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'        n,,,III^^g{{&&&999DDDXXXaaavvv      ***>3>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR M~|zxvusqomjigeca_];OO GGGddd{{{   $$$,,,444<<<DCDKKKRRRYY[``egflllsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8a6534cc11e34af4b9745855d6e083daSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_14-53_07343130Seq16796134879_134879mfagan96-WellK1319_C2_Aldama_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z'1;ACDFKS[adca`behhcXK=0'xlaYTTUVUQJC>;<?CGIJHECABEIMPPNKIJNU\`a^YWY`ju}|{2UwHx/WzvV.]"wEzdRE=99;<==;9520/148<?@>;730///0/,($"%&&$"! !#'+05:?CFHJOWe{*nwC@mvT:)r#wkeceiq~Jv8JSP@']-dM</'#! !!! !#$$$"   .Ef \Tv+}(oN;5=T|ls &+RiXeH5,(*.26888777774/)"!#$$#         #<fZ,NeA T,{~7  !       +ZfHbf3        2rWWlAck@EiBQC-re0rs2M0qgP)$+0342.)%!!0Ri4< N" %-22.'         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K3@&@/</X_'#F+`I<C P+'$#`X V4X8*TYO%SN89#268W 0RMT&Y;2"T=E0LZCI+>:''AZ,P6 4 1c 3M!*O "L#33X_:9K 9!W&U=ZL*/PHB\ Z[:-!D@<EHT;Y 0bQab%5 >2))\7:L/(C8`N#+ V%^( *?%=B4C&=C`FR01I:ZEU352d, 2?D*V([K /5Kb_'APFRU]E" "D@-[ZT_JK7R--'CaKQ[8(B#EIA8,8LE,T0-K PEMa9S,]))5 (A46&/E(> =#;BO?041(E@IV\ JK=9>(/ZW,=aWI7Q7YX9UQFWA`"9N8Bb/W$2BWD)V.,8CKJIH E.WAQJc! 57C0 0V8 MPWE:]J E!]N ZMQ>2@ZW/$C:#0GZ 7 =L_"1N 0LE)XM@H=:/5aO4WY^-R ++V?/<FDO"E2+:)*ZR=2'822*L?:!|r!\n~]L2*Yk,j]\.&P Nb}DTv3c1 %0Bf%ix$xa,"|P2>e]%haN7 xwN[ysyVh.[ 9mW0bB]joJDz Z6Wz`1l CN>*-J`YMKV/:o3#a~&XCM.&PE* u(0<{Q89haY)?#Bma_8lVD/xYi#jp9gcssi` u8bV-LhYblX* vezV^-.Q6LsLJtb<cCz"}vW;l;Q{( iXQ q$f6|rUB9s :0-:vRC 24V$UvHX_z^m";q+1 v`WiUe;ytuttUYVEPGPB;=NPR@P6HBJNS?;;7.%),)'#7<9!##)$  #!)93/8! ) %:, ! 4'%'+":,!        !        !$0$')+&%'&6#!.%-"8.%!&$"'$6'&D)1 :.")!3+39*/I'7?0*,64096.-%24@612?8@>:A"65=3;)=SDH9@9(@HJ8GBK:G?/$E9@>9BWC;S^fKOQL@BLVM=XX1H/G-8SXd;X-8MX\{XXQ^UFRk[O>IHXLfW[bdGU^KOlTeaKT`_t]T_fa~~TI~Islj}ahxrtxxlixzzw{v{{{xxw{~~v~~~KiiiixIx|xxxxxrdq6WPpLp8~?'`m8nT[|p ,0{\n<p!+|V;SI'2XQp``+vkylMiU^\>.D:opKef|bFL kxdwdp8dpa88nzLppp,*pp|n^NML&&'JGc "@8j'Y!qqOU))4gd/ 3q v"=T/( (c#  b Z94 ~/&x&&!-/#*".)?)S '  #*1+-0&M/(9+ "+$8-& ,0(&'I,&%%%A93))+"+(&"7",)(%!")6>19+ ,.*""E3!%! \&5()+ 9 % #-;'.34b'%,! !7*81(;-"YO6,%!AO(H2F# (S 77 C F/!#7J<"c D."[AhM3I@5K1?wh@&G]E{bk]:rNO"5<M6>CB4@Y QfsE<H=O6+BBq?A!?0cJSEP%k}=`rplf|&Th]TYd^8N`\Z-8_^k pblut"*?