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MJiyuw`QJ?+ <EXe(>HcG.|iZI5) ",,III^^^{{{&&&999DDDXXaaavvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRr~}{zxwurqomkigecb_]uOOOGGGddd{{   $$$,,,444<<ADCJKKQRRYYY___fffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7b02c2b975d1443b86bfa949056ac9a7Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_13-06_07333130Seq16795134877_134877mfagan96-WellK1319_H7_Persicaria_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z )Fk +Hcy)<INNIC<7569=@A@;61-,---,*('(-6@LV\[SE2c7 iR=*+HiS4f/=FHB0lI+x_H5"dG-{lc[UOHA;866787530.-.-,(# "%*05;BLZnKM 4Qbh`J%l#\'rhdcdfhkr~1rhXO;ZotgGHvt~ G.x9}O\C4,)(''&$""#%'('$ &-39BOb{%7Jb gF}4m9g`j08<NR1 /BWmhP:' !(/8DVnrQ5,YS$C*jC-t?+9GT\a`ZO@/  &8Md{qZC/    3OqAoX[E'd.  #$!  !7RqdF+  "G ~?>sl7  %*/48984-&  "8SqfO@;DYxc@%  %),-,("%4Ll<?$$f`E.  !$&('$  &9PkvZ@+<r+ ba 'i/    &?Zu{fQ@4./6CUhxs^E. -v;xs9uuAP#    1yccjq? &8Ng~~eJ1 !-<KZhqrk]K?DbPd])  >,+Z?P 8 -If~lP4  (9GLH;)    $1>GIE;-   PK[7vj@Q"YriBy@$Z.K5U2DQYXQC1 3HX^ZM9% #5I\imfWD/  &NIjlP]2 S7N0z V$  '7HWad`\ay\IP)w b. 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J67MS] VR--R4`F*!\]LC /N$=Vc=<F,>$2WE>PE2W9^[Q-1c)?-]L@E+)^X 43`EM[ !/G2!&?TT3% V$/VG `\+N  D2/'R++ b G+V9G5EW "B[Ga)%PI8bd<FU7O#-?OG(U`7H,+0\W2X$4]"6? )W2 !, JMXB-W-'> JW6V^,X,..,B1c %H-I0YU;E),@EXFZ a%< 0<(xxp s%.: ,OQ6L[c<_d_0; e7vtL; +zU%rn.] 2olaJHUX0(\)%+t&iAtX.-Pj9a^||T'SWD\V[D   4r[o >mTK<~!:b;/^/b`5^ ]E5UG1M~C t2D){Ijc#J bvD*?Dna\:R5jRX{>W4es.8)8H$?S{q-oM Zju(^=LNXIs1aR9%M'^dd,(J + 0] 4Z!3OF&`c!Eb36RR)CLi-y{'LnA~S<  P B'\sXjR}<[r'z^^gk]^MbqWGVA-dtDD;:;6}IeNRX<-3:ZE$8&'K21., $''+('*-#  #$$$*81 &?6=$/Z11) !5 ((-$""   !.! + ' +;1%,>9$ <";%-.6 *2  +  %#'$ ,0>%21 6+# ,"$--(6#63.?(2+=1'G9.$00742B8/" *Rb'IE3'g#48#8E/?B273VJ#+/H7?%cGN)IISDI_KfRYPIIIZSVVSS.e_DPeIIIIIIwI6xfficc<_Wgo]~[hfffpwfffgg~cQp\o\a\jizpX[pG^WvrkrrrrmufqT`T[~Pn|PtnrFjtrs{kkkk+,krrrr "aohxpN|hepi}=p*>&(@aWNXp+7s|%SC$5Q= ,zRC(kpX~kSFWoAHIx{PEHsC8p(pW3+9M:58NdHJ|Wn p#,U!p i`pp"80a2$V8X-Jh,8HSn6ppPy,P!3FXp,p,$pTpp&pEpp_wR j2FxZZ(E{"9MD<+WQ;{q^+qg+({uV \ >. _LY80qQ/sj~"(nd <%roH"}bM"I= 9%#D"k|o"3)vn|Oyt5!  :&:.C+wG)UE]*:CB&-, ";#$9*91W). 6+1._D32/!@3()?1"1:N,5'!#0"(#3IW0'<@41'?D'93#6BSJi+5-A;#[RF/)L7a1?-?6_-G? ]g>- h]NiFGbkqwRo3;*DKCHCJdE}O6?QZ-OP@x/QJR YTWim@bNbSZ5GN)=f^$IDe3:DT@22f\[D!