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%%,--444;;;BBBIIIOOOVVU\[[abagggmllsrrxww}||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 397883305ccd48668ff0f35410a4f236Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-02_08-50_07223130Seq16779134781_134781mfagan96-Well########"#######"###""##########"########################"####"###"##"####""#"#######"######"###########################"########################"#########################"########################################"#"""##"""###################################"######"##"###"##########################################""######"###############"#########################"##################################################"####"#####"####"#######################################"#######"#############"##########################################"########"#################"#####################################################"#"####"#"###########"##################"#####"###########"#############################"####"################"###"#######################################"#"#"##"#####"###"###################"###############""#######"#############"################################################"######"#######################"############"######"##############################"##########"#######"############"######"################"############"#########################"#####"#"##################################################"##""###############################"##################################"######"##"######################################K1318_D1_Cheilanthes_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7zP 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R@AADhA ]KB.bcpKB_3730_POP7_BDTv3.mobtermAGCACCGGGTGAAGTGAAGCAAGCTCGTGTGTCAAAGATTACCGCATTGGACTCTATATACACTCCCGAATACAAGACCAAAGATACTGATATCTTAGCGGCTTTCCGAATGACCCCCCAGCCTGGAGTACCGGCTGAGGAAGCTGGAGCTGCCGTAGCTGCGGAATCTTCTACGGGTACATGGACCACGGTATGGACAGATGGACTTACTAGTCTTGATCGCTACAAGGGTCGGTGTTACGATATTGAGCCTGTTGCTGGGGAAGATAATCAGTATATCGCATATGTAGCTTATCCTCTGGATTTATTTGAGGAAGGTTCCGTCACCAATATGCTGACTTCTATTGTAGGTAATGTTTTTGGATTTAAGGCCCTACGCGCTCTGCGCCTGGAAGACCTACGAATTCCTCCTGCTTATTCCAAAACTTTTCTTGGCCCCCCCCACGGTATTCAGGTGGAAAGGGATAAACTAAACAAATACGGACGTCCCCTACTGGGATGTACAATCAAGCCAAAATTGGGCTTATCTGCCAAGAATTATGGTAGAGCAGTTTATGAGTGCCTTCGTGGTGGACTTGATTATACTACAAGCACCGGGTGAAGTGAAGCAAGCTCGTGTGTCAAAGATTACCGCATTGGACTCTATATACACTCCCGAATACAAGACCAAAGATACTGATATCTTAGCGGCTTTCCGAATGACCCCCCAGCCTGGAGTACCGGCTGAGGAAGCTGGAGCTGCCGTAGCTGCGGAATCTTCTACGGGTACATGGACCACGGTATGGACAGATGGACTTACTAGTCTTGATCGCTACAAGGGTCGGTGTTACGATATTGAGCCTGTTGCTGGGGAAGATAATCAGTATATCGCATATGTAGCTTATCCTCTGGATTTATTTGAGGAAGGTTCCGTCACCAATATGCTGACTTCTATTGTAGGTAATGTTTTTGGATTTAAGGCCCTACGCGCTCTGCGCCTGGAAGACCTACGAATTCCTCCTGCTTATTCCAAAACTTTTCTTGGCCCCCCCCACGGTATTCAGGTGGAAAGGGATAAACTAAACAAATACGGACGTCCCCTACTGGGATGTACAATCAAGCCAAAATTGGGCTTATCTGCCAAGAATTATGGTAGAGCAGTTTATGAGTGCCTTCGTGGTGGACTTGATTATACTACA *6BJS_kt{ '5ALU[ft )4@INYdlx *6?KWdpz %2?JVboz'4@MYfs~&1<IUaoy !