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%%%---444;;;BBBIINOOUVU\[[bbagggmmmsrrxww}||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 78d3ffa7b572400daf0f7dfac95c011eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-02_10-37_07233130Seq16780134785_134785mfagan96-Well#########"##########"#####"""##"##########"################################"############"############"###################"#########################################################################################################"#######"############################"############""################"#################"###################"""##"###############"#########################"#######"######"############################################################"#########################"#####"####"#"######"####################################################"#########################"######"################"###########################""#####################"##########"########################""########"########################################"#############################"##############"##""#################"##############################"###"#####"########"###################################################################################"####################################"############"#########"##################################"######"###"########################################"################"###################################"#"############################################################################################"######"""#########K1318_D4_Leucaena_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7zms}/DS\^]ZVSPNLMPV\bdd`[WTTVXXUOG>5.)%#! 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'030)   #)+'    ,yznwd"$C  ]C.`PUY#%!`'     O23{OjA    ; !]~7 #  S+FD .d)   ;&Jxn^y*zp7&lE|X(  (i4 J:R+/fYpi ,|v-&ef-WP+!cC&\YAs6    <,x* N3.0/( .pb Z1;y"po'eC/$   JAYtF+V"EK=+ MX+aL$  !"    9[A1(xB!G"yx#0j3 R t>D  Je1c|16<e`1        7YU[/ #Y?qk7k<   &+.-)$E9LkH97:;70( "$&&$!!%&#     :a!H> Sc8     4yBa }Q6$          +c`p!L&  VUN)K*   "UBn0c69trX'MrH,   (X;_xA     B(to+6-H6^.pErK3$ 3e;uN M'!P?bO*   (V9p'U.     >w1 ]x>ybM?2$ C~-&D;p_>)        $M<7P>I[7#  '4=A@>>BHQX\[VNB6( "$&(+,-.../.+&   !.=N[baYM@3*#                                                                                                                                                                                                                                                                #s7@;A#:A@XA?KB.bcpKB_3730_POP7_BDTv3.mobtermGGGACGTACTTCCCGCCTCTACCGTAATTCTTAGCGGATAACCCCAATTTTGAGTTTAATAGTACATCCCAATAGGGGACGGCCATACTTGTTCAATTTATCCCTCTCAACTTGGATGCCGTGAGGCGGACCTTGGAAAGTTTTAGAATAAGAAGGAGGGATTCGCAAATCCTCCAGACGTAGAGCACGCAGGGCCTTGAACCCAAATACATTACCCACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAGGGATAAGCTACATAAGCAATAAATTGACTTTCTTCTCCAGCAACTGGCTCGATGTGGTAGCATCGTCCTTTGTAACGATCAAGACTGGTAAGCCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACCGCGGCACCCGCTTCTTCAGGCGGAACTCCAGGTTGAGGAGTTACTCGGAATGCTGCCAAGATATCACTATCTTTAGTTTCATAGTCAGGAGTATAATAAGTCAATTTATAATCTTTAACACCAGCTTTGAACCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACAACACAAAAAAAGGGACGTACTTCCCGCCTCTACCGTAATTCTTAGCGGATAACCCCAATTTTGAGTTTAATAGTACATCCCAATAGGGGACGGCCATACTTGTTCAATTTATCCCTCTCAACTTGGATGCCGTGAGGCGGACCTTGGAAAGTTTTAGAATAAGAAGGAGGGATTCGCAAATCCTCCAGACGTAGAGCACGCAGGGCCTTGAACCCAAATACATTACCCACAATGGAAGTAAACATGTTAGTAACAGAACCTTCTTCAAAAAGGTCTAAGGGATAAGCTACATAAGCAATAAATTGACTTTCTTCTCCAGCAACTGGCTCGATGTGGTAGCATCGTCCTTTGTAACGATCAAGACTGGTAAGCCCATCGGTCCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACCGCGGCACCCGCTTCTTCAGGCGGAACTCCAGGTTGAGGAGTTACTCGGAATGCTGCCAAGATATCACTATCTTTAGTTTCATAGTCAGGAGTATAATAAGTCAATTTATAATCTTTAACACCAGCTTTGAACCCAACACTTGCTTTAGTCTCTGTTTGTGGTGACAACACAAAAAAA &,:DKT[dpx  (5BN]gt'4AQY_jw%1>IWes,9EMXdq~ $0>LVbmy &2@MXcoz  -9EQ^ku(6AMZeq~    ( 3 B M W c o z    ) 5 B N [ h s   $ 0 ; F R _ k v    ' 2 ? 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M.A5S[':_^ #`,F,]?a368!a[PY V-JF* (X99+6[O_31^;:[bI`:J%4W5&XQ0*K "!c_AC]-O:Sc5G3`Z9O\O0(5K+R^Tc ['X$RN#B3`/EA GSS'CWbV[%JA RTH- /d?7/``9O3[C`EM8=$=2 J[`6 ]27$B02SL,S V5K OTV;A 4]]XL&9:2N-ZF-HGd*Y/)AZ!1M07D%^D#U@a+,GX"@!^,K / G<4cq(.]p&Rk_u%tg6$L-( )[$3|b8xX@M0  cV*j[{l:]|xL./8 sfw5H~|^j/N|[9 x"cI$C^0Uhj{ @c/Gk;jR0|5I;cpaFPL@P o% !WS@+d|CTI?H5njM?YC-5`  j<Vleu.HD Y5 tv&`32 B%&JV2<}(  mr=:wb?yiR   j*qD,OzV CVbKXywsAe\N0(9l.yeIa CNA )Y ` J,h 1  -EP&sRXZ=  y~m^L=OdB\L:=p-G2$620/)6((!!!)(  5             $%H   ))+ 4)(L.!"0*&8 # 5% )$+A#!?;,!(=**$$#:B0>8&$0 %E>8+" .Gb]$H$$+$$$8$Sg'pUrr *Clk>F X8OI}8.8p(Xp.  ^|+w2YgJ6!%F~DPTVyp? 1bZE~b{+"YG"? `.*p00\  %Ep.p8p,A#mp{Vf ITCpYg[OOp)~dG,&N">; :`rd2{S?1WJGfD]~pZZ)Xwu@Up^G8p#U.Umpp@ppp3p8 sSpufa</,d) O<oM$vH+#O1;+aANccf,E]t$) >0: P;{!fS;!!"Dl>P+.,%v%# $#4'()+Q08)#+-BC2,"'."(>l-"# 2+-2(*""" $%&A-( =N)3/'94+3(=#69D$+/ &24;@8.(6/J=45<2$D6"3,2.#)*"!*/G.%$!#6=,'8%" "!.)'+o%:K !HE%2%!K4'(!B8#$". &=%,- ;#-VS;.1?'A%F6(2A%$$?=- =%9@>$=\H&";,.I$&"9:$!HC=#/ "3M),-@<5$%3GE7*2.( P983@e0515)6&i:)"#,'#-(28+!9'9 !'!:*6:'>%?d3S#+0(PC*3!($-*%*=0'3/=w#/&# <!@g%=+(S8&2,35D<2g=,>"!4$.;:)%PI<\D*3P",8!Y5`r?