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%%,--444;;;BBBIIIOOOVVU\[[abagggmllsrrxww}||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6fc3b8bbb8b34b629cc946ab3dc3fb57Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-02_08-50_07223130Seq16779134781_134781mfagan96-Well########"#######"###""##########"########################"####"###"##"####""#"#######"######"###########################"########################"#########################"########################################"#"""##"""###################################"######"##"###"##########################################""######"###############"#########################"##################################################"####"#####"####"#######################################"#######"#############"##########################################"########"#################"#####################################################"#"####"#"###########"##################"#####"###########"#############################"####"################"###"#######################################"#"#"##"#####"###"###################"###############""#######"#############"################################################"######"#######################"############"######"##############################"##########"#######"############"######"################"############"#########################"#####"#"##################################################"##""###############################"##################################"######"##"######################################K1318_C12_Piper_sp_rbcL-FKB_3730_POP7_BDTv3.mobSeqPop7z 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"P=I5K"      8z;/q5 #   P\*^lNjy@     &Z"CB i;  RG`}F#      &Fs97dPfgMgf6~{5X/  .a4f{nCzG& ENnjArUgjAr{I]5    /hHsyYC  A|V~_!T/        4kK~Mp:  'V"SgY.i7 @xaidyp*`\M,HBRyt=ToA'      U>rp>rD'    NA\fs$Uh[2xF$     9a)9. e<     ;l0XaJzp-WeT)qC$      2Tm\e M{t@RtvZ'xQ8*$   C}Hhd@dfS}o;o|1k\ptSka7|N0    E.rh"haz05"b;      ;h}Y<'  5c OgbCf@'   *M O~zH2Yi_= qM3! =rF}q5pH1%             $Jz  c>"   =duq-?6pC$   !7Wh@" !;^^C/    *JqaA+ )?_zR3    ;[}jM5#           )Ek v[G7+!.Hk+)0@=* ]=%  3Rw |U6!       @jyV7       1Q~6lvJn]TRQOIA80+'%!  #0:ADB=5-&!                                                                              $=dAA!AGdAKB.bcpKB_3730_POP7_BDTv3.mobtermTATTGATTTGGGATCAAAGCTGGTGTTAAGATTACAAATTAACTTATTATACTCCTGAGTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCACCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCCTCTACTGGTACATGGACAAGTGTATGGACCGACGGACTTACCAGCCTTGATCGTTACAAAGGACGATGCTACGACATCGAGCCCGTTGCTGGAGAGGAAAATCAATATATTTGCTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCCGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGCTTCAAAGCCCTACGAGCCCTACGTCTGGAAGATCTACGAATCCCTCCTGCTTATTCCAAAACTTTCCAAGGCCCACCCCATGGAATCCAAGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCTCTATTGGGATGTACTATTAAACCAAAGTTAGGGTTATCGGCTAAGAACTACGGTAGGGCGGTTTATGAATGTCTCCGCGGTGGACTTGATTTTACTAAAATATTGATTTGGGATCAAAGCTGGTGTTAAGATTACAAATTAACTTATTATACTCCTGAGTATGAAACCAAAGATACTGATATCTTGGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCACCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCCTCTACTGGTACATGGACAAGTGTATGGACCGACGGACTTACCAGCCTTGATCGTTACAAAGGACGATGCTACGACATCGAGCCCGTTGCTGGAGAGGAAAATCAATATATTTGCTATGTAGCTTATCCTTTAGACCTTTTTGAAGAAGGTTCCGTTACTAACATGTTTACTTCCATTGTGGGTAATGTATTTGGCTTCAAAGCCCTACGAGCCCTACGTCTGGAAGATCTACGAATCCCTCCTGCTTATTCCAAAACTTTCCAAGGCCCACCCCATGGAATCCAAGTTGAAAGAGATAAATTGAACAAGTATGGTCGTCCTCTATTGGGATGTACTATTAAACCAAAGTTAGGGTTATCGGCTAAGAACTACGGTAGGGCGGTTTATGAATGTCTCCGCGGTGGACTTGATTTTACTAAAA #/6LSZbox-9FTdpx*9COZfs +4@LYfp{)5ALWdp| ".9ER`nx)4?KXcq}&3>JUany!,7BO\gt    * 6 A L X c o |   ! - 9 F S ] i v    ( 5 A M Y f r }  ! - 8 D P [ g r ~    ' 2 = I U a n z   ,7COZfr} %1<HTamy!,8DQ]kv*5BNZgr}%1=JVanz ",8DP\ht'3?JWep{ #0:FR^iv,8DP]hu'4?KXcp{ $0>JWbnz $0=IUamx ".:GS]gq~ #/6LSZbox-9FTdpx*9COZfs +4@LYfp{)5ALWdp| ".9ER`nx)4?KXcq}&3>JUany!,7BO\gt    * 6 A L X c o |   ! - 9 F S ] i v    ( 5 A M Y f r }  ! - 8 D P [ g r ~    ' 2 = I U a n z   ,7COZfr} %1<HTamy!,8DQ]kv*5BNZgr}%1=JVanz ",8DP\ht'3?JWep{ #0:FR^iv,8DP]hu'4?KXcp{ $0>JWbnz $0=IUamx ".:GS]gq~   ".1*(6##%-::*66::66&)>6>>>>>>>///8&>>>>>/ >>>;+>>>>;>>;>>>>>>;>>>>>>>>>;;>>;>>>>>>>>>>>>;1>>>>;>>>>;>;;>;>>>>;>>>>>>;;>;>>>>>>>>>>>>>;>;>>>;>>>>>>6>>>>>>>>>>>;>>>;>>>6;>68>;>;>>>>;;>>>>>>>>>>>>;;>>>>>>>>>;>;>>>>>>>>>>>>>6>>;;;>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>;;>>>>>>>>>>;>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>863>>>>8>>>>>>>>>>>>>>>>>>6>>>>>>>>>/3>>>>>3!&8#6>>>6>>8>>>>>>>>81>>>>>>>>>>>>6>>>>>>>4>>>>>6>:016::.::::6::6::1:::::::::6:::::::6:66::!::0:::5::46::3::::3:1/103::550&43:3/     ".1*(6##%-::*66::66&)>6>>>>>>>///8&>>>>>/ >>>;+>>>>;>>;>>>>>>;>>>>>>>>>;;>>;>>>>>>>>>>>>;1>>>>;>>>>;>;;>;>>>>;>>>>>>;;>;>>>>>>>>>>>>>;>;>>>;>>>>>>6>>>>>>>>>>>;>>>;>>>6;>68>;>;>>>>;;>>>>>>>>>>>>;;>>>>>>>>>;>;>>>>>>>>>>>>>6>>;;;>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>;;>>>>>>>>>>;>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>863>>>>8>>>>>>>>>>>>>>>>>>6>>>>>>>>>/3>>>>>3!&8#6>>>6>>8>>>>>>>>81>>>>>>>>>>>>6>>>>>>>4>>>>>6>:016::.::::6::6::1:::::::::6:::::::6:66::!::0:::5::46::3::::3:1/103::550&43:3/  H]U4b9NJME<G M+@ C@C>;, 4LNK=4Rc'=!8?5 S.W]),A /9[@I"VQ':D3ZG 8Ac"7,.!9-L$Y6=[$<S^JKY^dF.