/<FQ\iu (3?IUan|  " / ; F Q ^ i u    ' 3 ? L X d p }  " . : F S ^ k w    & 2 = K W b m y   , 7 B O [ g s  &2>IU`lx*7DN[gs %1=JVbo{ !.:FS_kv+5BOZfr~%0<GS_kw ,6BNZhs~(5?KWco{ $/<HT`ly +8CP\hu)5BNZer|&3>JWcny %1=JV`jw  *6BJS_kt{ '5ALU[ft )4@INYdlx *6?KWdpz %2?JVboz'4@MYfs~&1<IUaoy !/<FQ\iu (3?IUan|  " / ; F Q ^ i u    ' 3 ? L X d p }  " . : F S ^ k w    & 2 = K W b m y   , 7 B O [ g s  &2>IU`lx*7DN[gs %1=JVbo{ !.:FS_kv+5BOZfr~%0<GS_kw ,6BNZhs~(5?KWco{ $/<HT`ly +8CP\hu)5BNZer|&3>JWcny %1=JV`jw  *! &&6   *+! #>>>888/88>3/>1>/*;;>;3>;>>.>8;>>;;;>>;>>>>>;>>>>>;;>>>>>>>>>>>>6>>>;>>>;>>;.1*>;;>>>;>>>>>>;;>+>>>>;>>>>>>>>;>>;>>>>>>>>>;>>>>>>;>>>>;>>>;>>;;;88866>1>>>>>;;;8;;>>;>>>>>>>>>>>>>;8>>8>>>>;>>>>>>>>>>>>>;>3;;>>>>;;>>>>>>6>>;>>>>>;>>>>>>>>>>>>;;>>>>>>8>3>>>>>>>>>;>>;>>;;>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>;18>>>>>8>>>>>>>>;>>>>>>>2>3>>.>"+>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>8882)  *>6>>>>/8>>>>>>>>>>/6>>>>>>>>>>6>>>68>>>->>>>>>>>>>>>/6>>>>>>>>>>6>>/6>6>>>>>>>>>6>444+"'-::6:664(44': *4!0664/# !) !! "%6%   *! &&6   *+! #>>>888/88>3/>1>/*;;>;3>;>>.>8;>>;;;>>;>>>>>;>>>>>;;>>>>>>>>>>>>6>>>;>>>;>>;.1*>;;>>>;>>>>>>;;>+>>>>;>>>>>>>>;>>;>>>>>>>>>;>>>>>>;>>>>;>>>;>>;;;88866>1>>>>>;;;8;;>>;>>>>>>>>>>>>>;8>>8>>>>;>>>>>>>>>>>>>;>3;;>>>>;;>>>>>>6>>;>>>>>;>>>>>>>>>>>>;;>>>>>>8>3>>>>>>>>>;>>;>>;;>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>;18>>>>>8>>>>>>>>;>>>>>>>2>3>>.>"+>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>8882)  *>6>>>>/8>>>>>>>>>>/6>>>>>>>>>>6>>>68>>>->>>>>>>>>>>>/6>>>>>>>>>>6>>/6>6>>>>>>>>>6>444+"'-::6:664(44': *4!0664/# !) !! "%6%  BL#TDEI<EB,"F@AV5VB38K(,I.1OJRT&_ G^+@:Y/$c(:-9?1*(#>0I"<:ZKbR#X).d6 5-)-)WR .I:6Q44 G+'[45[*(\.Q2J'A`#L:d /b-[E*1,`I_0/SY]:K4K.+ 0J=F3G1[-!X;")8PTS )A9!-&X.A/[E=U)+ MD"43!0$)( &7Q3A::V`3J!U49K`'8)14)]> 60N^%TP"&d / :N]S8Abc :$YG-#cD; ,a4] HWXE>^a!(NA`%D"L$_ -:OR.UCQOZP ?5E^)_:^410FV(_<'2Q84a!)O]FY_'CMW 2*FXBK" PRL !SMUdNBc7FJ>SIR,'M$P (D=F1  CW?JI:5 EG4;_DD8+c>C\8Z:Z*[ S!HI]Y+H!^JL0CA&X,99 ?a\E.M6 a3S$.>_]@" 2_4 _?Q\W52`@ 1'GA_aHP>^N .]DK= -/jU XB}N A M i-cVRde7=8iq )bBeuc)J]qeL;1abMA{R&iU`g.3?TINnXQB<Q@6 NWxvq kfvZ'OMk2z}u|yMX^<\c<H*27!g#6 Ujc!eKc&,7;;`6RMg}yAeKA_-]rC3P*QZ*<J/p*N7ON0(NB+FP`#SKn=lD&K{TUf^'?N j_M-3K{R]c2YyZD]l*ox  %&N,O{1R5Cbmh@:"{%!V5#uFIb cV; %-W1 Ps0 HF H 1&p+=\. ZxrkIOnNkR^<M&NIBOC'"^91PB / "  '          "C    "    %&  *)( . /$+(+3"B$ * .    A>*%j")0D*&#"7 <B%!:)#^<5%_0.4?5MB'(+**G0,''L4155&&'%A;%>%&%%Zcp^ppp lSh^'[h`J.p,xpNC",l|:p$dKv)d&~ y-t,TpN]~50YM0$VP|/#N5*a|_*v)QLi/i<_]S*S)~sL$8`>Mur(1;d|o,4EA^ Ls-s8t%ddUr7I@Z Uxop? 7~#+pcF#sO'@<#W;&JT,-O@p^`)8p$} SY\8_pTvbR! r& d; U }&<J ] m''4^g:^{ yKA`tIW+4dJo8-<F>%1#'2C@E6G0)<,&0$/-7D<.#8K!1 #)$6&7,=B%'&5+Z5`\H5%-!c-(9,&Y01W#*H&. $"444!32&9&1%8/b^Tv#4'%P;9"1:9<.2%8)2<!,,L&+(Ph<S/YT,10$,O.\6,,3,!&98T,E15"0A/#&).o?3)715I's/&D;%38)4_,6%$L,@0 7C"#70B'"$G/-$,!&F%%*45 %E3<U56D#D'!7103?B.439F;'dA?#MY 39S>$:7.>9$)$bK.4#"+"?TT)C>>!9!0+/-;W #'""'"$D8J...[UW@4-7A3+!"UF-0F93"- 67N-=W ! ..KHGP2BmoknN08ZTM:y;5qfLSdQ1@Jf'T)f~~\tn`FqS;NA!