`P5CAAAAAATTAAGCTATAGCAGAGGAATCGAATAGCAAAACCACATTTGGATTATTGCACATAACCACCACACACCAACCCTCCTGGACCAAATATGTCCCACCAACCATACAGCCAGCCGCCCAAGGTTGGGAAAACTTTTCCAAGGTCGCAATACTCATCCAACCTACCCAAAGAGAAGACGGCCCTGCGTTTTTTCCCCCTTCCTTTCCACCCAAGGTGGGCACGCGAGTGGGTCCCCATCCTCCATTCGGAAGGGTTGACAGGGTGCCATACTAACGAGATAGATAGTGTCCTCTACCAGCAGACGGACGAGTTCCGAACATACGTCCCTATGAAGAGCGAAGGATGGGACTATCGCCCTACACAGAGCTGTTCGCATAACAAGAGGATAATACGGAGACAGCGCCGATTGCGAATCATCACGTTTTTATTTGTGGCGAAGAGACACTAAGTACGCCGACGATATAACAATATTCGCTGGCACACACTCCCGAATATTATGAGACTAAATATTATCCATCACATCCGCGGAGTATACCACAAGCTCAATTCTCTGTGGGTTTTGAGATAAGCGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 16:40:43 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt8ASPt8B1Pt7B1Pt7BCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?\TDATA ?\DATA ?\SCDATA ?\ҟDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FWO_GATCGTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~} LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMAhP1RLAd>P1WDAmBP2AMAvFP2BAAAzP2RLAqPBASAATwPBASAAR6PCONAAaPCONAA_PDMFTkPDMFRPLOCA[:PLOCAVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAfSPACRSPACAfSVER4.0SVER ~SVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCHR1phDYbigphQLcphTRphTR#4This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt8ASPt8B1Pt7B1Pt7BCTS~BufT JCMNT 0tCTIDJdCTNMJjCTOwJxCTTL 0kCpEPDATA@DATA@DATA@DATA@DATA DATA WDATA !DATA %DATA ?\TDATA ?\DATA ?\SCDATA ?\ҟDCHTDSamDySN 0LDye#DyeN0WDyeN0\DyeN0aDyeN0fDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0 FTabFVocFWO_GATCFeat//GTyp!!0+HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~} LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMAhP1RLAd>P1WDAmBP2AMAvFP2BAAAzP2RLAqPBASAATwPBASAAR6PCONAAaPCONAA_PDMFTkPDMFRPLOCA[:PLOCAVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAfSPACRSPACAfSVER4.0SVER ~SVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCHR1phDYbigphQLcphTRphTRaB$T9F_z"\=QtAN-GQ6)lI PK&'Kxf]$bN8O2Rz7}062sXaA=&@X<1p3QLT1;g8kY+-^m]ZOQ>\G`HK_^_<~P[Z66YM'KvT+F4$U]]ReL#(79pA$den?R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb_v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r3oJ6l&O`x Pz><3&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hygS4-X;i+-'+1T:o5Q*oc0c3\EI`N^vp5cM_1h\j[<63*7wJ7{_O6?@dsgbk1^>7(FTc%{M*AqbEdG9}j~:HzpWI23Z;|T92JNM4hzCreeM'*b*j_8/FJrlM;8'i2{js.>`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6LPyr{;qe&Ker"&O93s'!byH:/U?vw3(Qgc{?Eas1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52r =&e=.7I 9'kT'_C:j|(5,grOT1 x>|3d|BH:nklNK+T5L(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@C:RA;iiZhn|Z2#~p>wMXG9{k"K-Dty{J$XXt;J0Fk(l=[(\UlK>s\3zH'IDf+p&,JcAQ9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]wP1WDAmBP2AMAvFP2BAAAzP2RLAqPBASAATwPBASAAR6PCONAAaPCONAA_PDMFTkPDMFRPLOCA[:PLOCAVPSZE`PTYPJPXLBRGNmJ\RMXV1RMdNB8RMdV1RMdXBHRPrNJ*RPrV1RUND RUND RUND RUND RUNT 59RUNT (RUNT RUNT (Rate 0RunN""J:S/N%QSCAN@SMED SMLtSMPLTQSPACAfSPACRSPACAfSVER4.0SVER ~SVER,,/Scal@Scan@TUBEC2Tmpr<UserphARphCHR1phDYbigphQLcphTRphTR