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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR,OUVWWWWWUTTSRPOOMLJJGFDBB@><;977531DKKDDD___uuu %%%---444;;;BBBIINOOUVU\[[bbagggmmmsrrxww}||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 541254d9ebca4799a0c2647323512640Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-02_10-37_07233130Seq16780134785_134785mfagan96-Well#########"##########"#####"""##"##########"################################"############"############"###################"#########################################################################################################"#######"############################"############""################"#################"###################"""##"###############"#########################"#######"######"############################################################"#########################"#####"####"#"######"####################################################"#########################"######"################"###########################""#####################"##########"########################""########"########################################"#############################"##############"##""#################"##############################"###"#####"########"###################################################################################"####################################"############"#########"##################################"######"###"########################################"################"###################################"#"############################################################################################"######"""#########K1318_F1_Ageratina_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z 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XV(A89CGIC>E55R AUR)R`(% H/5#V\!<'J1Bd$bVEQ<R-ZH/<&I17JATEXNI+VL$>#^C?7'=Z?,\Z#)7=S\.YWV8N'#'\LPQ Qb \+WWSO!F7J%%6)bO"= b4BFQPOL1 E&0C@HLD#46_E8/H]F2;.T&T 3*6 C@ _`EW</*#T CB!186.Y  !EJO!K76=Y`5I %I- =P#[[< U:6U.YL'597?=1H7N6VX[&S?U!1R<OEV7= c<c]G#@AUc'ICb@"OGYc[a6A=N)&=.,G=>-*PCT9Y-Oc.(SR7QV 6 *S6A*0]'(M6OLW5P-YWV/a H_<V52/ VA />],5F(^N]]Q(2\d 7\a" LT=,:O(="K y A1yj0j Ojy$&u '5-E5y ZOhwi\rPQ 8j - ]k )/o{iGKD_>sU)g6* _W.O/VIccL H{  S IW M~(%&'[%J,g&W7\,Tr` *0@ mwY\s7v8, i + %XbDQ<I'}7I>J<r shG4g,^R6rRUvk inj 2f  =<:iFnM]5%KET&"qsSm7H /]tZ};=8h##nv|V X#Q/c{nfuYp{GGflr{S~ChcpBaH G ' t&}U N:PY wrIY0F {]_yqdaU `oQ+g\3[C_(y$2#$&% !:  #"  +"   /G %      4'#+,5+6I&!4!("$  &",D. =&+7-"9 5: ;"HB@$#!  /*8K :H)  433d<7c5%D$"-3$f$r$:$$$+$***&^$IwB9*'^%,[R%]7(O/AB!8m),MYkYJ*o,rH2|9Tdu'8p8dX , I/pi-+?70OLI86!23kgr!Bh8$p=@VHl-8,h2Ep >!8/d1Sl` vpd,DtpCHj+E!<)ihOcH`dwJ_H2 A*i]A/-{l>7Qpll 'K$Y4.`hd8@9ikQl;Hg SX <-iv'eSH4\<78F$SkLX<_u_MSEIg@e~e9SN\$\4L<34(?WZ:c9Y\]L%QcF0I5g~@c:ID:55*2faG(85CKX"5]7YFV`>CVGj8<-KnL@Jvf\ec$Jt'jRiZ1Yk&l-0ZA^34*j[h^'E);&VL'+J@sN64D,AH83EODL0:?PXMNGQC4;0kZV=<JFw ?