>GAGGGGATTGTTTTGTGTTTTTAAATAGTGACACGGATAATAGCGTAGTACTCTATCTACACGGTTTAGTACACCCGGACCGTTAGTCTTATATCCGAGCTACAAATCCGACGATTATAGCGGCTTGAACAGCAGGAGAAGGAGCCCGCGCTGTCAGATCAGCACTATCCTCGTCATGGACAAAGTGTGCCGCGCAGGCATATTGTCCCTCGAGTCCTGAACGCCACGAAAGGCAGCGATTCGAGACAGCGGCCGGTGCAAAATGATACTATCAGTATATCGCACAAGCCGCCTCTTCTCTTTAAATACAAGAAGGAAGGGTCGTCATCCCCACGCCGGCCTCTGAAGGAAGTGATGACGGAAGACCTGACGTTCCAAGCTCTCTGATTAATGGAGAACCAAGTAATTCTTCAGAATAATTCGGGGGGACTCCCCGTTTTTTTTTCAGGAGGCATGTCGGGAATGATTCACTCCACGTATTCCGGCCTTTTCTTCCCATAAACTCCATGGTGGCGGGGGTTTTGATATATTTCGAGGGACATTGGTGAACCCAACACCAGCGTGAGTCCCGATTGGAAGAGTATTATAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-02 10:36:48 -04:00AEPt@AEPt@APFN T}APXV2APrN 0APrV1APrX}}0ARTNASPFASPtNASPtNB1PtMB1PtMBCTSrBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA >|(TDATA >|(ЮDATA >|(LDATA >|(DCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~qLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISE.P1AMM\cP1RLMWP1WDM`P2AMMj1P2BAMMnP2RLMePBASMMGPBASMMEaPCONMMU|PCONMMS/PDMFTfPDMFEEPLOCMNPLOCMIPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%E&SCAN@SMED SMLtSMPLTGSPACAinSPACE>SPACAinSVER4.0SVER rSVER,,/Scal@Scan@TUBED1Tmpr<UserphARphCHE\phDYbigphQLcphTRphTR4>This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN T}APXV2APrN 0APrV1APrX}}0ARTNASPFASPtNASPtNB1PtMB1PtMBCTSrBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA >|(TDATA >|(ЮDATA >|(LDATA >|(DCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVocFWO_GATCFeat//GTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~qLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISE.P1AMM\cP1RLMWP1WDM`P2AMMj1P2BAMMnP2RLMePBASMMGPBASMMEaPCONMMU|PCONMMS/PDMFTfPDMFEEPLOCMNPLOCMIPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%E&SCAN@SMED SMLtSMPLTGSPACAinSPACE>SPACAinSVER4.0SVER rSVER,,/Scal@Scan@TUBED1Tmpr<UserphARphCHE\phDYbigphQLcphTRphTRaB$T9F_z"\=QtAN-GQ6)lI PK&'Kxf]$bN8O2Rz7}062sXaA=&@X<1p3QLT1;g8kY+-^m]ZOQ>\G`HK_^_<~P[Z66YM'KvT+F4$U]]ReL#(79pA$den?R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb_v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r3oJ6l&O`x Pz><3&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hygS4-X;i+-'+1T:o5Q*oc0c3\EI`N^vp5cM_1h\j[<63*7wJ7{_O6?@dsgbk1^>7(FTc%{M*AqbEdG9}j~:HzpWI23Z;|T92JNMhhz rIIM'>bhjf8/FJrlM;8'i2{js.>`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6LPyr{;qe&Ker"&O93s'!byH:/U?&w;pQa+;]naFW^E)&jEp|9-$pVW5O@u+wGkSOj!Q4X+*flVinJils>:y;`4o_=&W.: wT_no2nJBY YlGcRb[%O8{q6.>`F7u2]H!R;{?kJ:hAC.F4/oak"T'o)6 K*G* bL1[ddKil.QqF3,.|] 2,\auBKD!K#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OL|(TDATA >|(ЮDATA >|(LDATA >|(DCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVocFWO_GATCFeat//GTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~qLIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISE.P1AMM\cP1RLMWP1WDM`P2AMMj1P2BAMMnP2RLMePBASMMGPBASMMEaPCONMMU|PCONMMS/PDMFTfPDMFEEPLOCMNPLOCMIPSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%E&SCAN@SMED SMLtSMPLTGSPACAinSPACE>SPACAinSVER4.0SVER rSVER,,/Scal@Scan@TUBED1Tmpr<UserphARphCHE\phDYbigphQLcphTRphTR