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  $$$,,,444<<ADCJKKQRRYYY___fffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 41f578f5015e46a98bd4fb3c8454978fSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_13-06_07333130Seq16795134877_134877mfagan96-Well$K1319_H10_Solanum_lanceolatum_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zEKVj9Vr   !%*/21/+)(''%!%/9>@>:521.) vU6nS=-   .Fb/`Ak 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"(1:63::::::63:3-::1.:::355::313::2:53::):1::5::1:1('3:3'1::3:0::235:::',,226:3.+::6)),)3:26245526:63:+2/2:"*442623&,22 ,#"QG ZI0(AZJ \3K!P ^9?H0%[U&13&S/9 O)" R:Y_ TUM $61@80 3&R-J["KH?;A," /7N'U/RG/MXIWAP%=TA&)^,'A$#3$^+93L_L:')8O.LJL: Y K9VX:C!\:>_9 D] 4.A9!b& 2!F<M@V T(TZ2GC :+2$\UU3LM]Z(+(IO04R)A)\",Ic$OU&6Hc92WaS%.T#"bJ3@7P A3&P 6cc5 O[+JMQHD?+. 2HORFEG:>.HD^\d c+:[Q[SO8ZTYF7?5DH9`"7 1IF?E55U 3JT21AJb6Y`&6'\AFD_I%A8DR 7Q+PT'b- R&.JML4.BE*[FK8) 8G,#TM"N/JO9_ 0@7SP!C+02JJ&97IQJ(7, NP3D Bc &D =BNQN]+NKO 3( Q6F 7' E 80+BI=H=U@]4;I$@6@O2'b7 N  E02=A@690`$A1\-?+ R2# VH_)5 p,8B{)1X]WiDU2 g_b+]RwNDFV5Hy9eO5!p IbtYU$$5Ev)pR1)"X2U 'YHZb+q+}pY!g%?`qYjx{:wr!}n1u&U'*0i'g~ np_8Ls?I%yo_FFu=L2GA*5/k<.J]rGAk(;QzIx]h*":\,AoH;b ohZN!h3@ _cWX'[f)-C5DSKvVgA.q{>Uas/Ys#:[u)PF~a+3 ^7/g p A8. HZG D~K r#sz{zlbtaor\eU\BrITssmXlXB?WE @RZCL@>87<1''/7A2).#$"'-.'/351'$#"")%# -0((!*! -).)+&<*'" # #    !$        -!#*-.)&/9'+(- %&   #    "0  8 ,5%,&!($*3($+-'J8#0))")1/3,,#'#2.)*6.-9=,(./)19#93K->9671-\LYF^JUA3CMDJ7H.FLE9JCD;I/G9FBRN@;N@=M9?849JHPPPPDIIIPWQgVQWUbCTYIcIILHM?XvvVVQ[~[pYnQZOVd`MccA|_]]]DRHiEQre~Sz`SbpyyyUVW\^oq~dqddiitpvZDsCuox4y|yvM}Jyyyyyuttttu.=Bs,mQptppV| uUBpI!p~dR1cPs8%,.d%,qpG,!.dpT8$Sf "bYEr/Jse dd)8<LL%1y8,VhQT]KoOTwVJ D}Skcd%He?fOwTp4pdQpAptO  Y %Mdh[+CQ.zvF>TO(icSN!+!2xvu,,Cfe(1)5a./5*(+$9)%&L52(6._) &+*#0@g@5G.O#.@'#))'#*&6$4+080. !/O%X1#(1((JFF57:)+A92&(0!$)5%<7!O)>?$8,);*/'%O'2A; $,65'5)//BRT)]IE2+!1*%%.-, !)4>W.*<*;0#"42R qj <T^ROd@1%.M4.' 31.)1<,F+2#G. 23.(C?AA1-F<%<49 /+5"2"$($..'/&-1&4=<B/I.l79E42Z#*9(51Cls4'?3I.8>*'2.9HJ.