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%%%---444;;;BBBIINOOUVU\[[bbagggmmmsrrxww}||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c7cacbd494be449d8db21131bf4114eeSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-02_10-37_07233130Seq16780134785_134785mfagan96-Well#########"##########"#####"""##"##########"################################"############"############"###################"#########################################################################################################"#######"############################"############""################"#################"###################"""##"###############"#########################"#######"######"############################################################"#########################"#####"####"#"######"####################################################"#########################"######"################"###########################""#####################"##########"########################""########"########################################"#############################"##############"##""#################"##############################"###"#####"########"###################################################################################"####################################"############"#########"##################################"######"###"########################################"################"###################################"#"############################################################################################"######"""#########'K1318_E10_Euphorbia_heterophylla_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7z}utzvibbhnrndUF92027<BGLPRSRQPPQTVXZ]agmruvussuz6Rn!@Yo_1tR2mYJ?73112233468:973/.049;92) 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B2=pH, &))'"   "&'%      =~3qU5$    =u Ty<nA$         "O"_q@~L)   'RD(/ [U*   4g$ITD~S4 =r/VdY8 1rbL+  *P5UZA/v^Z3    @zkq+.c~TiC* +T)DI9U5  1bHZP/c>$     $I|&GPAZ9"   8qi]^>)1a/ob_6    #IJ~VkG/   !!"#%'*.26995.&  !!                                                                                                                       \az@bAAQOA3KB.bcpKB_3730_POP7_BDTv3.mobtermGGCTTCAAAACGCCTCTACCGTAATTCTTAGCGGATAGCCCCAATTTTGAGTTTAATAGTACAACCCAATAGAGGGCGACCATACTTGTTCAATTTATCTCTTTCAACTTGGATACCATGAGGTGGCCCTTGGAAAGTTTTAGTATAAGCAGGAGGGATTCGCAAATCCTCCAGACGTAGAGCGCGCAGGGCTTTGAACCCAAATACATTACCCACAATGGAGGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAGGGGTAAGCTACATAAGCAATATACTGATTTTCTTCTCCAGCAACGGGCTCGATGTGGTAGCATCGTCCTTTATAACGATCAAGACTGGTAAGCCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACCGCCGCTCCTGCTTCCTCCGGCGGAACTCCAGGTTGAGGACTTACTCGGAATGCTGCCAAGATATCAGTATCTTTGGTTTCATATTCAGGAGTATAATAAGTCAATTTATAATCTTTAACACCAGCCTTGAATCCAACACTTGCTTTAGTCTCTGTTTGTGGGTGACAAGAAAAAGGCTTCAAAACGCCTCTACCGTAATTCTTAGCGGATAGCCCCAATTTTGAGTTTAATAGTACAACCCAATAGAGGGCGACCATACTTGTTCAATTTATCTCTTTCAACTTGGATACCATGAGGTGGCCCTTGGAAAGTTTTAGTATAAGCAGGAGGGATTCGCAAATCCTCCAGACGTAGAGCGCGCAGGGCTTTGAACCCAAATACATTACCCACAATGGAGGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAGGGGTAAGCTACATAAGCAATATACTGATTTTCTTCTCCAGCAACGGGCTCGATGTGGTAGCATCGTCCTTTATAACGATCAAGACTGGTAAGCCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACCGCCGCTCCTGCTTCCTCCGGCGGAACTCCAGGTTGAGGACTTACTCGGAATGCTGCCAAGATATCAGTATCTTTGGTTTCATATTCAGGAGTATAATAAGTCAATTTATAATCTTTAACACCAGCCTTGAATCCAACACTTGCTTTAGTCTCTGTTTGTGGGTGACAAGAAAAA(3>FNZcr )7CP[iv(5DMS]jv  -8FS`mz!.6AMYfp{ $1;FP\fs!.9DOZfr~ $0=JS`kx)6AMZfr~    ' 2 = I T ` k w    & 3 A L X d o {    * 7 C N Z f s   , 7 D Q ] h t    # / : F Q ^ j x '3>JWcp| ".;GS`lx(5ANZfq} #/;FT`lx+7COYer}&3?KWbo{ ".:FQ]jv,9EO[gt&2>KWdo} $0<GS^jv+7CP]ht*7AMZeq~)5AMX_ht(3>FNZcr )7CP[iv(5DMS]jv  -8FS`mz!.6AMYfp{ $1;FP\fs!.9DOZfr~ $0=JS`kx)6AMZfr~    ' 2 = I T ` k w    & 3 A L X d o {    * 7 C N Z f s   , 7 D Q ] h t    # / : F Q ^ j x '3>JWcp| ".;GS`lx(5ANZfq} #/;FT`lx+7COYer}&3?KWbo{ ".:FQ]jv,9EO[gt&2>KWdo} $0<GS^jv+7CP]ht*7AMZeq~)5AMX_ht   $22:1445:6*->>4+*>)8>>//>>836>>,*>>;>>>>;;>;.;;>>>>>>>>;1>>>6>>>>>>>>;)>>>>>>;;>>;;>;;>>>>>>>>>>;66>>>6>>>;>>>>>>>>>>>;>>>;;>>;>>;>>>>>>6>>>>>>>;>>>>>>>>;>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>;>>>>>>>>>>>>;>>>>1>>>>>>;>>>>>>>;>>>>;>>>>>>>>;>>>>>;>>>;>>>>;>>;>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>3>>8>>313>>8>>>8>>>>>>>>>>>>>>>>>>>>>>>>>288>83>*8>>>>6>>/6>>>>>>>>>>>>>>>>>+>/1>>>>>4>>>>>>>>>>>>>4>>>>6*>::6::6:::::6:::::::::::::::::::6:::-:::::::::-$:66666:46416(::::::00) &/0&    $22:1445:6*->>4+*>)8>>//>>836>>,*>>;>>>>;;>;.;;>>>>>>>>;1>>>6>>>>>>>>;)>>>>>>;;>>;;>;;>>>>>>>>>>;66>>>6>>>;>>>>>>>>>>>;>>>;;>>;>>;>>>>>>6>>>>>>>;>>>>>>>>;>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>;>>>>>>>>>>>>;>>>>1>>>>>>;>>>>>>>;>>>>;>>>>>>>>;>>>>>;>>>;>>>>;>>;>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>3>>8>>313>>8>>>8>>>>>>>>>>>>>>>>>>>>>>>>>288>83>*8>>>>6>>/6>>>>>>>>>>>>>>>>>+>/1>>>>>4>>>>>>>>>>>>>4>>>>6*>::6::6:::::6:::::::::::::::::::6:::-:::::::::-$:66666:46416(::::::00) &/0& L#:$c$H` E_HV>-/_;,B[E`6=Ob9V)11C9Bac & %? #"&^?F<70/*O%:2.0ML.b=44#%bX"PTL5c"V-]4,M$Q#B,0&%>54 0)DT<8CR/+- < >! */DSIF$!O,UT)1Y$VL,PO/*D/ BKENZ8B%M$\#[B.ZE>HU)J@&ISIbUF SMKNW?G[Da )788%=I;3^5B#0\*-YR*26 :26B')> LJ.RA1d  38`<;+6"AYW)'"X8 ,]+;C">B0'%[?<P?.IJ `] '7\U"<.[;GZXZ$J5[=K606B@X@=X1J@@R934,XHBL $QM4FWOFO93M2V)aZ:"X? +cY5IIZJM=::MHOM18C2*4O.T/2=K=1B15["O ;!S:IY,a !;/@$?U7BA+aH:A7HU9!]D)'A ]`M141^LAG= TE"<K</&#=@8 5B7U?FUPT'U__b0Z<bb  :5 ?$5`>vA=fbXF2YX' {uu)&^^C'g^26aIo~UZ!|<xqv2.\ 2:$?@\6}LA$ I@%Q-5C?Y nBWtz@[<$~% d_g'TKx E+~ngTY o`u6[>5L i1H78q 5EetUAD g W!*}}"Q%B3X68#L fd@X\nv99ui$UH2q'>G# e0naGM b=mf_Ycp}(zPw-W}eOCqj [|W*D5xBxwrW7XE ]HUCae)8  }o n (HS^,x u?uhjPvOJ55JCY?/6m0=BC3&.58$%C"               "     " !+76 0 2;(/'#.#.",6%/7$653!*.;5/6)E!%C!,-G&:K(2P4A+1C$)61D6I6U&. 010''z''''H6pip<Cd dI;Zd,E[;&I/$;>o&,m/p[dy OVJQ`L:l+pC=p8V`=3)Nr [mVM1gw,O](,Z#N Q:i]Y!{O8&09g8g~D+pNnC"!? 2L%j77 A p(V2bd)ip8%rI;0>d]u&,] d, dv3; RpZ(ud pA88pp8B8=+Npp6BRmszp=4dHNH &"Y~3hAwNYB5D@/7MD0+2#F#n+ e0. C22[VHJ)*t)1%$ 1)-PK)  (>'* + /4-56/B!&/8#,6)!#* D($$P9O* @#03,':</+ "<& "! "'4"<) &-'/!%!" C8A!#2;.'"#%%.*3024C",'M>9))#;!"