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  $$$,,,444<<ADCJKKQRRYYY___fffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e36229416ff4418dacad4dd0a6c1a131Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_13-06_07333130Seq16795134877_134877mfagan96-WellK1319_E5_Solidago_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z:@IVgy &+,+)'%&(+/48=AEIKKIGEFJQYainpojaTD2 ycL5  4Qr9SmpZC. 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AMJ#@B9QP7$+8!_*b6(`O)R6/"F4TN G2Y_2>\A,B5) 2.1cO"O!@C-0T4&] V' XV-"VU+>DGRG_0bE;BIOC.F# ;M2)8L& JF#D5T8#d.@+^ I]!!HP C[ /4a>1=7 .?9ZTK 2@E@b5%LaI)- AJ#MK2;W(3. Q( X XLX@30D=BC@VCR-+c-XFO(5^bZR-D3 RN[%B>[OC8OI7P727>6IL =[G6I16O<; % I.6 AZO#$H;)bPS.Q $_ H6$62;_ 696):,R NbOH>4'E *%CZQE21<;QG8 6=UNX?;C6=QcA8+ZWM&9G ##N,]P@0PPC,1\@;PF2P<(` 1w+b`>AT+d@){/oW/_AS)<tSOqwn-;(HG  >;b%I ? ie-'=Cs ,5Sa"V5:)fBc]EghI3K\M'qy6?FpY0*H"dr0 .7[5okGUr&3=h 'a$0+Q}hzaK.k]8(+X~<PW( kvf|1\+Ptz Yhn+`y4>,&OI^N  <n1,jL%7`L]C"Jjd"W$T(/ko>]UnWE\lL%|y7~0VfTk_wQA]] 0=%#H"&~xv1IYnw {Y6oYH>QbCSh"48wuxhjg\YgLbiTYKe=F[d<:I>:DBGJCA74:/32#+!8#61+ (,'$-(..#%#'*&#7  %1!'$4#($+$!/$    % & " * #    ,  $    !  -( *(&% ) *8,>+92./0+-+ )"E6(7/)16!..L(D*09-+2$11@F>D&*U6@O78V5/GHA<G2GI<NHJ;0IH<1>>5fi<99</<4+2QXZA30[O`XH?UUI\>b>BGdN]^aQuQQQQOPHuu[[ggO;~<PYYd[[[[fjUiSaTaff;SdnmM||vkLYflolk\\Rrrriiii||yb\\S{)\]ii~yw|~ra||TiiX9||ir||||r!JipLpppag~ {S[C.c+,:H'wT+X%8LyP, :QCpCwGO7!p dl{.A%=0fD2dR1s>ck~}*:VR,6p)qVGH<pRA7ccs,dBpi+ ,fnFppn8W `p,pqpH89oT@^ +n4UKi #FW|~SPW9: >2 Pa4&dhT.#Q+>Q8= !.4*24I$/*-)5#%(3'()$. I>P%F3%($.$"+!Z'!- >''$)2==$21:!9)_#&)#$3+ ::5EI"!   0)').<G)+51(. +#6(!0.4%3.7 3!;8;3*!=&(!)-"!#(,$,+D''3( :P,(4#93%,=1$ 7.5@D0'##D6F1(GI ,,/$6#4=3-0<&2T 0?L2BVIO94"L7gV5)&"XOD.OACG8t"]:>P6)9_Fb3;O9" i/ Fa,)2U>&jgK:/5tkAA=*$FBFdBP>0y*.0TY-2>eW_1Q2K<^M8Y$<D0<EmHxNIv4J6S&&CZ;5<Stxf)CO;0&{vU.rz^w2D>m1xEgYq_>~7*):^I]o:S^=~"AAGATGGGTTTCTCGCGGTTGTGATGATAATATGTAGCCACATAATATACGGCCGTATACGGCAACCAATACAACTATATGATTGGCTTGCCACGCAGTAAATCACGATGCAGTGCTGCGTACGGAGGAAGATTCGTAAGTACCTCCTTAGTAACCGCTTATTATACAAACACCAACGAGCCCAGCGGATCACCCTCCCGTCCTTCGCACCACGGAACGTGACGAGGCAGTATCTTGTCCGCTTGAAGAGGAGGGAAACGAAATACCTACTAAGGCGCCCTATCGACCTTTCCCCCAGGAGGAAGGTGCGACACCCACGCACCCACCTACGAAACATATGATGTAGGTAAGAACGGCGTTCCGCCCGCTCCTCCTCTACGAGAAGAGATTAATGCCACCGTATATGATGCGACCCTACGATGGGCTTCTACGGTTTATAGGAAGTGAGAGATATGTAATCCACGAATATTGGCCTTTGGTGGTCCGGTCCAATAATTCATATGTAATCCTGATCTGCTATCCCCAACTCTGGAGAGCTGAATAAGTATGTCCTCTCTGTACAGGTTAAAATAACAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 14:53:50 -04:00AEPt@zAEPt@zAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt5ASPt5B1Pt4B1Pt4BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?^TDATA ?^DATA ?^S{DATA ?^DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~}2LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR?P1AM@hP1RL@drP1WD@mrP2AM@vrP2BA@@zP2RL@qPBAS@@TPBAS@@RrPCON@@b2PCON@@_PDMFTPDMFRVPLOC@[rPLOC@VPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%R7SCAN@SMED SMLtSMPLTSPACAeSPACROSPACAeSVER4.0SVER ~SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHRmphDYbigphQLcphTRphTR9This is the confidence range *ABI_Limits..FVocAEPt@zAEPt@zAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt5ASPt5B1Pt4B1Pt4BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?^TDATA ?^DATA ?^S{DATA ?^DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~}2LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR?P1AM@hP1RL@drP1WD@mrP2AM@vrP2BA@@zP2RL@qPBAS@@TPBAS@@RrPCON@@b2PCON@@_PDMFTPDMFRVPLOC@[rPLOC@VPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%R7SCAN@SMED SMLtSMPLTSPACAeSPACROSPACAeSVER4.0SVER ~SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHRmphDYbigphQLcphTRphTR-&/Ur1njEth@t#Rt+7+:W~TW<=?-O?;T9"MYMv'nE-\jC}RjJgi8fDtrWT 75Hya0#C"4kyE7*r;IYLDK2N<@O(LxC[>({]>R/Sao&w%:T?4ze^kQtAN-:d=iU~Hfzq?$y!TWrK96``^,Ffn@v-Gt>iHAj}W2yv!a!<}B]rQHfKbQS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;_?{-2Rmci0D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["#87zFl?xS_h{Vloc|7@4g-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW39nmG(l|I!KmZT[y i3e)/Pt=w6$.{y?hA(ue6bZ( ]'yZUogU7B!XwKcUW0gK >5(ePX!Hiwe2{sGi%ycX}OPmE$ww*{we*.l_pD8J)RnLHbcyShKn6g;X6YMFBj'~;H26t