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***>>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR,OUVWWWWWUTTSRPOOMLJJGFDBB@><;977531DKKDDD___uuu %%%---444;;;BBBIINOOUVU\[[bbagggmmmsrrxww}||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a86f82a52f934eebafd4c1822ac3de99Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-02_10-37_07233130Seq16780134785_134785mfagan96-Well#########"##########"#####"""##"##########"################################"############"############"###################"#########################################################################################################"#######"############################"############""################"#################"###################"""##"###############"#########################"#######"######"############################################################"#########################"#####"####"#"######"####################################################"#########################"######"################"###########################""#####################"##########"########################""########"########################################"#############################"##############"##""#################"##############################"###"#####"########"###################################################################################"####################################"############"#########"##################################"######"###"########################################"################"###################################"#"############################################################################################"######"""######### 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]>0]/8[H3\+-ZuAy^=)ez-%bU$5S s%/IoXISY3|;#!#;lJr?|8D g`(3Y9G"63eSm^BZ BcnsN [/k(8o(4ukzB7gz~<DPK>/ ).v5f`&J_Czmc'3S[m5Mi-}l~x d@mpa/y.IJV Ay<d L/[ k&!Qtrfe 4H|W )ihux|M#r$\yU# nRbzD@`##4gEZ*~h6A}\1 y Om 7g7 kK5 Pa\H [^xonZvdq*Omefk}pBV+8V<2:G5c. .<"$ 69%:&          #>4     #  Y'bQ  0 2*, 6  F# &C 1v *!C/$!_%3.ELP #OJ,4@)\<5()2 0$_JA#" (5 <7"FB]'2+* 6SG<Jc.#S:29V;";*9"""9999"E;"}brwT[\}uZpVW7 H\p8N#Vdx`np/ pJgSrdC.^@8AH 9CP->vCmLv;629XPT2 G1KXatqdt?w$5>L,0Z6lpTn[Hl,8m8"ld2/]IiyH,8Z.WP4Q$mpg*L[pce%D]T0C\^2F#d[ J3dv.IS,wA]AC8 @t.o):,<&z`rq4K%8uRfM;p8p3j-h~8qtp,!?& q4,['dH$-Q # ePJg!B a8? @3wYcBxS2&iub@buF:SR@gaI}\UE{g*E9>\D[OA.^^4E>wlU8N_(<`\4/Om}^S0&3IGJi6Qp^Wjc`PQWe}Jqd"ZN0FT/mW9rS^,4EwCg^[M8F>-6m&3yU;#+:LY@K^sP=F\PM0yBG~,)Y8B>0=MI;PP*;DwfnkepV<V.OC9$;BumMID`Y$OJWI+V<^A=J\i`[5&_`7D?F4ODr>H@I<Idnu/dwU)qQplTfJ8{5?7;JN`4.TWy\lR{;^*u)fSxAMZ.#+RcJKF {Z)>UU5<QIsaH`gdV1CcKRYE)*O>zWBJKOIIWrhGSL@=Uw.j>8jW|?Kt@q^jWGS,H]HU[ohbg@YH<R;-f.Anb.F5Q*1F;{@hdLfTFy }?