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  $$$,,,444<<ADCJKKQRRYYY___fffmllssrxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7e25038b01e54ebbb3f7953f2097ddbcSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-06_13-06_07333130Seq16795134877_134877mfagan96-WellK1319_A10_Commelina_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z .Da)AWj|&0:DMSSI4dAmS;%*GjGw2Z~b2 mXA( 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"3IavweRD=>HVfs{{rbN8$  -AUhuzvjXD1!  .BWl}~mYC0!  &8Mdx{l]RNS^lz|oaVPQXesyj\QOTap~sbO=-     $4FXgrvsj[I6&  %4DS`ikh`UKCAENZfospfXG6&  !.>LYbed^ULD@AHS_jqqk`QB4)  &2>JSXXSI=0$  '5BMUYWPF:.# '7HYemoldWI9*  (3=FMPPLD9-"  #1>JRVSLB6*  ,;JXcjlh^Q@1#  #).11.*$  (4@JPSPI>2% "/?Rf|iTC5*" -k@AIRAKABKB.bcpKB_3730_POP7_BDTv3.mobtermAGGGGGTGGATTAAGCGGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAGTACCAAACTAAAGATACTGATATATTGGCAGCATTCCGAGTAACTCCTCAACCAGGAGTTCCGCCTGAGGAAGCAGGGGCTGCGGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTGTGGACTGATGGACTTACCAGCCTTGATCGATACAAAGGACGATGCTACCACATCGAGCCCGTTCCTGGAGAGGAAAGTCAATATATTGCTTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGTTTCAAAGCCCTGCGAGCTCTACGTTTGGAGGATTTGCGAATTCCCACTGCTTATACCAAAACTTTTCAGGGCCCGCCTCATTTGTATCCAAGTTGAAAGAGATAAGTTGAACAAGTATGGTCGTCCCCTATTGGGATGTACTATTAAACCTAAATTGGGATTATCCGCAAAGAACTACGGTAGAGCAGTTTATGAATGTCTACGTGGTGGACTTGGATTTTACCAAAGGGGGTGGATTAAGCGGGTGTTAAGATTATAAATTGACTTATTATACTCCTGAGTACCAAACTAAAGATACTGATATATTGGCAGCATTCCGAGTAACTCCTCAACCAGGAGTTCCGCCTGAGGAAGCAGGGGCTGCGGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTGTGGACTGATGGACTTACCAGCCTTGATCGATACAAAGGACGATGCTACCACATCGAGCCCGTTCCTGGAGAGGAAAGTCAATATATTGCTTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCTGTTACTAACATGTTTACTTCCATTGTAGGTAATGTATTTGGTTTCAAAGCCCTGCGAGCTCTACGTTTGGAGGATTTGCGAATTCCCACTGCTTATACCAAAACTTTTCAGGGCCCGCCTCATTTGTATCCAAGTTGAAAGAGATAAGTTGAACAAGTATGGTCGTCCCCTATTGGGATGTACTATTAAACCTAAATTGGGATTATCCGCAAAGAACTACGGTAGAGCAGTTTATGAATGTCTACGTGGTGGACTTGGATTTTACCAA *6A]n| +9DRaq '2>LWbmy $1>HUan{ &2=IUaly #.9EQ[hu  .9DOZfs+7DQ]hs&3ALWcn{    ) 5 ? L Y d o {    % / : F R ^ k x    ( 5 B N Z f r ~    & 1 < H S ^ j v    ) 4 A N Y d p |  %1<HT_kw(4AMZep| %0=HT`ly ".:FS_jw!*6CO[ht'2>JUamz ".:GS^hr|*6BN[eq} %2=IVbnz "-:EQ_ju+7BN[hs'4@LXdp| #0<JUanz + *6A]n| +9DRaq '2>LWbmy $1>HUan{ &2=IUaly #.9EQ[hu  .9DOZfs+7DQ]hs&3ALWcn{    ) 5 ? L Y d o {    % / : F R ^ k x    ( 5 B N Z f r ~    & 1 < H S ^ j v    ) 4 A N Y d p |  %1<HT_kw(4AMZep| %0=HT`ly ".:FS_jw!*6CO[ht'2>JUamz ".:GS^hr|*6BN[eq} %2=IVbnz "-:EQ_ju+7BN[hs'4@LXdp| #0<JUanz + )!