!".),2:"85./.( "/-)$0"1!B.#%+-&B+&(5),5+&"$#")*0/1*>0N2.:M1;+37"09.048*'*DG5#5,3@K14:9360.@+A5?V.H;4.1/RE.<AA30MK:INGKI~~;NC9;VO^HKECLRDTVHQFLeXeTzlQKIPjjP@`nSk:WB~MRRgnZfPElUojRRaTKifc\pRRQPtSq 3;t}vY^jjj0SSSSRRjRRp<=p.X Qp>wd}C5./)?DAy[piV,ud8i8:NY[.*7y\!<8/nzXV  ,8p Dztp9PNpBpsq87l&8Lh880pm8 .8&xpppjp@p~p p~GB 2sunyC{~@[Zo7=//%CVXQ ?OA&'\VK* R/**R5]9k1?9H( )!  ."!u]**3:9 $ '1 $1"#%00&" "?+  1*' )* +"%)$&7 " H$51))$$ $' !N#" $-'%HC3'&*8&$!"&(4G" -*&)1&%.!%;-&#'%!(#02V#).8.5& CH/*,(9">H),Y1(,,-;8[ 6 "%";;0;%Cf49};5"}X@$VuWCm,N,4$km},=j[9F'gkP3.]#`Ypl<SI6gu ]-hA:m$RQyXt*he"HX2Z*ogU)lH<^}zw3,Bx!L<qGAAAAAAATAACGCTATAACATAACCCATCACATATCAGGACGAAATTTGAATTATCACACCAAACCAGAGATCACCAGCACACGTGCACTAAATCTCGGTAATATAAAATAAGGCGAGCCGTCCGTAGTTGTGTGGCAGGTTGTGCGGGGGAAGTACTCGGCGAGCCTACAGAAAGAGAGCACGGACCTGAGAGTAAACGGCCATCAATTCCACTGAAGGAACTGATGACAGTAGCAGCCCATCCTCCACGGGGATGGATGGAATGGGCGCCATAGTAGCTATATAGTTATACCCCTCTACCAGCACCCGCTATAAAAAGGCACCTCTTTCCGTGGCAATAAGGACGGTTGGGACTTCCGCCCTACACGCTCGTGTTCATACAACAAGAGGATACTACGGAGATATCGCCGTTTCCGACTCCTCACGTTTTTCTTTTTGGTGAAGTGACACTAAGTACGCCGACGATATAAGTGTATCCGTTGCCTTGTACAGAAGAGTATTATTACACTAGGTGCACTCCATCACATGCGCAAAGTATACCACACGCGCAATTCTCTCTGGATTATGACATAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 16:40:52 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt8ASPt8B1Pt7B1Pt7BCTS$BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 4xhTDATA 4xhDATA 4xh&sDATA 4xhcDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~#aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS[P1AMAP1RLA P1WDAP2AMAP2BAAA! P2RLAPBASAAPBASAAPCONAAUPCONAAPDMFTsPDMFrPLOCAPLOCAPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%SSCAN@SMED SMLtSMPL""TQSPACAdSPACkSPACAdSVER4.0SVER $SVER,,/Scal@Scan@TUBED3Tmpr<UserphARphCHphDYbigphQLcphTRphTR5This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt8ASPt8B1Pt7B1Pt7BCTS$BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 4xhTDATA 4xhDATA 4xh&sDATA 4xhcDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab3EFVoc34FWO_GATCFeat//3GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~#aLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS[P1AMAP1RLA P1WDAP2AMAP2BAAA! P2RLAPBASAAPBASAAPCONAAUPCONAAPDMFTsPDMFrPLOCAPLOCAPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%SSCAN@SMED SMLtSMPL""TQSPACAdSPACkSPACAdSVER4.0SVER $SVER,,/Scal@Scan@TUBED3Tmpr<UserphARphCHphDYbigphQLcphTRphTR-'Icf]h_m7^1V .Uw.PmaSj4q+E[7VGJlqbQ9dHUslGW[#xjT|T6q32&L"p;I`NjYid{0PajeoI.*R@_aA+|1hygSHmT;P6[s$\o}Z'BNpW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`hp}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9)]ZlB2K4]J7UZGfC@LW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A|!Y<,h``z&^7a."sWi`A(^:go8m+adJs _ZZ^S_ngZ-3$tir"8|oTq}A|1D@<7>{AXG9{$5t{cn&|Bp\2iB];W phI,9^Q>4y7SEakZZA/Pm]ECGKXW .C5wz^`ZdD\4ApZTFS$oa$Fl@i2go~WR&^.|bEdG;_{8@hh/WZ#3|5O-U[dUDNxGq@p'Ic62v^&Gd\{diO}L6>U{mwYBTW .Nio8-I1]f\+P4avFwn&K2f_bA[]/|;vn5mr+8/Yb~;H7.N{CG2CQ>b{`$dbG_?lE8$kabCMV0|>kij7!}WQ`Q&]t[XS:.\_'[wA#Q9Tc T-*<^m9,NzVDSBrRL8~f)NKU|t2#\7%LGARz3BZMJh:t+V8!0.9,(B~bjOe:=KKR"\3|-bWF'zP}6Ja!8UY59D4LHmXY#agsC!.:QI5&.XRz+*}Lq&Wi1C9])I*axEEw~nmm&cj60Z9Fi/cE`\P \hWW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUG