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  $$$,,,444<<ADCJKKQRRYYY___fffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5f8c329580144f93a4f4f6a16f1f163fSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_13-06_07333130Seq16795134877_134877mfagan96-Well%K1319_G7_Persicaria_nepalensis_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z '5Nt/R5q9] X>R^gstjeaV@kS>(uS0 zQ'lO8&)6H^z?esM(nYB( y_I5% 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XAWE^A[D@M<0=%F291<_&C7> ^$Z+Ta:'K=.R[GG)!M<@ FFEJNSXM;#J9WR L58S4Q)V2;+"S 1S9\PW5P;_+V+/ ]6R0C0=AP#$Nc!SMc./=8>*A $6/_^.#JV ?P: G?ET @WRFP:V2XE/B> +;<^L__)XPZ+/QBY1_B@(`I AMHL+@M9* aB+--%X6:99+?VT1.2XCA)P,8MI;`$G%V\%*TZ2 U^X/(E8,( b Y&& *YK127I!? Q%6F1EP9 c_#H `VYE<F?!U;H]4e 8gwSRM+#"2lN^Wi?[6fudKz wD~'0P\!eE `mxZ!*~(rAh(JO0O( i"GCBiCk%!'cLq?U 'a=UsHegP4#J-;k]Mi -N'?d]e0uemOp;2xlB|!$AYc LITN6k.Pd=ZoRvWEjGc/ 7jk&nT/lAO"XE"N?*S\j UBmaV_Pt H ?dWk b *eD^aa5u Z T,4)_ ]!:^=mJ  YchSN.x#V'fjNLsi9 2Y M{bh  v .csZ{Vk~uvaWT`gO[SS+tWLL=$}GGR;76 ;.163:K0/!;,X0-<H2*0L063$ " ,)0Z3-&+0   !6I L) :O)M  * .4#3;.=!  ,  $.  0 6.!A'J!#3G"!"  '  $    (  "/%!  1-6A 2 19!2)"* !:"#& )"/7Z/3)8-+)%7=&G9F3)>PPPU0OOU0TnmUCC-UUU8AL==InF,Ja@KS`gOBWWEP=WW7QVWGQ=7#EqKaZd77UB9 GWEWWWWOVWJaUjeeUMSv`SW__pUsYeeXee\poMvhca{nFodqqqPZYiNsrrqqqqrr}lvx|hcp rUyc|PprrrtqyrQ_XAcqqrrqrrrr'$pp,sJ11&{I=MGnyie7^*Zr#Xc$7 c{C1v`HvP.gTmdXF@  ,T A _Ed$aVQ.pp=dt^h\i,Z}"w`Y%P:)Op99vZ++6%k%vCaj sDDlyp[0EjpfxGh;2k_|%'b9&3#Wtppppp(p,]6=p[pvGp1dlK8Vp#pm dYSb_q*z@G.09:JT!Pl|7,}9lB@jXiL@kp{(%&L{npIe7}Q`;vJVx%i}(*%=u=H=q/&d@soS+x{*j4]PfCiA-zn5S<l\-5X+#q*o Z;JrIp?E"0*FM4dzR3$z&d[%k|Jv/>LDRx^0o"\G`6_(IPMu9opgGR`ED[n?aJ0#4&!2>:&C7UTZW>8R?RNS@K;DriqcpOM:zCv"/PErsQ9nweVE*P]`=d>u\-{-di&BkT.6xVTC 1GAAAAGTTGTTTTCTCGCGGTCAACATTAGATAGGTAGCCACATGATACAAATACTTATCCCCAAATGGACGTCAACATGTAATTACCAGAACTTTCATCAAAAGGGTTTATTGTGAAGGATCCTTAGCCAATAATTTAATTACTTCTTCCGGAACAGACTCCAAGTTGTTGGCTTGCCCCTTGGTACGATCAAGGGTGCTTAGTCCATCGGGTCCCACAACCTGCCTTTGTAAAGTTGGAGAATCCCCAGCCTCCCGAGGCCCCGGCTACTGCAGCTGGCAATCCAGTTTGAGGAGTTCCTCAAAATGCAGCAATGATATCACGGCCATGAGAGTCAAGGACAGGAGTAGAATCGGTAAAGTTAATTCTATTAAACCAGCCTAGAAACCCAAAGCTGCTTAAGACTCTCTGTGGGGGGGCAGCCTTTCCAGTTGTATACGAAGTGAGAGATATGTAGAGCACGTACTCTGGCGGTGTATAGTACTACCCAATAATGCAACTGTAGTTTTAGTCTTCGATGAGCTACTTTCTAGAGGAACATATGTATGTGCGCTATGTACAAGAGAAAATAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 14:54:02 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtXASPtXB1PtWB1PtWBCTSd'BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <`xTDATA <`x͉DATA <`xFIDATA <`x DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~bLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS7P1AM>|NpP1RL>|IP1WD>|RP2AM>|[P2BA>>``P2RL>|WhPBAS>>:BPBAS>>8PCON>>GPCON>>ExPDMFTPDMF7PLOC>|@PLOC>|<PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%7SCAN@SMED SMLtSMPL&&TSPACAiESPAC7SPACAiESVER4.0SVER dSVER,,/Scal@Scan@TUBEG7Tmpr<UserphARphCH7phDYbigphQLcphTRphTR;This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtXASPtXB1PtWB1PtWBCTSd'BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <`xTDATA <`x͉DATA <`xFIDATA <`x DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabrFVocrqFWO_GATCFeat//rBGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~bLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS7P1AM>|NpP1RL>|IP1WD>|RP2AM>|[P2BA>>``P2RL>|WhPBAS>>:BPBAS>>8PCON>>GPCON>>ExPDMFTPDMF7PLOC>|@PLOC>|<PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%7SCAN@SMED SMLtSMPL&&TSPACAiESPAC7SPACAiESVER4.0SVER dSVER,,/Scal@Scan@TUBEG7Tmpr<UserphARphCH7phDYbigphQLcphTRphTRZsbJEP'WZ]FO{7I{6Emc9_lN#j`KJX?-'?C,F4te?t;t*2]>57_X~GjW<72s-@M+9mW'>YP~jM.9Wc@>(Jt6%> :L-Ev!$V@AK1ql{]j6K8eX)GE#!#+X(fcT%GY?tY%%\2D>HM6Fnf%)q.i.]X"vQ*m2W?p<;RAX(pAjw[~W@0$.9@_05a=gHFhwD~ ')&o*5V3"WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$b!;|dxU[YCC/3[`kI ?"U27`N`3[)|$X[@5+UTmHP$ c T-yh]}_?7KFN$@i(LAF;LsqBlC4hlTI#daC.?bFW&a:OcPX*F8BjhsDgn_RJ4ir$GE1Xz.?e Ml1}ssb[l"G>=?]p&k6v)ukO"yA&d}8qQgE5]U'j*-[~!Tq*nEg8Z~KI'_26?^V$Yeaejp~{>ZHm? >#veu]@|Z5hf6]X?K90#qN8>o XS_xP=O_l;mq;;9VgO(_y"`k:!.tkSY4E2 (# +6hjHepTey{'Gk>_FXWKr)YW [xinJil]ZCd]'DZNu@v0kc "V&S$~=}F)h>;-T)RJTZmU)P? sIe]Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkSOj!Q4aK{WlVS23|jyHAj}W2yv!)QBm#oCu"AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtXASPtXAUDTB1PtWB1PtWBCTSd'BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA <`xTDATA <`x͉DATA <`xFIDATA <`x DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabrFVocrqFWO_GATCFeat//rBGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE3LAST~~bLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS7P1AM>|NpP1RL>|IP1WD>|RP2AM>|[P2BA>>``P2RL>|WhPBAS>>:BPBAS>>8PCON>>GPCON>>ExPDMFTPDMF7PLOC>|@PLOC>|<PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%7SCAN@SMED SMLtSMPL&&TSPACAiESPAC7SPACAiESVER4.0SVER dSVER,,/Scal@Scan@TUBEG7Tmpr<UserphARphCH7phDYbigphQLcphTRphTR