{c94(S+W<L,*/F?=_+)JBU)jFXtSVUsQ;0m8:R@pR/FTFT[^oG^Q+4MPF6bUu"%S/Dq@`C,6]IWL,M_BNdg-SI+%;=%9YG35w<Z9$F)3]#5(of@%BDOH_NIe9#SC:=A]eA)JnS`)EVlkilik#er>,Cn=%%,21Se1+9UKE:J}EI)v$FsA?uKHpz}e,R|T0;dvEK&U<+E"b}'l;olV>jHJ3=6:E=6Ne<PS{_q^%};Q(Y4Y4[#^K KS,FGAGGAAAAAAACGAAGGTCCTTTAGCGAGACCAAATAGAATATCATTACTTAGAGTTGGATCAGACCACCGAACTAGAAAAGACCGGCCTTCGTGCACGAAGTCTCGCACACCTCGAATCCGGCGAGCCGCCCGATGAAGCGTGGGGCTACATTCGGTGGCAATACTCATCTCGCCAACAGGAAGAGTGCGCGGACCGGGGACCTAACTGCCTTAACTTCCTTGGAAGGACGCTGCCTAGTAACTGCACCTGCCTCCGGGGGGTAAAATGGAAAGTCTGCTTTAGCAACTATCTCATTAGAGTCCTCTGGAAGGGGATGAGTGAATACCCAGCGTCCTTCCCAAGGACGGAGGATGGTTGGGCATTCAGACCTGCCGTGAGCCCTGCAAGGAACAATTGGAGAATCTGCAGGGGGAGCAGTAACCTCATAAGGACGCTCTTCTTGAAGGCGATGAGCTAAAAGGGAAACAGACCACGTAGGCAGGTTCCCCGGGTCCCAGTTCCGAAGATGCCGAAGCGGGGGATGAACCATTACAACCAGGACGGGAGGGGCTCGATGGGCTGGGTCTGGGAGTAAAGAGAATATACAGACTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-02 12:24:22 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt5ASPt5B1Pt4B1Pt4BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?^TDATA ?^DATA ?^S|DATA ?^DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~~vLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR@P1AMQiP1RLQdP1WDQn?P2AMQwP2BAQQ|%P2RLQrPBASQQTPBASQQRsPCONQQbPCONQQ`YPDMFTPDMFRWPLOCQ[PLOCQWPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%R8SCAN@SMED SMLtSMPLTSPACAgZeSPACRPSPACAgZeSVER4.0SVER SVER,,/Scal@Scan@TUBEF1Tmpr<UserphARphCHRnphDYbigphQLcphTRphTR5(This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt5ASPt5B1Pt4B1Pt4BCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?^TDATA ?^DATA ?^S|DATA ?^DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabZFVocIFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~~vLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR@P1AMQiP1RLQdP1WDQn?P2AMQwP2BAQQ|%P2RLQrPBASQQTPBASQQRsPCONQQbPCONQQ`YPDMFTPDMFRWPLOCQ[PLOCQWPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%R8SCAN@SMED SMLtSMPLTSPACAgZeSPACRPSPACAgZeSVER4.0SVER SVER,,/Scal@Scan@TUBEF1Tmpr<UserphARphCHRnphDYbigphQLcphTRphTReZ>\&bD8d)sGi=&MQJ1rpJ=&F)9OHUkg;V<@Qn&;|>S8pu_Zj&[[Rq&!q)~'pEA({]/AT^ogqlp,D~lk;)+S8UXabCjC:AVVu>zKVJeoiQ]yw/b@j/.$I _|!{l29O[m[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{ce+bS_?Z d~)*$Fs{`jC52#GN3KHU;HBIc+75i@TH/JBcwZOHte?tH"D3|>oW~!.eHB,}sv&'Yj}4]'xP!t p7swnqR#$|Bq_7U9RbU9Za~Uc4_rf)Vu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_ K*G* NL19udb}WQzOb3]!p?p5"?uu&rz*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS:Q&:C!g61CfFc':Z^mDt4)Mz9n#m+mb~p7hR.8}'L-vHU5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,|`Dp>H,o}Qm4'p=R .*}Lq{F}u^zq+ `VsIRD8z{!J!OpZIBoz?&qN^Fu*t":Ey+D;UTw|]jp&;2isfMFoM/V/5`agv`k';_szT/0p/(ZU&g"=6W\!3[s?RJAQ>KN73o ZbCd]ipPv6~MN#tH*J*q~|H~?pVRI<672tX>PG"BXP2gX