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  $$$,,,444<<ADCJKKQRRYYY___fffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7bd1936d69b14dc8aee556ede6e29334Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_13-06_07333130Seq16795134877_134877mfagan96-Well'K1319_B7_Polypodium__echinolepis_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z <l?f"q S+|3^^ 6 { / ^ } i S 8  t 6m"R X!}Q-  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"*0342.*$  #.:IXgrz|xocTD6*    @A_AKADgKB.bcpKB_3730_POP7_BDTv3.mobtermGAAGGTGGTGAATCAAGCTGGTGTCAAGATTATCGATTGACTTATTACACCCCCGAATACAAAACCAAAGATACCGATATCTTGGCAGCATTTCGAATGACTCCACAACCTGGAGTACCGGCTGAGGAAGCTGGAGCTGCGGTAGCTGCAGAATCCTCCACAGGTACGTGGACAACTGTATGGACAGATGGGTTGACTAGCCTTGACCGTTACAAGGGCCGATGCTACGACATCGAACCCGTCGCTGGAGAAGAAAACCAGTATATCGCGTATGTAGCTTATCCTTTGGATCTCTTCGAAGAAGGTTCTGTTACTAATTTATTTACCTCCATAGTAGGTAATGTCTTTGGATTTAAGGCTCTACGCGCTCTACGCTTGGAAGACCTTCGAATTCCTCCCGCTTATTCTAAAACTTTCATGGGACCGCCTTTTTGTTTATTCAGGTTGAAAGGGATAAATTGAATAAATACGGACGTCCTTTATTGGGATGTACAATCAAGCCGAAATTAGGTCTGTCTGCTAAAAATTATGGTAGAGCCGTCTACGAATGTCTTCGCGGTGGACTTGATTTTACCTAAAGAAGGTGGTGAATCAAGCTGGTGTCAAGATTATCGATTGACTTATTACACCCCCGAATACAAAACCAAAGATACCGATATCTTGGCAGCATTTCGAATGACTCCACAACCTGGAGTACCGGCTGAGGAAGCTGGAGCTGCGGTAGCTGCAGAATCCTCCACAGGTACGTGGACAACTGTATGGACAGATGGGTTGACTAGCCTTGACCGTTACAAGGGCCGATGCTACGACATCGAACCCGTCGCTGGAGAAGAAAACCAGTATATCGCGTATGTAGCTTATCCTTTGGATCTCTTCGAAGAAGGTTCTGTTACTAATTTATTTACCTCCATAGTAGGTAATGTCTTTGGATTTAAGGCTCTACGCGCTCTACGCTTGGAAGACCTTCGAATTCCTCCCGCTTATTCTAAAACTTTCATGGGACCGCCTTTTTGTTTATTCAGGTTGAAAGGGATAAATTGAATAAATACGGACGTCCTTTATTGGGATGTACAATCAAGCCGAAATTAGGTCTGTCTGCTAAAAATTATGGTAGAGCCGTCTACGAATGTCTTCGCGGTGGACTTGATTTTACCTAAA!(1AO^u}%3?IUdt*5AOYdmy)5>JWcpz &0=HS^kx&3?LXft~(2=IVcp{ #/<HS^jw+5@LWdp}    & 3 > L U b m z   ! , 8 E R ] i v    ) 4 ? K W d q |  ! + 7 C O \ h r ~   % 2 = I T a m z   ,8CN[eq} $0<HS_lx+7DP]iv)5AM[fs~ &3?KXcn{ #.:FR^iu&1=HS[bmw)5BLXdp| '2>KVbo| #/;HT_ly $/<IT`lx ".9FR_kw!.;ER`lt+!(1AO^u}%3?IUdt*5AOYdmy)5>JWcpz &0=HS^kx&3?LXft~(2=IVcp{ #/<HS^jw+5@LWdp}    & 3 > L U b m z   ! , 8 E R ] i v    ) 4 ? K W d q |  ! + 7 C O \ h r ~   % 2 = I T a m z   ,8CN[eq} $0<HS_lx+7DP]iv)5AM[fs~ &3?KXcn{ #.:FR^iu&1=HS[bmw)5BLXdp| '2>KVbo| #/;HT_ly $/<IT`lx ".9FR_kw!.;ER`lt+   ## &%*#)'*&8 #* # '"" ""&"  3&,,+>;>>;>>>;>>>>>>>>>>;>>>;>>;;>88;;;;;;>;>;>>>>+;;>>>>>;>>>;>>>;>>>;;;>,;;;;;>>>>;,>>;>>>;>>>;;>>>>>>>>>8;>;>>;>>>>>8888.,>>//>>/>88/,888888>>>>8>8>>>>>>>>>>>>.