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  $$$,,,444<<ADCJKKQRRYYY___fffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d3a073b271ac4003a3ccdc89fa3c2a72Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_13-06_07333130Seq16795134877_134877mfagan96-Well K1319_A7_Indeterminate_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z/5AW{<_EwL,`2Ono`TI>3& nG_4aD*xv{Bt1_ OyrL!_0wh^\agljcYRS\jw}xhQ7 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"I{ tG& ?nuF$     0WpPLd 'U0   :igNRp Z2   7g-/tD!    @n ]5 6[nH)  ;d(5(c:   5YsUN^mD% 8[vO/    :boG(      "9UskRFMi jdr}X;(  0Fcz\C/!)AarQ6% !:[sN1  5WuM-   :]zU7"   -IjxX;$ (FjnO6#  1Qyj[[hmP7#  .HfqQ4 9WxdD'5SsiWOSbwiM4  -EareeqoP6# !7Rppb^ev{[?) 3LjeH/ ,>RdrzzrbN9% ";Xwt`TQYhzhN5 $8PixcK5"  +A[yaE/   (>Xt~qmtyv}wv|cJ4#     %7Ne{lU?+  2H`v}pd_`iwx_G1!  $2CVj||od^_hwu]E1! -AXn}kWD4& ,=N]iprmbTD4&  -<L[hqvumaQ?.  /BWmwaK6%  $+1452.(  (9L]kssl_N<+ %2D[vy]E4)#   @xA\ANuA"KB.bcpKB_3730_POP7_BDTv3.mobtermCGGGTCTGGGTCATGCTCGGTGTTAAGATTATAAATTGACTTATTATACTCCTGATTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCAGGAGTTCCACCTGAAGAAGCAGGTGCCGCGGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTACCACATCGAACCTGTTGCTGGGGAAGAAAATCAATATATTGCTTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTCGGTAATGTATTTGGGTTCAAGGCCCTGCGCGCTCTACGTCTGGAGGATTTGCGAATACCTATTTCTTATGTTAAAACTTTCCAAGGTCCGCCTCTTTGCATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTATTAGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAGAATTATGGTAGAGCAGTTTATGAATGTCTTCGTGGTGGACTTGGATTTTACCAAACGGGTCTGGGTCATGCTCGGTGTTAAGATTATAAATTGACTTATTATACTCCTGATTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCTCAACCAGGAGTTCCACCTGAAGAAGCAGGTGCCGCGGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTGTGGACCGATGGGCTTACCAGTCTTGATCGTTACAAAGGACGATGCTACCACATCGAACCTGTTGCTGGGGAAGAAAATCAATATATTGCTTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTCGGTAATGTATTTGGGTTCAAGGCCCTGCGCGCTCTACGTCTGGAGGATTTGCGAATACCTATTTCTTATGTTAAAACTTTCCAAGGTCCGCCTCTTTGCATCCAAGTTGAGAGAGATAAATTGAACAAGTATGGTCGTCCCCTATTAGGATGTACTATTAAACCTAAATTGGGGTTATCCGCTAAGAATTATGGTAGAGCAGTTTATGAATGTCTTCGTGGTGGACTTGGATTTTACCAAA'8BSbo&2@JXgw -8DR]hs #.9EO[gt!/;FR^it ,7BNZdq~'4@KVamz &2>KXdo{ !.;IS_jv  # / < H U a m w  " - 8 C N [ g t   % 1 = J V a m z  # . : E O [ f r ~    % 1 = J V a l x  !.9DP\hs $0<IUbmx !.8EQ]ht+6BOZgs(2>JWbo{ "-:EQ\hs&1>KWcp| !.:FR^ht&3?JWcn{ !+8DP\ht(3>JWdo| ".:FR^iv)5ALXepy ('8BSbo&2@JXgw -8DR]hs #.9EO[gt!/;FR^it ,7BNZdq~'4@KVamz &2>KXdo{ !.;IS_jv  # / < H U a m w  " - 8 C N [ g t   % 1 = J V a m z  # . : E O [ f r ~    % 1 = J V a l x  !.9DP\hs $0<IUbmx !.8EQ]ht+6BOZgs(2>JWbo{ "-:EQ\hs&1>KWcp| !.:FR^ht&3?JWcn{ !