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M~|zxvusqomjigeca_];OO GGGddd{{{   $$$,,,444<<<DCDKKKRRRYY[``egflllsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5e3667c1b49b4c9ea428d61cd8dab64eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_14-53_07343130Seq16796134879_134879mfagan96-Well%K1319_D11_Solanum_myriacanthum_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zxrnlkhd^XTQQRRQMHB=:987678<AEFEA;769=BEFEBA@ACEFFFDCCCEHMRVYYXWWZ`gnsx~&?[|,BYn~vbJ0}S- oXG=863/)$ !"!"$%%%'*/379;<?EP_t^` Sy>A3kS@3,*.8FWk Irb7 v\G7*!     *;SsFQRx*rL2# .B^$l?j}wX'bmE)"+25630,)&$!         *Gs^-N~+j]GC      %H| j| ['        "%'(('$"!%*./-(""?vFpiSYv"u|e%r/U0! k~ToJt8").257984-! ,f:bNzgiz4 !&*-..,($      ""   Foekd0    ""        "!   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H2A@RId&0=2K1UZfSX'u4i76t%?UNjYTO>`axB{J*sR~Nr?2!;xnVe<xb{%iDgE1VIvb,F^-elu;= *GAATATTAAATCTATAGCAGAGAAATCACATATCATTACTAAATTTGGATTATTACACCTGACGACCAAACACCAACGGTCGTGGACGAACTGTGTCAAACGGACCATGGGGGGAGACGCCCAGCGTTGGGAGGAAGGAGGGGAGGAAGAAATACTTGGCGAACCTACAGGAGGAGATCGCGGATCGGCGAGGAATCGGAGATCCTTTCGACGGAAGGAAGGGAGGGCGCAAGCAACGCATCCTAAAGGGGGAAGGAGGAAATGGGAGCCTCAGGAACGAGACAGTGGTACCCCTATACGGGGTGGATGTAGGCTAACCCTCAGCTTTCAGTAGGAAGAAGGACAGATAGGACTACCGCCCTATACAGAGGACTTCGAACAACAAGAGGATAATTCGGCTACAGCGCCGTTTCCTACTCCTGATGTTTTTCTTTTTGGAGAAGAGACACGAAGGACGGCGACTATATAAGAATCTTCGTTGGGTTGCACAGAAGAATATTATGACTCTAGGTGCACTCAATCACAACCTCGATATATACCACACGCTCAATACTCTGTGAATTAATGAGATAAACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 16:41:45 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPttASPttB1PtsB1PtsBCTSLBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9trTDATA 9trDATA 9tr:gDATA 9trODCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~KELIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS ?P1AMA6P1RLA2zP1WDA;~P2AMADP2BAAAIP2RLA@PBASAA"PBASAA rPCONAA09PCONAA-PDMFTwPDMF VPLOCA)vPLOCA$PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N% 7SCAN@SMED SMLtSMPL&&TQSPACAk=SPAC OSPACAk=SVER4.0SVER LSVER,,/Scal@Scan@TUBED11Tmpr<UserphARphCH mphDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPttASPttB1PtsB1PtsBCTSLBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9trTDATA 9trDATA 9tr:gDATA 9trODCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab[)FVoc[FWO_GATCFeat//ZGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~KELIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS ?P1AMA6P1RLA2zP1WDA;~P2AMADP2BAAAIP2RLA@PBASAA"PBASAA rPCONAA09PCONAA-PDMFTwPDMF VPLOCA)vPLOCA$PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N% 7SCAN@SMED SMLtSMPL&&TQSPACAk=SPAC OSPACAk=SVER4.0SVER LSVER,,/Scal@Scan@TUBED11Tmpr<UserphARphCH mphDYbigphQLcphTRphTR~WhC0-#JpPJwiO*$]_`#?Z9rS}G|u8 ]qU(6ji,x5?h8]Z~m$F5:U~2C 4"])|JjJDeV2E+qmj4I73wJzO`P{ e,&o.]/+M;`8hrjuO*@NzRoB+ks9?OkZKUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^M4'Uw\Pcc{\A_}$A>AR5Z(b|U2mC!KC_KdrWS[jWcgaWmUbe\W'KFkaqQltX[3>>B~:^gWiD!}%`8p3cma1yM\9MBEqJ*(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i6ti#O7(V6[/3-6)SE8pT/@MOsijd(DUfb0be|COv(0?.DCBzID(#}H4A|?{X D%"E(YJ{kys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;LL#_sv([7@(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4:4L\\W'KFxz2jA&owbBV*d>AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPttASPttAUDTi<B1PtsB1PtsBCTSLBufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA 9trTDATA 9trDATA 9tr:gDATA 9trODCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTab[)FVoc[FWO_GATCFeat//ZGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANEYLAST~~KELIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS ?P1AMA6P1RLA2zP1WDA;~P2AMADP2BAAAIP2RLA@PBASAA"PBASAA rPCONAA09PCONAA-PDMFTwPDMF VPLOCA)vPLOCA$PSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N% 7SCAN@SMED SMLtSMPL&&TQSPACAk=SPAC OSPACAk=SVER4.0SVER LSVER,,/Scal@Scan@TUBED11Tmpr<UserphARphCH mphDYbigphQLcphTRphTR