-6MiUUhL`S2~PoE9M`KR\vy)gVN$EDqU)ygDD!J">m+d0GAAATTGGTGTCTCCCTGTCGCAACGACTGTATTGCCCTTACTACGGCACTTGTCACGATCCAGGATGCTTACGCCATCGGGCCACATTGGCGTCCATGTAAAAGTTGAAAATTCGGACGGTACCACGAGCCCTCGTTTTTCTCGGCGAACTCTAGGGTGAATAGTTACTCGGTAGACTGCCCAGATATCTTGCGAGTAGGGCTCATAGACAGGGATGTATAAACTTAATTCATCCTTGTAAAACCAGCCTTTGCTAACGACACAGCGCATAAACTGCGTTTGTGGTACAAAAAGAAGGAGGATGGGGCAACAATCTTACCACCACCTATGGAAGCAATTATGGAGGTAAGGACCGCTGTCCTCCAGCTATGAGAGAAGAATAAACAAGTATTTCTTGCGAATAGAAGGCCAGGGTACGCTTTTTTTCCAACGTGTATGGGTGGTGAGAGACATCTAATCCACTTATTCCCGTCGTTTATAGCCCTACCCACTAATTTATAGGTAGAATTAAAATTCGATGAGCAACGCTGTAGAAGAGAATAAGTATGTCCTCTCAGTACAAAAGAAAACACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 14:54:00 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtVASPtVB1PtUB1PtUBCTS`bBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;wTDATA ;w̍DATA ;wDYDATA ;w%DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~^LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS3P1AM?~JP1RL?~F$P1WD?~O P2AM?~XP2BA??\P2RL?~SPBAS??6kPBAS??4,PCON??CPCON??APDMFTPDMF4PLOC?~=(PLOC?~8PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%3SCAN@SMED SMLtSMPLTSPACAi3SPAC4 SPACAi3SVER4.0SVER `WSVER,,/Scal@Scan@TUBEH7Tmpr<UserphARphCH4'phDYbigphQLcphTRphTR9This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtVASPtVB1PtUB1PtUBCTS`bBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;wTDATA ;w̍DATA ;wDYDATA ;w%DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabnFVocnFWO_GATCFeat//n}GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~^LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS3P1AM?~JP1RL?~F$P1WD?~O P2AM?~XP2BA??\P2RL?~SPBAS??6kPBAS??4,PCON??CPCON??APDMFTPDMF4PLOC?~=(PLOC?~8PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%3SCAN@SMED SMLtSMPLTSPACAi3SPAC4 SPACAi3SVER4.0SVER `WSVER,,/Scal@Scan@TUBEH7Tmpr<UserphARphCH4'phDYbigphQLcphTRphTRf\mci2a>qN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`AqhjYS`N#db{we2Ns],.|] 2,\auB9DPK#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OLogXY@ZB 3O{ ~VJ(/on}sF{AR8=(V-OGC#!^MrrG]fQ.sBU4/Ce=N}tRw3KF;LsqBlC#hlTI#daC.?bFW&a:OcPX*F8BjhsDgn_RJ4ir$GE1Xz.?e Ml1}ssb[l"G>=?]p&k6v)ukO"yA&d}8qQgE5]U'j*-[~!Tq*nEg8Z~KI'_26?^V$Yeaejp~{>ZHm? >#veu]@|Z5hf6]X?K90#qN8>o XS_xP=O_l;mqL}4|Mdna'"`k=H<6.tJdQeK~jca1n6*17Uo AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtVASPtVAUDT|B1PtUB1PtUBCTS`bBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;wTDATA ;w̍DATA ;wDYDATA ;w%DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabnFVocnFWO_GATCFeat//n}GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE1LAST~~^LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS3P1AM?~JP1RL?~F$P1WD?~O P2AM?~XP2BA??\P2RL?~SPBAS??6kPBAS??4,PCON??CPCON??APDMFTPDMF4PLOC?~=(PLOC?~8PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%3SCAN@SMED SMLtSMPLTSPACAi3SPAC4 SPACAi3SVER4.0SVER `WSVER,,/Scal@Scan@TUBEH7Tmpr<UserphARphCH4'phDYbigphQLcphTRphTR