+8DP\ht(3>JWdo| ".:FR^iv)5ALXepy ( *%,##'"'%.25/,:*2)$4444::6.4.)>6>40*>>>)!>/8>)88>>>>>+88>88>>888>/,/6/>>>>>>>>6/>>/6>>>>>>>,;,1>>>>>>>>>>;>>>>>6>>>>>>>>>>>>>>>1>1>>>>>>>>>>,>>>>>>>>>>>//>>6>>>>>>>>6>>>1>>>>1>>>>>>>>>>>>/$>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>>>>>>>>>>>>>>68>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>4>->>>>>>6>>>>>6>>/>:660::*56:::::::::::::0::::6::::.26::64"  4:666:::::::4:4-::13::::33:::2:::3:16:))13:2:/:-#:2211::03323613:2:2:3:1/:230:).3)3':%!:5':6':2:4362::22'3:,/.*5:% 6'.6. -  *%,##'"'%.25/,:*2)$4444::6.4.)>6>40*>>>)!>/8>)88>>>>>+88>88>>888>/,/6/>>>>>>>>6/>>/6>>>>>>>,;,1>>>>>>>>>>;>>>>>6>>>>>>>>>>>>>>>1>1>>>>>>>>>>,>>>>>>>>>>>//>>6>>>>>>>>6>>>1>>>>1>>>>>>>>>>>>/$>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>8>>>>>>>>>>>>>>>68>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>4>->>>>>>6>>>>>6>>/>:660::*56:::::::::::::0::::6::::.26::64"  4:666:::::::4:4-::13::::33:::2:::3:16:))13:2:/:-#:2211::03323613:2:2:3:1/:230:).3)3':%!:5':6':2:4362::22'3:,/.*5:% 6'.6. - 80R#2:@,9@7V@>K49N+^aQ!3UX 7HcL78I:K+%*<RQW%/^-7; FD8b:5)!9:"E,^a;- AOM"7. ?c1MN,M_X[8La5;(<@Q&[G01HN>QKCZX(E[X^98GI(AEZ8+ ,.*X& K9N,W@DcTI 6CJ5D-c3>S4_+UF: [;:=[ 4Za-XRU Q2;1P\6&SHC  $4, W 3B`G9E-)c; JaY2R9H2bM@"F&RF+P1F$N#4 0C\] =BBI::^1M`dB`EB'2`/#R U.K5(8R.H<a<K\PT2+=9IGWF" Y+6THBQX"J< [[[:9?;6&]BC4GA F-;L@SE:J0 /_WO2> =WD,H.)a@M>%Q<_HML'= F#,DU"2(b*B 1!_O;3(9dMCI)/ UG#X^L>)O[_// X;$]#>9<SSMC=C=M\'Z5!/9-  2Y` /C L A/c+22/,bQ )>J3I='(2`-G=+92( #f7?<&DDykyZdp@~Lp9 uY5:%Isa A*35NO&gm$']hXCnmHJcKBV6 G,AZ-l?l&4Xc=1({{pLiL.Up(@yTOe1<n)0m:tf |b)#q"(^z 5J~EJ:puO\nk` o &zI(jw YK-i=7#~\wJa rfQ=<>r'A#&KWEf@S=sXNVK 8Hq),WA}6n~bV<F L  UymhjVe<|~ lo{@ OsmMgg9PwP^@ZMM3${sa{k4vx6RV(_->e9  H>C J Ig_]wLi0HKo_^l_`jmvlShSKfF[[hJDVM:E==407--+)0.( ++' .&"&    #+ $/                    #          ( #'+))"%*%(,*'"")3''")0'(440*K-:5;4I,+2;55:%5:941#)*E%88.5@C92.%252+.E13'DDDD=612:NgN3@@GTQOP<I<<<<NRZNRShJ=@FFGEQOKCSIHSYCIZe]XZAVIXhaGSSYbb??WKZ^^jdE]]\fTIiihrk??Vovjj^v??Gfqq\bdZoiYToe]i~geYkttm`iitthiiiihBhhu>pJ-("z,prmdyvA-9S8 *ra&5"qv8p,?$m8w ,,J0DMi7g?&u5>OP0w[5]3D9Yxp8,E-?vo<&B8@p~ W\mddZUhki|e5TNpH*p@Tya2p;&,1p,h8p6p()Aap-ppep0,z a$ sn`v/A@`_M67L-y_[L;;'SY}V=7Buf>/>?=nMR4GFQZX$Y7B\g7V[jehat`*9!##("*R%.0F:*+5 ! Q$ %$7$0&+"!*"-+-$&/ 1-%. '}F<G &;)3 *%0&)$577YF"+$)+1 ")6,/2B/.!(25& 31('*!='""#42+$,$$$?9"=+<CB)+'+$++ */-,& ?2/# /3&"3$$!%*6)2%."K;'F&Nl93(+G'(*.2? P<;_W<//!!&$- 26?0,6)(1&BK0 )2LLs*." 