&08;*/V69*2_IK8LC')m(aK2>0mYD |-w NVAAAGGAATAAAAAGCGTCTACAGCATTAATCATAGTATAGCAGGACCAGCCTGAGGATTATTACACAGAACCCTCAAACACCGACACACCTGGACCCAATCGAGCTCACCACCCAGGCGGAGTTCCGCCGAGCGAAGGGAGGGCCTCGGTTACTCCGAAAGGGTCTCCTGGTCTTTTGACACCTAGGGCGGCCCGGGGTGTTTACCCATTTAGTTTTCTCCAAAGGAGGGGGCAACCCCGTCGGAGCAGCAGCCTCCCTTAAAAGGGAAATAGTTGCTTTAGTAGCTATTCCCCTAAACTCCTCTTCAACCAGCCACGCGACCAACCCGGATCCTTCCCCCGGGCGGACGAGACTTGGGATTACAGCCCTGCAGGGCCCAATTTAAGGAGGAATTGGAGAAACATCATATATATTTGCAGGCTCATCAGGAAGCTTTTCTTGAAACCCAAGTAGTAATCTGTAAACTTACTAGGTATGGACGTCCCCAAACGCGGAGTCATGAGTACCCCGAATTGGGGGGTTATCCATTACAACAAAGGTGGATGGGACAACAAGGGCAAGCTCCGGGTGCTGATGTTATATCCCTTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-02 12:24:43 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?ZDATA ?ZSsDATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR/P1AMMidP1RLMdP1WDMmP2AMMw2P2BAMM{P2RLMrPBASMMTPBASMMRbPCONMMb}PCONMM`0PDMFTPDMFRFPLOCM[PLOCMVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%R'SCAN@SMED SMLtSMPLTSPACA^SPACR?SPACA^SVER4.0SVER SVER,,/Scal@Scan@TUBED4Tmpr<UserphARphCHR]phDYbigphQLcphTRphTR:This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?ZDATA ?ZSsDATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR/P1AMMidP1RLMdP1WDMmP2AMMw2P2BAMM{P2RLMrPBASMMTPBASMMRbPCONMMb}PCONMM`0PDMFTPDMFRFPLOCM[PLOCMVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%R'SCAN@SMED SMLtSMPLTSPACA^SPACR?SPACA^SVER4.0SVER SVER,,/Scal@Scan@TUBED4Tmpr<UserphARphCHR]phDYbigphQLcphTRphTRYxr$c>'_swW^$oVv+rrbf.f7y>i@UebYWg\I5Cd*9_krYZ=kZw4n,7]Ajci(l{~Gj`X0?888%g^^4tX {1t;#G)jp~0,W(YzXrDWy?0T4`FGN9\47.6,8efPmE$w^6Rwe7sE^Z{F9LwU$<;172+C~6bfm{l|?"wdp$^nPz/V3QEMa!1j"SD=;LgqK-o?7.Pv_*NsPB-N&W-dOsWpC@"i@3}cUV'zK\__KdrW*`)l@.u!n`%SzXbhe >k94uL'Jd"*+8@/S(`CW7;Kn|?I)_|_n?\h["#87zFlExSKh#zluf|{TBg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+;Np@YS{j4zW2`&TA]BGmb,tJLVudrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>Ube\W'KFkaqQltX[3>>B~:^gWiD!}%`8p39ma4yHR9MUE+LZ(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i,ZikPvypAX~L=?-XPKw+[_g4#q&N7u`GcO581W+V|$OX{w|uD[w61*$kXfj!yxF'iPr:/N2fKB_,qz7.5k|_MBSmv^>:.<)%,8EE.Om,P>bH6X128)TK7\4v3s'kY6C}B-mq5FaT2V3pVoqCH-p@v\vhsW__Z';fQGE-`x&iJ-,qzjN"o+Ax8amb,.63<%Ri24WF=-U[D~TWY+N@c'v+gK >o>s1H#"$w8@&!]T68n(0Ju*zv&1M2F{H/`WajZUTw|INi@C,6KP8)s5AVihx_7&OMb=#w&bN"@7hhSc%bVm? S8UXabCDkS4NRY0GbR31}ssb[l"G>}O!G,lwdRadJsV&1ZG|JW+eT`Vcyu3Be%{M*A#t5#r~^;?2p$MXAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDTB1PtB1PtBCTSBufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?ZTDATA ?ZDATA ?ZSsDATA ?ZDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabFVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~~LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR/P1AMMidP1RLMdP1WDMmP2AMMw2P2BAMM{P2RLMrPBASMMTPBASMMRbPCONMMb}PCONMM`0PDMFTPDMFRFPLOCM[PLOCMVPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%R'SCAN@SMED SMLtSMPLTSPACA^SPACR?SPACA^SVER4.0SVER SVER,,/Scal@Scan@TUBED4Tmpr<UserphARphCHR]phDYbigphQLcphTRphTR