>>>>8/>>>>8882888>8//'8>>>8>8/6>88>+88>>>>>>>>8>>>8>>>44>/>>>>>>>>>>>>464>4>>>4>4>>>)-04/444/146.4*.44.4::1:4:0:::6:6:64:666:::6::44!4:66*&:4)  :341:12:::016) '3:21::21222!.2&*)45,),0656::)*0*,0,26::21#,12::%4(4:)'"::121*2226666,/)'*1)65'2,*'-,)61(5(,1"%"3& #"   ## &%*#)'*&8 #* # '"" ""&"  3&,,+>;>>;>>>;>>>>>>>>>>;>>>;>>;;>88;;;;;;>;>;>>>>+;;>>>>>;>>>;>>>;>>>;;;>,;;;;;>>>>;,>>;>>>;>>>;;>>>>>>>>>8;>;>>;>>>>>8888.,>>//>>/>88/,888888>>>>8>8>>>>>>>>>>>>.>>>>8/>>>>8882888>8//'8>>>8>8/6>88>+88>>>>>>>>8>>>8>>>44>/>>>>>>>>>>>>464>4>>>4>4>>>)-04/444/146.4*.44.4::1:4:0:::6:6:64:666:::6::44!4:66*&:4)  :341:12:::016) '3:21::21222!.2&*)45,),0656::)*0*,0,26::21#,12::%4(4:)'"::121*2226666,/)'*1)65'2,*'-,)61(5(,1"%"3& #"J2V-'%/1T09`8=>,&#NL<J B)(V :O6)PLP,< $ FG1^-%`Lb4"'cSYD>0S/*X'6!UU<5.PQbc]K,$8D'Z0.V!3MZKOCJ&T_H ^)FI4CDIA)DQ.U13K= A, 0?K*7 K(' PC6-AEHE(Y1*?4GI4;QN 03XAA>#H51942?Z:$,)<#0D%G48[QBZW"$&"#I Q "Y>M@ E2:b47T9a` 3[<X(/0\F!<[5W 5Q=6aO"F;J5]\>VGLF=YEa"McNZQ%*JWRN/O.%Pb<KN4GT)LK7& VR738DS@+b(RB)BF'P\/XG`_ 7 U53C5-"*.M5FL?(L^ 23c@+=A /2J82,@$; @)RE8,0KY^PW5J8XV:)AP$S W3NG5)/56/V5P>V-0 L%\4J0"6QC!942`ZHF>C[^6T4#MZ2"[ _ 02L\35"" +&'Zf9XE_"dii"lZ44f*!)iOt qNi_k\)})]FOC.=~_O~7T Ai ]BM'uCbI-wmM` D s}LkR1ERua8upYSclSpVz]9,c}<6:3qJzZd B 82O(C%0xmq`CL&0 >8R.41L S-28gG%R\$0&f)3y4sTR-Id)-f_ZOubj$DzU;Xvb 3YaI! _ZQPwV/d:%jG\*Ar`ca>IH yG>QWtj@AfW{r\e&i.&AEkogLc&WEk U^]Uh?ReicH-gCn8UbhagKYVX@5]K6EH_^g7j36C<M?=(0:$'/C3;14',)A4I*(+#'>EJ#!  $#+$ )*2"-*1##-*$-.$$!,(# & '"-/&$#8"%,*%-# &.&4@6 +>#*'2!590,$26g60S'J#08!/-::K%"  --5H,/E-%'+"Z,$C6$3/-A+*(45(Q)(/(#I/S)f8`&I55I2nN>mKKsP9N:nDAcO=GUT5Dti6rSFRUGJ0YYYYLMAb`HSVVVEaG]eSSSSSOSSSGSSwmZc|ZdddlllfWdddffyddIdddTf?\xSGwKffxAxxh_A|iji]b``b~Mxxx_ooVjxvxhklh`````ehWoo}Ukhh^zx{Uuxxhsoosoo)*o`xxxx`BNx``op>;=P#iz,&dpdipp 0Hwp5a~yuiS3j\TTpVUg,o,(p)28RMjvpB0*v<x,pH[Mv8=9BpRdSo,dw Ddldp48}4!2  qDD8Tv8p8)"pdp~pp<V 6lLpZ@",p|>p;|+#pppEr' \ "$>k`/KC}iw+8-E WCP,O KiMhz3[$h"3o%CV7F#2% #=FE.G;$_sgV>+Rt kyV;ZAlckV6Y?6CMAL)?2-3* 3* ; $%2|@q0!LDD)%T8#OF?C1. @\+x>~@%Q+Ad=N(8,#HnV )*NQ &362F/3;,(F@)8r]?8XbA`=2 ?+!U7O/%+9%,40"2.49X,F',DCILKZ'YO2g]] U/2pF6;rnwW*14".8n3*H88 &BL=i+;89!#6468=`&LYD.&d?I5HJHH|MN>7B)<Oyj>J)E2@)/1o.b&?*r0T1P&WA-16\;87ts`TeJ3i  +YCt@;oDS=#&NE+-7V1QAQ)+2;|WL;fNowXp<PpZ*2$i}C/6N*5Gj]{;LFNdzapyn#*Q6\-^Qm~NP\yvRWs]e>Ja'uJ4;pw]AGGAAGTTGTGTAACTAAGCAGACTTTCTACTGTTTACACTACCGCTTCTTTTTTTTATTTGGTTGCCCTTCCATTGGTGTGGAAACATGGGCTTTGGACGGGGGGCCTTGATTTGCAAACACAGAAGGAGAAAGAGCCTCTAGCTGCAGACTAATCTTTATTAACAAAAACACCAAAAGGTACAGAGGCAACCTCACCCTACCCTTTACACAGGAAAGGCGGCGACTCCTTCTAGGGGGCCTTTGTAGAGGAGGGGAAGAATATATTTTGCGTGGAGCATCTTCCCACTCTCACCTTGGAGGAAGGGGCGAGAATGAAAGGACCATCTACCGCGGATGGGACGTAGGTTTAACCGAAGCTCCACGCGCTCTACTCGTAGGAGAAAAAAGGACTACTTGCACATAATCTGGGAAAATCCAACGATCTTCCCAGTGGATACTCATGGTGGGAAATATTTAAAGTCCCTATTTAGGTGGTCCATAGTCCTCCCCACTAATGAAAAGTTAAGATGTGTCTCCTATGGCTAATACTGGAGCGGCGTACAAGGATGTCCTCTCTGTACAAGTTAAAACAAATCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 14:54:10 -04:00AEPt@pAEPt@pAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt0ASPt0B1Pt/B1Pt/BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\'DATA ?\SDATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE=LAST~~}oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRCP1AMCiP1RLCdP1WDCmP2AMCvP2BACC{,P2RLCr PBASCCTPBASCCRvPCONCCbKPCONCC`PDMFTPDMFRZPLOCC[PLOCCVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%R;SCAN@SMED SMLtSMPL((TSPACAfWSPACRSSPACAfWSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB7Tmpr<UserphARphCHRqphDYbigphQLcphTRphTR%8This is the confidence range *ABI_Limits..FVocAEPt@pAEPt@pAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt0ASPt0B1Pt/B1Pt/BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\'DATA ?\SDATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabSFVocBFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE=LAST~~}oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRCP1AMCiP1RLCdP1WDCmP2AMCvP2BACC{,P2RLCr PBASCCTPBASCCRvPCONCCbKPCONCC`PDMFTPDMFRZPLOCC[PLOCCVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%R;SCAN@SMED SMLtSMPL((TSPACAfWSPACRSSPACAfWSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB7Tmpr<UserphARphCHRqphDYbigphQLcphTRphTR_R|!{VmMm[*&`cz"B``,_bX@sWFa}TYKt9>,3HB! Z c4K_f].r47'3QbIiEx~h6dYH.l_pD8Jq,./b _|(L{2TVyLyuiCgGz^n3HPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'21{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*MP4;Z4Lh2d?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!Kp8m|HtfpE}Ou>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["#87zFl?xS_h{zlCc|6g9g-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW39nm@%l7:I1~8U'W33EZ'nO$`j|"/WQ`Q`zX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmpD0,_03gPV]O8oe])%|2CWllme[OCy KJFU'.QHT/}]66{6sA bBT3 #pJEa#;{U,Z4Ei5TT3T&#ONx8DTR"S"E<\AEPt@pAEPt@pAPFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt0ASPt0AUDTfB1Pt/B1Pt/BCTS~BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?\TDATA ?\'DATA ?\SDATA ?\DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabSFVocBFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE=LAST~~}oLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRCP1AMCiP1RLCdP1WDCmP2AMCvP2BACC{,P2RLCr PBASCCTPBASCCRvPCONCCbKPCONCC`PDMFTPDMFRZPLOCC[PLOCCVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%R;SCAN@SMED SMLtSMPL((TSPACAfWSPACRSSPACAfWSVER4.0SVER ~SVER,,/Scal@Scan@TUBEB7Tmpr<UserphARphCHRqphDYbigphQLcphTRphTR