@,Y2O"&m(0S)-JX%38Uug1IG[/z[,ND9,04?08TYL%ZG,"m[W$$9W-<P=kXLNXvS0L*xVeqrWvIO1B3?G@)"}(*|O;3z{ZBPmJse8AYdPE|InK6yHOvS]Klf1& GAGAAGGGTATACTCGAGGTTGTGACCAGTGCACTGAGCCACATAATATACTTGTGAACAACCCGACTTTTACAGCCATCGGGCCATATTGCTGTGCATCAAATAATTGAAAAGTCCTACGTAACGAGGCGCCCTCTTTTTTCGCGGCGAACTCTACGATGAATACTTACACAGGAGTCTGCCCAGATGTCCACACAAGTGGCGTGCTACCCAGGGATATAGAATGCTACTTCGAGCTCGTGATCGCAGACGTGGATAACGACACAGCTAATAAACTTAGTTTGGGGTACTAAAAAAAGGAGGATGCTTCAACTACGATACCACCTCCTACGGTAGAAAGTGTATGGGTTTGCTCGGCTTTCCGCGCTCTACTCCTGAAGGATATGCAAGTATTTACTGCTAATAAAATCCTTACCTACGATCTTCTTCTTGTTTTATTCGTGGAGGGAGATATTTAGACCACGTATGTTCTCCTTTTGTGGTACTACACAATAATCCTTATGTAGTTTTGATATCCGATGGCTAATTTTGGAGATGTGAATAAGTATGTCCTCGATGTAGTAGTTAAAACAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 14:54:14 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTSXBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :uTDATA :uʀDATA :u@8DATA :uDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~VLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS+P1AM?~BYP1RL?~=P1WD?~FP2AM?~OP2BA??TQP2RL?~KUPBAS??."PBAS??+PCON??;PCON??9]PDMFTPDMF+PLOC?~4PLOC?~0aPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%+SCAN@SMED SMLtSMPL%%TSPACAinSPAC+SPACAinSVER4.0SVER XSVER,,/Scal@Scan@TUBEH10Tmpr<UserphARphCH+phDYbigphQLcphTRphTR7This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTSXBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :uTDATA :uʀDATA :u@8DATA :uDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabftFVocfcFWO_GATCFeat//f4GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEBLAST~~VLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS+P1AM?~BYP1RL?~=P1WD?~FP2AM?~OP2BA??TQP2RL?~KUPBAS??."PBAS??+PCON??;PCON??9]PDMFTPDMF+PLOC?~4PLOC?~0aPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%+SCAN@SMED SMLtSMPL%%TSPACAinSPAC+SPACAinSVER4.0SVER XSVER,,/Scal@Scan@TUBEH10Tmpr<UserphARphCH+phDYbigphQLcphTRphTR@~A67OQ1{zK90plkm$48V^p$&:?wMB>-j%;U*E^h9i_Qw|k8 <.(P-S"EnKl/:5t9z"DEoh9ZzZW-W 7W}$wFTN]%jEX@u]YMAmB] ]ddc}PMfOl>o UekB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RUR6? sdWUg>|});B]M{kgUHU.]g"Q%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tr%,yPyN+8ds16;evr./b?Ay0]:jjMSDDl[zLTHAyK^\'^7>p"}$&}0eq7A]T,.] 2,\auB9*(K#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OLogXY@ZB 3O{ ~VJ(/on}sF{AR8=(V-OGC#!^MrrG]fQ.fuX){Q;|si xS]8q#b;XCTCk|7[ZIxpVowa:t"@>oW~!Eb4;,}|O[nYI.xv^$beP *"udrVLgJRv)1mfc4hAC?-4$&LkfG;7oF`|zvn6&pg;gjmMUwch[}k@_ajFoW~&WZ$>tSEH[!]fKOvx4G!?`T/Hf.X7GtX6|k.j,PCH-p}*4d#R:c@ZIIC"&;UXU8x[K"4#O2C.98xOrz6 ?63_baI23|7hhSc%bVm? 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