$:3%$#*/5%/66"6@@1)I*< &I' #!&.:52D3'>%!O?:&H>1%%"I3'@"$%-75/0;8E%T/0\.RAB@2M-111*+9'77$S.220W-:*,.@ 3,#&/?L3:C%!!8$&")J28*&M.Dh)[<E "3%2*4,(*$- ;3$%034 & 7!+",'-B5,O99B'!9>E=0,(h+1"%c&')>,/=*!%,(*%(.3"38*N&63B:!<28q(+7PK+l8(fP:,!80=;?`n*ve }FXYAAAAAATTTCACGGCTATAGCATTAATAATAGAATAGCGGGACCAGAAAGAGAATCATTGCACCGAACCCGAGAAAGCGGACCTTCATGCACCAAACCGAGCAAACCACCAATAGGGGGTGCCGCCGGACGAAGCGAGAACGTGGGGGAGTTCGAAATACTCTCCTGGTAAACAGAAAGAGAGCGCGGACCAAGGTCTTAACCGATTTGGTTTTTTCTAAAGAAGGTGGCAACCCAGTCAGCCCGGCGTCGGGGGGGAAATATGAAAAGTTTGCTTCAGTAGCTATACTCTTAAACTCCTCTGCAGCGAATTTAGTTACAAACTTGTATACTTCAATAGTGAGGACTAGAGTTGGGTTTACAGCCCTGCACAGACAAGTTCAAGGAGGAATAGGAGAAAGATGAGGTTTATGTAATTGTTACTTAGGAATTTTTTATGGAAGCCGAAGACATAAAAATTAATCCTACGAGATAGGACCATCCCCTACGGCGGAGTCCAGAATACCCTGAATGGAGACATTATCCATGACAACAGTGAAGGAGGCGACAAAAAGGGTTGTGGGCGGGTGTAAGAGAGTACTTCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-02 12:25:25 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTS$BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?TTDATA ?TDATA ?TSsDATA ?TDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR#P1AMGiP1RLGdP1WDGmP2AMGvP2BAGG{TP2RLGr8PBASGGTPBASGGRVPCONGGbGPCONGG`PDMFTPDMFR:PLOCG[rPLOCGVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%RSCAN@SMED SMLtSMPL((TSPACAfSPACR3SPACAfSVER4.0SVER SVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCHRQphDYbigphQLcphTRphTR>This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTS$BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?TTDATA ?TDATA ?TSsDATA ?TDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocnFWO_GATCFeat//?GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR#P1AMGiP1RLGdP1WDGmP2AMGvP2BAGG{TP2RLGr8PBASGGTPBASGGRVPCONGGbGPCONGG`PDMFTPDMFR:PLOCG[rPLOCGVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%RSCAN@SMED SMLtSMPL((TSPACAfSPACR3SPACAfSVER4.0SVER SVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCHRQphDYbigphQLcphTRphTRKat)ecH<]O9Y}TU}xE_q-%Qvqfkv4)xJt;["*Kky#!#fn2lsa!ly^pd1v/gg61C`wWsV&&?-}U`aK|`Vc.O_l;5)F}oP5?z#`D5^Uv*0.w{WZRPLO2+6'UH[2g[jWcgnW6Obe"`dbB-Y@iGu2tZG+F7u2]Hn~+luKnPv@+Idp"iK][UcydLC2fo;LY&0]g0b) e:=Us1HB+{fU2sA>i@8/(8\_pzN02%B4O7yr[q?Ib\f.6u,fIwe1PpRZ36ECJBGmP;w+_9na<\us0@Dcf[6(F}z2g[jWcg;Z6Obe1Cd}Fp82)XaM~6>udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"a-cZ,f*F8B,}|O[nY?KPQh3f9bz)4r@:sQ0Gz[Yi~X$*RW8d7YPIyEbBV$603:m\! hkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}O!p8Npdo(ktnPkOpau_l_e5 }37Rgx|fv<\;=AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt"ASPt"AUDTB1Pt!B1Pt!BCTS$BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?TTDATA ?TDATA ?TSsDATA ?TDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocnFWO_GATCFeat//?GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~}LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR#P1AMGiP1RLGdP1WDGmP2AMGvP2BAGG{TP2RLGr8PBASGGTPBASGGRVPCONGGbGPCONGG`PDMFTPDMFR:PLOCG[rPLOCGVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%RSCAN@SMED SMLtSMPL((TSPACAfSPACR3SPACAfSVER4.0SVER SVER,,/Scal@Scan@TUBEE10Tmpr<UserphARphCHRQphDYbigphQLcphTRphTR