#6% #!5:15:1 :*56)466:::::1$/#&'>>>66>>>)*>6>>/>>>>6>>#>>>>>>>>>>>61>>/>>>>>>>>;;>>;;>>1>;>>>;,,1#>>>>>1>;;>>>>>>>>>>>>>>>>>>>>>1>;>>>>>>>>>>/>>>>>>>>>>>//>>1>>>//>>>>>>>>>>>1/>>>>>>>>>>>>,,>>>>>>>>>>>>>>>6>>>>>>>>>>>>1/6>>>>>>>>>>>>>>>>>>>>>>.>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>:':::6::6:::::6:::::6:6::::6:::::::::::64/46(  ":66/66::::::::66'::::::::2:::63.:13:121/)+3:::::6,11+01:.::*::6::/:4::5)!3336:'366++:212:3:243:2411::+4)::*':223 (:*'12 '" )!#6% #!5:15:1 :*56)466:::::1$/#&'>>>66>>>)*>6>>/>>>>6>>#>>>>>>>>>>>61>>/>>>>>>>>;;>>;;>>1>;>>>;,,1#>>>>>1>;;>>>>>>>>>>>>>>>>>>>>>1>;>>>>>>>>>>/>>>>>>>>>>>//>>1>>>//>>>>>>>>>>>1/>>>>>>>>>>>>,,>>>>>>>>>>>>>>>6>>>>>>>>>>>>1/6>>>>>>>>>>>>>>>>>>>>>>.>>>>>>>>>>>>>>>6>>>>>>>>>>>>>>>8>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>:':::6::6:::::6:::::6:6::::6:::::::::::64/46(  ":66/66::::::::66'::::::::2:::63.:13:121/)+3:::::6,11+01:.::*::6::/:4::5)!3336:'366++:212:3:243:2411::+4)::*':223 (:*'12 '"A, D;>GD;"TFPX^*K- R-JIP[=/;O,<-@P`TD>XcB( 2#R ;#2"?O@=`XE/3 MM>/;B_@2PP\[:= +.BG;&=GR-9IY8M'6<TGXN%@CWBD@/*RJYG#!&c+!;:$8)J : NbKW]dN\I4:GVL= ('ZL ?D \=O6 E_"II-3% CITC@S^Z 8bJ'-.BI1W4$ DJ8B"DbM%A2^C-U +\I]cM@;Z L<04])-5E=B$VB+'> E@. !"8&W K!8b?O ;4+)^7U ^W\/$I>7',GIb7#>RDWI-])Y CK:I Y *TY(7*d:OQJ#8FJ=36<+5 4DJ1%?'<PJI-R+K,+W'4&,=6$5CE ! HbM/# LSE, %=J43 '2Y &8<KM=[&VY _IW8J8[a7W^A^d2 , >K )C#I+;S^5L^=+?5EK4D+$_ #(X[\.T4&5I LS%PJ"(r~#[fZ$lX~,'l; |eq3&&u ($DDV?S_/ G)V \{k8 1dm2@W iyI*UD2``(d*#/TWoQ  m~tmiX"V`Qy/?fUAv qCO&;2"}L329Djs[$E`Vgd X`A%J<>S}" rB@T|%+53N #%me.+x*I_$D #DQ:=!'vxM^s"2f& AR+^X:S-<}c2Menr(Eh1nC%tjOUt-%jrl rp 6C^o RCzuKSTYx.8o0N$kW% cld*? o hH= 04RD)o}FK[8hnsnc\macZRUVCVMb[McVI>8898A453./5+0))*&-(#%&*%1)&% #) (" #*                   '              .       +(%(  )%+'') !$#$ *(&0$)%,/2;269=0/7!J"'=+=737'3846$,.2"1F*I833%==599::::G17F.6BHHEE:WK:::>:EEAIWPX:9:NNND?V?<tEC<<cH|ENNm4[GO?STd[PyxqWF``b^OBKOYdR_GQ^b|ye`qbc{yxLfEazykrphaqwV}~awiies|vw_nTetyWcixJppS``ppf````fmkcgppXppjpspdVT8)7@Sd$Gd$t!pwvvD@Cx,8dE,aOJFe<,J ~C~)c;q4)i D,!|C8e/yN,Xga R,pXt} mppr8p7 F=p!>$apH`p[^ 2`p8RpJuJC ,lGSv-Q9o'3*GB/Gv6fZZW':@q|W:Z97@C,/7  ,,H& )#+( hYB" $'  *"  $ ''" !# WI&!= %4"6#&! 2 '1+**,!$!( $$!-1!!%/4',) 9%"")#  "$ *, "%/6 -%8(C3+ ,! !"5'"76)%4-3?(#0)= -$47/&'$4)-(!,(-C4"'/!5CC3N,)P3.@6%79&$:G "FA,;GmKY./Jf=4-.3!*+35>w0/, :P}4:Q5!