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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR,OUVWWWWWUTTSRPOOMLJJGFDBB@><;977531DKKDDD___uuu %%%---444;;;BBBIINOOUVU\[[bbagggmmmsrrxww}||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1d629d1fe8fd4ea68f321b8c27224357Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-02_10-37_07233130Seq16780134785_134785mfagan96-Well#########"##########"#####"""##"##########"################################"############"############"###################"#########################################################################################################"#######"############################"############""################"#################"###################"""##"###############"#########################"#######"######"############################################################"#########################"#####"####"#"######"####################################################"#########################"######"################"###########################""#####################"##########"########################""########"########################################"#############################"##############"##""#################"##############################"###"#####"########"###################################################################################"####################################"############"#########"##################################"######"###"########################################"################"###################################"#"############################################################################################"######"""#########K1318_H9_Bidens_alba_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zHMVdw 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r+>b]8pCbi :n k K`f=h9=Y Pp<v=x,fE2u=$DUf_fe8k8>q*Bbb42N<*=pr/:0,#N7h*5>9&32A9'Nrx'4.5BQOg`>Q"4<a0XPIh7H)]e25 ;s@\-CFU2O]5a9[fY'E?.QMef<aMwS3*855vj0$BK =37X{kHQ@D5<BbPE85[6=VR;*HH4W"5G:dx5='>Ob1.=X(@CM.+$RclE=$mA=#=bS63,cA5J]@jWWB0 S5T#EJ(-;0F"qrV:l5&3*c$hH?eAG6<=3g5h<hW#44]/c9RXvX5;9?34'u3(6L@biiK39+s)gSGmi\2Qze:cGU0+dG+ lULA.Gc9,*:"w7V&76S<l+*%I]60-/6:;oe$\9/N0"IUFVTH<IQ[F]<CG093E2H )kL53QUFL5=%S@z'L%?nwK7/ft`;+nL[K*13<Z6^&"6$d6YnvVB)F&3GV0-URW,b;"515RI<74uuy/fJQpr@|dr~v]N,aa/$>AGGAAAAAAATAAAGTTCCTAAGCCGAGAGTCAAATTGTCTTGTATGTGAGTAGACTGAAAATCCCCACTCCGAAGCAGATAACGCCAACATTGGAGCACCAAATCGAGGAAACTACCAACCCGGAATGCCGCCTGAAGAAGTTTGGGCCGGCGGAAGCCGGCAGTCCTCTTCCGGCCTACAGAAAGATTGGATGGACCGAGGTCCTTACGGACTTCGTTTCTTCCAAAGGACCTTGCAAGGGAATCGCACCTGCATCCGGGGGGAAAAAGCAATAATTTGCTTCAGGAGCTATTCCCTTTCACTCCTCTGGAAGGGGATGGGTTACTGGCGTGGATACTTCCATGGGACGGACTTAGGTTGGGTATTTAACCCTGCCTGGTCCAAGTCAAGGAAGAATAGGATAATCTAATGGGGAAGTTTAATCTTACGAAGGATTTTTTTCTTGAAGCCCAATTAGTAATAGTTAAACCGACCAGGTAGGCGCGTTCCCTGGGGGGGGGTCCTGAATAGCCCGAGTTGGGGGATTATGCCTCACATCAACGCCGGAATCGGCTGAAAGGATTGTGTCCGGTTGTTAAGTGAATTTCACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-02 12:25:19 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSaBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;wTDATA ;wgDATA ;wDDATA ;wDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~_wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS3gP1AMOJP1RLOFP1WDOONP2AMOXP2BAOO](P2RLOSPBASOO5PBASOO3PCONOOCPCONOOAtPDMFTPDMF3~PLOCO<PLOCO88PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%3_SCAN@SMED SMLtSMPLTSPACAh1SPAC3wSPACAh1SVER4.0SVER `SVER,,/Scal@Scan@TUBEH9Tmpr<UserphARphCH3phDYbigphQLcphTRphTR;<This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt<ASPt<B1Pt;B1Pt;BCTSaBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;wTDATA ;wgDATA ;wDDATA ;wDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabo[FVocoJFWO_GATCFeat//oGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~_wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS3gP1AMOJP1RLOFP1WDOONP2AMOXP2BAOO](P2RLOSPBASOO5PBASOO3PCONOOCPCONOOAtPDMFTPDMF3~PLOCO<PLOCO88PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%3_SCAN@SMED SMLtSMPLTSPACAh1SPAC3wSPACAh1SVER4.0SVER `SVER,,/Scal@Scan@TUBEH9Tmpr<UserphARphCH3phDYbigphQLcphTRphTRK2OI*-V>yj6m}:)9`[?y[nvbeK]TM7l:2Fj62o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sbV_f7o,f9r;qBwJUo6;^UWZi7WeOzJu%~4%rIZHp#)+KT>:L-Ev!$V@AKM\8}48vc. 7$?Z2o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*M0JNbm|XMrDK"*.GHiJ(/\_'MChMoG;KMJ95nM,aVh6dYHp-Op;ZJLgS]jTj.uFk4W7|!y]18`'|_n?26.?bFWJQ:Ocl2[ALYZB VT@g:((R:fiWjaFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72Weetzu^h0|5Q1V??}@8jaFsA bFX97z?&qN^Fu*t&:_Gr9#(eaK|`Q@#=,`Pf)NKo-jE(_y"X_tXL ViY2]2rj&3d}7&I]N@c'v+gK >xPQ1V'.$b=}$A>AR8d}$7|:zNz~vdlc*+?_u4ZfoQe=N^tpups'MvK}7V,2{+w" 4*39nbeWo0<:LP@]Xi-]js3W4KnuPQh^>kij7!}WQ`QEJ9."G>}l)-l&T|*1fCy]_^ave%e0MSaywKB3x[f9;"Y+Ax8aPI^QJH:,ptJdQex-U[dPzFy^q3rjWcgDkrD5pY-7cRb4/k3);Nq_MY3RH0jZ*JKtPqp3s5ub(+gbk1^W7!_TcG,y"o-W2Bj:w`LJAU}WqD@~TlW+b@cT]`Aqh-YS2N97NR0<+OAN[!p?p5"?u?&.z*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt<ASPt<AUDT}nB1Pt;B1Pt;BCTSaBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ;wTDATA ;wgDATA ;wDDATA ;wDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabo[FVocoJFWO_GATCFeat//oGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~_wLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS3gP1AMOJP1RLOFP1WDOONP2AMOXP2BAOO](P2RLOSPBASOO5PBASOO3PCONOOCPCONOOAtPDMFTPDMF3~PLOCO<PLOCO88PSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%3_SCAN@SMED SMLtSMPLTSPACAh1SPAC3wSPACAh1SVER4.0SVER `SVER,,/Scal@Scan@TUBEH9Tmpr<UserphARphCH3phDYbigphQLcphTRphTR