`YTGAAGAAATTCCTTTGTCTTTAGCTATTGCTCAGAGGAATAGCATGACCCGATTAGGATTCCTGCACCTTCCCCGAAAAGACGGACACACGTGCATCAAATCTCGCACATCACCCACGGGGAGAGCCGCCGGAAGATGGGTAAGCCTAGACTCAGGGGAAAGCCTCTCCTGGTCTTTTCAAATATTGTGTGGCGTGTGGTCTTAACCGATATGACTTCTTCTAAAGGCGGGGGCACCACTATCAGACCGGCCTCCTGGTCGAAAAATTAAATATGCGCCTCAGCAGCTATTCCCTTAGGCTCCTCTTCAACCAGAAACGAGAACAAAGAGGATACTTCCCTAGTAAGAAGGAGAGTTGGGTTTACAGCCCTTCCGGCCCCTCTTCAAGGAGGAATCCGATAAAACATTTGGTTAGTTTCAAACTCCGCAAGACCACTTTCTAGAAACCCATGTGGTAAAAATTAATCAAACGCGGCCGGACCATCCCTTCGCCGGGCGGCCCTTATACCCCGAATTGGGGGATTATCCATTACAATTTAGGAGGGAGGCGGAAGCGAAAGTCTCTGTGGGAGAATGAGAATACCCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-02 12:24:45 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?a~TDATA ?a~ӆDATA ?a~RHDATA ?a~ DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~{LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISOP1AMJfP1RLJbWP1WDJkP2AMJtP2BAJJy;P2RLJpPBASJJRQPBASJJPPCONJJ` PCONJJ]PDMFTPDMFOPLOCJY/PLOCJTPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%OSCAN@SMED SMLtSMPL!!TSPACAbJSPACOSPACAbJSVER4.0SVER }SVER,,/Scal@Scan@TUBEC4Tmpr<UserphARphCHPphDYbigphQLcphTRphTR81This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt B1Pt B1Pt BCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?a~TDATA ?a~ӆDATA ?a~RHDATA ?a~ DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabiFVocXFWO_GATCFeat//)GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~{LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISOP1AMJfP1RLJbWP1WDJkP2AMJtP2BAJJy;P2RLJpPBASJJRQPBASJJPPCONJJ` PCONJJ]PDMFTPDMFOPLOCJY/PLOCJTPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%OSCAN@SMED SMLtSMPL!!TSPACAbJSPACOSPACAbJSVER4.0SVER }SVER,,/Scal@Scan@TUBEC4Tmpr<UserphARphCHPphDYbigphQLcphTRphTRf\mci2aqN8>o Ueh,9n5g~Ri6(*24;x$}FNVUCs-113xT]o7U"y6^i_y>]y&m"o`^ kS\KQpYp&!gL-GI&7.{Mco6m~2HBIUbe1Cd}FxQZEZ,}4HUXYnuPQhoNePv7b{Wj2oFtXyy3@=?]p&)C^DZ=CHTA,h{|dsYuTF}wK[*oCfo;LY&p5S_3I|o6HvFHZ( g>}WqD@~TlW+b@cT]`Aqh-YKuNB\K/w_+peQo)Vq}<*x_[c|)nl>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.2AVsN&CSM7F=W.X}=1m)h>SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdO#rTi8B)$?SUS+x30}k |o[Z*lOt:/`CHQaWkQ}QH8EE.Om,K]yDWYhY8|kN_uw >xPo1Cd}Fp82)XaM~VpYow.mU5s%<(]R06`NV-[?|$}~?V'i~?RVuUi\j-=p\q_W-'Ic6XH[AVePX!Hiwe2{sGiSVqrj/.3]HCi%{1k :O[O~}<"&eG1VcyST/4f_7}?e_M%_|_n?\h =&e=1&!rE_g4YDI#%+AEPt>AEPt>APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt ASPt AUDT|B1Pt B1Pt BCTS}BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?a~TDATA ?a~ӆDATA ?a~RHDATA ?a~ DCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTabiFVocXFWO_GATCFeat//)GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~{LIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISOP1AMJfP1RLJbWP1WDJkP2AMJtP2BAJJy;P2RLJpPBASJJRQPBASJJPPCONJJ` PCONJJ]PDMFTPDMFOPLOCJY/PLOCJTPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  %RUNT   RUNT  RUNT  "Rate 0GRunN""JbS/N%OSCAN@SMED SMLtSMPL!!TSPACAbJSPACOSPACAbJSVER4.0SVER }SVER,,/Scal@Scan@TUBEC4Tmpr<UserphARphCHPphDYbigphQLcphTRphTR