#E;xYcYXK35#TJIgnH7/n#NDD39+pI</:B}C1F]W=YF"UhTE^lS@}<>G8r^-3QdPEZ B@_/;u=zrl^#SGAAAATGTATAATTAGCATGTGATGATAATATGTAAACACATAATATACTGGCGAACAACGGAATGGATACAGATATATAGTCAGCGTAATGAGTATCACTTCACCGACAAGTGCTTCTTCAGAAGGCGGAACCGGCGCTAGCTCCTGATTCTCCTCTTGTAACAGGAACAGGTGTGTGTACAGAGGCACACCCTAGCTTCGATCTATACACGGAACAAGGCTCCTTTTTCGAGCTTGTACTTGTAGAGAAGGGAAACGAGATAATTAAGGAGGCTAATCTTCAAGCCTTCAAGAAGGAGGAAGGTGTGATTAGGAGGTGAACTCCTTCAGTAGATAGGGTGGGGGTTGAAACGGCTTTCCGAGATCTCTTTCCGAAGAATAAGTGAGTACTTTGTGTTAATATTTGGGTTAAACTCACCTTTTTTATTCGGTATCTTCGAGGTGGGAGATATGAAAAGCACGAATACTGTCCTTTTATAGCCATACACTATAAGGCTTCTGTAGTAATAATCTTCGCGGAGCAATTCTAGAGCACAGAATAAGTATGTACACCCTGTATAAGTATAAAACAACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-06 14:54:28 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtPASPtPB1PtOB1PtOBCTS\jBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;gvTDATA ;gvˏDATA ;gvB]DATA ;gv+DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~ZLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS0P1AM?~FP1RL?~B,P1WD?~K(P2AM?~T$P2BA??XP2RL?~OPBAS??2sPBAS??04PCON???PCON??=PDMFTPDMF0PLOC?~90PLOC?~4PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%/SCAN@SMED SMLtSMPLTSPACAi"SPAC0SPACAi"SVER4.0SVER \_SVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCH0/phDYbigphQLcphTRphTR+This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtPASPtPB1PtOB1PtOBCTS\jBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;gvTDATA ;gvˏDATA ;gvB]DATA ;gv+DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabjFVocjFWO_GATCFeat//jGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~ZLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS0P1AM?~FP1RL?~B,P1WD?~K(P2AM?~T$P2BA??XP2RL?~OPBAS??2sPBAS??04PCON???PCON??=PDMFTPDMF0PLOC?~90PLOC?~4PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  RUNT 5 RUNT  1RUNT 5 Rate 0GRunN""JbS/N%/SCAN@SMED SMLtSMPLTSPACAi"SPAC0SPACAi"SVER4.0SVER \_SVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCH0/phDYbigphQLcphTRphTR]%A'on!w.*R@_aA+|1hygF8y?^qs?C!e2=c-{O YXpN}5Mzi5fI[PeK}d>/JJr=*0_fs_c&a~'S{&vT+8@/S&ofW)NK[>9`ht1A{QuU[PbI^n0 Dx6m}:)G*.Nr]0;a=bZ5hf61F;K9JX@{F`!{/GC3[^NHE,v*(]4]m9pfaX%\NzU4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/J>zN62d-I;4,,pCw|#O\mk*]l-u4|8e7s`]pPC,61h67{=Y`gI)_|_n?NhN{CwN1d^M?92xsgCTbKr:=uK>qc[j]/7<("Hf.Q*s`AtGHrd+v/,J6v$NUvc*xGZ]k'lCKOMQwKdLC2fo;LY&f&){t~1{[q?Eb,8`a)}qjM>PCNIG2gc*?7kx`rxPo"`dbB-8'i2{q6.>`o?OpOw!b)9OO)Yr[q?d4}XiGOHKR!hjqiH(Ii!C1"|Ql;eX;9 %t,=pWyoAN+$0>'[- 7@o*.AQ>U)P? sIRD8z{KJ[.z^`A(uKN73B!Hiw8X&L>o.B3@dSO/qg616B4#ky`[qDr_WG\|[S*.JJof6[[HhEg5{og]|_}e])%O1"@7hhSc%bVm? : wT_RuhGlB^PY6C}Afz@jXaMuF_QJO!GE#!#hmmfc4.=+I}S$oa$fU~oM6CqwToN668d#6Z~=}F)h>xgY5"KBv'o>s1HT&}J39OQJMeP> A(_GZE?RVuU,ZBE=+^k42g|5[S*.JUx2ffU"&6,2SayX,8JThnbi2dP?l~Wo]yi-IX/TI@tsRG; LNs? |KHO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX>V