32OT=$3$@<&8@2-kv&IH^-3MW2I2 EN]B`.;!C2- ?Q1:Z0I=]>K7=- kHnR@t-X{Qd*HNgG<MiUS[9Zxn'ubcSCz>N6RRTo|iAAAAGTGTAACTCATTAAACGTGATGTTAATAGCTAGTCAAATAAGCTTCTGACGAATATTTCAACTGCTACACTTATGTGGTAGCCTTGATTTTACTCAATTGATTTGGATGCCCTACGACAGGAGGAATTTGCTACGATCGTTCCTAATTATCATCTAGAAGGAGAAACACTTCTGTCCCCAGAGGTCTTCCCGTGCCTGGAGATTAACAGGGAACAAGGCTCCTACTTGTTGTTTGCGGTGGAAAAGGAGGCTAACGAGATACTTGGGAAGGCTCCCCTTAAAGAGTTAAAAAAGGAGGATGGTCAGAGAAGTACAAAAACTCCTACAAAGGATAGTAAATAGCTTTGATCCCCGTTCCGCGCTCTCCTTCTGTAGAATAGGAGATTACTTGCACCTAAGAAAATGGCAAACTACGATCTTCTTCTAGGTTTATACGTGGTGAGAGATATGTACAGCACGAATATTCGCCTTTTCTAAGAATATGCAATAATGCATATGTAGTCTTGATATTCGATGAGTAATACTGGAGAGGTGAATAAGTATGTCCTTCCTGTAGAAATATAAAATAACCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 14:54:12 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtFASPtFB1PtEB1PtEBCTSXBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :uTDATA :uʜDATA :u@tDATA :uLDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~WLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS,,P1AM@BP1RL@>_P1WD@G_P2AM@P_P2BA@@TP2RL@KPBAS@@.PBAS@@,_PCON@@<PCON@@9PDMFTPDMF,CPLOC@5_PLOC@0PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%,$SCAN@SMED SMLtSMPL!!TSPACAhSPAC,<SPACAhSVER4.0SVER XSVER,,/Scal@Scan@TUBEA7Tmpr<UserphARphCH,ZphDYbigphQLcphTRphTR9This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtFASPtFB1PtEB1PtEBCTSXBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA :uTDATA :uʜDATA :u@tDATA :uLDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabgFVocfFWO_GATCFeat//fGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~WLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS,,P1AM@BP1RL@>_P1WD@G_P2AM@P_P2BA@@TP2RL@KPBAS@@.PBAS@@,_PCON@@<PCON@@9PDMFTPDMF,CPLOC@5_PLOC@0PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%,$SCAN@SMED SMLtSMPL!!TSPACAhSPAC,<SPACAhSVER4.0SVER XSVER,,/Scal@Scan@TUBEA7Tmpr<UserphARphCH,ZphDYbigphQLcphTRphTR'%N`!2Fu4WyYZ(Wa:nuXK|+U17ujZj_!p-">R2`Yyb oJH.`Ep2=TgVSQ<}LgU!IZHp#)+KT[^[G^%E:"*l#qN8>o Ueh,9n5NJw=;SocJyw*6TdO{7I{4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/ud?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!-,'bF4$T9tR3#=cC0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4ZI,|Q|T{\K71P3;fTR[fRr;-6U@;EGCfQ&HQ$ 0603XXbLmX;9 |.W162Q$OEHO6z7B-_8Es{sMV^@}Ou{,]#9zqyetl~E+p3-]3:!h]R_s_beT 1"/"%)O~exg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2s JX0bt-] 5y$47y 4orKOR`{A,}@1%i`+|*#aB6:}rJl6d+lbf*yA=$I;i/cE`\3 *hGW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUGxP=O_l;mqy=Y`ge#cme/^i1>QD/N.6MfJO]LW:Pb@1)O1M]^2nrD5pY-7cRb[8)FTM~j3XV=K35-x>;u4,ZBE=+HqdszeOf^DR!_T6#qLQ6?ZdwYc_`kc.H(- 7@o*.AQ>U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#_P1WD@G_P2AM@P_P2BA@@TP2RL@KPBAS@@.PBAS@@,_PCON@@<PCON@@9PDMFTPDMF,CPLOC@5_PLOC@0PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%,$SCAN@SMED SMLtSMPL!!TSPACAhSPAC,<SPACAhSVER4.0SVER XSVER,,/Scal@Scan@TUBEA7Tmpr<UserphARphCH,ZphDYbigphQLcphTRphTR