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%%,--444;;;BBBIIIOOOVVU\[[abagggmllsrrxww}||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3af58947b42744759c4335ccb9776771Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-02_08-50_07223130Seq16779134781_134781mfagan96-Well########"#######"###""##########"########################"####"###"##"####""#"#######"######"###########################"########################"#########################"########################################"#"""##"""###################################"######"##"###"##########################################""######"###############"#########################"##################################################"####"#####"####"#######################################"#######"#############"##########################################"########"#################"#####################################################"#"####"#"###########"##################"#####"###########"#############################"####"################"###"#######################################"#"#"##"#####"###"###################"###############""#######"#############"################################################"######"#######################"############"######"##############################"##########"#######"############"######"################"############"#########################"#####"#"##################################################"##""###############################"##################################"######"##"###################################### 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X6]U!~&Z`i06(~b0 4R sq1unKZc^m!0%S@BS>" ),Q*R 'sjM)>Ro$K<t)"-E1I; Q5i?:.#7[ZaCj_ZK=UJ\H#@< /@>RHd*o:5GJG6ntO-`O"y =n| #k rR#]YAMK;u;8};`)":)6Cz(=m$hS^RGz; '2i\wi{].<i-f7dc6b- k&[4p?cRxj{MS`K4m#,x  Fmpd 3kP+ $ B^q hU K]FhG Nm5k&2C2i\O`Jc[;F@A.1(93.                         3  _G     "'/3&!#21+&  ) #!$."414:##%"V"=.$J>F('?0&9K(*$?M+558;'+7 6 M2C.QF/9H*89'}ppSNpg V1Q"8Xpw|%h{xpMiKdCHs =Cw7rv|Tczpj0N' a ~|a_4yYx pjX0H&pZvV5si9IYc;`p ]8(+,2tw4=PCpMO9oadY J*W_tc0vGQ[PpjFGN'{,("Muj p,vGZ(n1,8F>tdp8+.0SpY"\p.p!:\M<>ptkT;-,}WU j  [^^3].A}.1EG*!V]OL:Y(n2_u9bX5F6<p`B(V.*5>* .'3; 2(5.&H"W+:1$84*(0n8N!V5$0,2,#1B#-% 0?n850M+H/,$)#!"(#.8; ..:5. 8%346&C(1,K(9 *A!*2 6*B.-,T((M?=+;#:9(-I=A23]?4NJ2"LD7(4G&>1,3=+L_/-*8))%/-6%(SA(/%'B][{^F.4( D&+H-M3)4!- %9+$+-#4>'$!>1?06=D$5#=057433.(C#3("/')L,&T$27<95)C)='4:2V:&&BF'Vo%9\M-YT1-%BE:-.O(C4Z<.j-(=#4W1<7;,+*4)M.7nUV(F'-., 8,3;' dHAD%-N8>B?b+#Lc***CK#&-"$A%/q<-O>C0&Y(ZUR.7+FEFH_UD-21?`<.63'}25tE]Xi-e Z/mPOAGGAATGTGGTTATATCCGAACGTCGTGATGATAATATTTAAACACCTAATATACGGACGAATAACGGAACCGAGGGGGAGATATCGTTGGAGACCTTTGAGTCTCTTCTCCAACCCGAGCATCGTTAGAAGGAACAATTGTCGCAATATAATCAGTATCCTTTTATCTGACAAAGACTAAAGTGAAGAGAGACCAACCCAACACTCGTTATCGACACGGAAGACGAAGCTGTTTCCTCGAGGCCGGTTCTAGAGGAGGCCAACGGAGTAATGCACATGTAGCACTATTCCCCTTTCTCCCCGGAGGAAGGGGCGACAATGACAAGACTACCTTCAGTGAAAGTGAAGTAGGTAGAGTCGATGTTCCGCACTCTCTTCCTGATGAAGGAGCGAGGATTTCCTCCTAATAATACGCCAGACTACGAAGGTTTTATTATTGGATGCGAGGTGAGAAAGATGGAAGTCACGAAGACCGGATTTTTCTGGGCCGAAGCGGGAGGCCAAATGGACCCTGAATATTCGCTGAGCACCACCGGAGAGGACCCTAAGGATGTCTTCGTCGCTGTGGAGAGTAAAATCACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-02 10:37:15 -04:00AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?XTDATA ?XDATA ?XS8DATA ?XҐDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE%LAST~~}hLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMGhP1RLGd[P1WDGmwP2AMGvP2BAGG{!P2RLGrPBASGGTjPBASGGR#PCONGGbPCONGG_PDMFThPDMFRPLOCG[?PLOCGVPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPL!!TGSPACAd }SPACRSPACAd }SVER4.0SVER ~SVER,,/Scal@Scan@TUBEF5Tmpr<UserphARphCHRphDYbigphQLcphTRphTR"7This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtB1PtB1PtBCTS~BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?XTDATA ?XDATA ?XS8DATA ?XҐDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabLFVoc;FWO_GATCFeat// GTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE%LAST~~}hLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMGhP1RLGd[P1WDGmwP2AMGvP2BAGG{!P2RLGrPBASGGTjPBASGGR#PCONGGbPCONGG_PDMFThPDMFRPLOCG[?PLOCGVPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPL!!TGSPACAd }SPACRSPACAd }SVER4.0SVER ~SVER,,/Scal@Scan@TUBEF5Tmpr<UserphARphCHRphDYbigphQLcphTRphTRP7=4g@o!WFoe~8/zz-nX>Wc5O#|$@h27K^1FJ1)l]iRy$( &k4NC4kfJ-k4Ei5TT3T&#ONx8DTR" Wgw}kEOE!0z^n3HVQQEO==Q$b03XXbu6x+9Hgn+1T:o5Q*oc0c3}}1%jF}Esz3P<}u^ZVRiVdj>B~:^gWT<;u4#kBE=+Hqd-wvXfT;KBTAq;ah^.p2kSGKI'_2q@,p;YCWeq }X'KtFW.: wT_eD4<0|fP%4X+*flVS:3AGXSXW .C5wz^`,#W\m_WqDeNU%\2i$@o[%V[#m26yTA]Bp/p(dxRyvIKJ#G njco]ZV6Erq4ULv\?=&MmZY\&OIefaqQl'[)VBTd.u?z:6R;B*#k@l'Z9dEez x:H-p@~KCozPk&OE6OLpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfF{\)lOY }YWoa$1pfnST/4f!Z56N|&ZG90Si!AEPt?AEPt?APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtASPtAUDT_B1PtB1PtBCTS~BufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA ?XTDATA ?XDATA ?XS8DATA ?XҐDCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabLFVoc;FWO_GATCFeat// GTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE%LAST~~}hLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMGhP1RLGd[P1WDGmwP2AMGvP2BAGG{!P2RLGrPBASGGTjPBASGGR#PCONGGbPCONGG_PDMFThPDMFRPLOCG[?PLOCGVPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%QSCAN@SMED SMLtSMPL!!TGSPACAd }SPACRSPACAd }SVER4.0SVER ~SVER,,/Scal@Scan@TUBEF5Tmpr<UserphARphCHRphDYbigphQLcphTRphTR