*6%^$D!'6BB., 8RJK#6B$#G*GA# <M:+Lb]\ ULC,_=^L5N$1<>`Q-M328D77I D+Y, ^6J8D')EYC^F^YTLO/3c5)5;A_/ <6$3 _7P9C0HDPW:# Q? $)#3;D[+IJMY4CFEPKXF=/W%:21R> : N!' ';\UL11_@90"'+I 0MWUG$C+ (O8<Q!P' T538%YCDV UV(^/]!M6/^%)ILc-V+N+.D&$8Y&(VG83=B"AFM>_0Z1LN&bM>\6*='5530P'N+AN? *9A<0 'H,=^6M;Nc][E!I66) 9`)R/Q;)$b2X- . 33%,E/!NcdD7:/(SI(Y(+8`\ *(_!B):!$! $@[Ow,`{u 92#T&}HXi70_1,QI}_r.HSDDsY  zTz'7yOBiAuhLEe#} 4o3/OQ 7-v.WVhxNai3C9S?(V;  U/6{'v3*0u#U|J]ohIJ:2Y9 l[~7U{uuf=iIS.jXv x0/_7|2dLR*v5Zm|h,*7_[y _n- <MZdb " Q) om*FI+q2Z[]GfeJWj4C5c# u7<\+R8^.7:LE#LV)TNv>8S;rTEN <eEK[Aswh``n>Ql"4.i  Q Ho.x  1_(y6SO"_Sq^RNVI?pVL=J5)/DV=/:4768%-9&                      ($#  &  "'!  46)3 0)"0$" A(E!$):()#?$7()&.(A% ,3E#,'>"+44-50E)9A8#11>HAB6F4??:6Q05H;C >:3-<GF2348.=RF=R=KKRpIa88@NH?8118511115F5555dGpppR<y,l0HBm,]91&9uZ8p8|pp8LYLdEV,3~PRPY9d,6 d,8Kdc$3PiI,tc%FU!i87zu ,f^dZMXG, L  ,6{Y2?"?MT'+6K4>^p?Z*`} "6-)@!0!00>pmO-*+?%!/3 &&+%(&*"7+8*3+822:09&;%#=/!"R=, )"2"!%,")7>5,"53& 0 ($!5.#5)*75 #0. )%$& * 8A",?./)&'00F-'"5"#D)')-4)&' 0178!3 #;5/1B#@5DKI4AO,!B+V=$8AF. +$8OF$!2!59X!*NJ+>6)D!%A D0!1G0/01-- #4< <H,$_S,22!@MA;&.$0%f"()$7++)32W9J*%Q;+9GvXF6;&'H" ,5 4)4k9SKH5K=XH}aM2Hs2'hN`o*3=oATAGTGGGGTTATAACCGAGGTTGTGATGATAATACCTAATCTCATAATATACTTCAGAATACGGCAACCGATATACCGATATCGTTACCGTAATTCGTATTACTTCTGGATTCAGAGCTACTGGCGGATGAGCACTCTCCGGAAGAGCAGGAGGATTCTCCAGATGTTCGCAGACGTGTGGGTAGATCGTTTTTCCCTTCATTCATTGTCGCCACGGAAGGAGATTCCTCTTCATCGCGGGCGCTTCCTGAGAAGGGCATCCAGGGAAACTCGCAAGAGCAGATTCAGGAGTTCCCCAAGGAGGAAGGGTCGATAATTTGATAAATTCCTTTAATGAAAATGAAGGGGGTTTAATCCGCTTTGTTCGATTTATTTGTGTAGGATATATAAGGATTTCTTGCTAACAATTCCCCAAAATTCCAATTTTTTTTTATTGGGTTCGAAAAGTGAGCTATGTAGCGGACGAGGGCCGGCCTTCTATGGCCCGAACTTCTGAGGCATCCGAAAGTTTAATATTATATGAGCAATGCCAGACATCAAAATAAGTAAGTCTTTTCTGTCTAGGTGAAGATAATTTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-02 10:38:07 -04:00AEPt@AEPt@APFN TxAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt\ASPt\B1Pt[B1Pt[BCTSIBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 9r TDATA 9r ơDATA 9r 8DATA 9r DCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~H5LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS P1AMC3P1RLC/TP1WDC8`P2AMCAlP2BACCEP2RLC<PBASCCPBASCC<PCONCC-PCONCC*PDMFTaPDMF PLOCC&HPLOCC!PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%SCAN@SMED SMLtSMPLTGSPACAmSPACSPACAmSVER4.0SVER ISVER,,/Scal@Scan@TUBEC12Tmpr<UserphARphCH7phDYbigphQLcphTRphTR6This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TxAPXV2APrN 0APrV1APrX}}0ARTNASPFASPt\ASPt\B1Pt[B1Pt[BCTSIBufT JCMNT 0lCTIDJ\CTNMJbCTOwJpCTTL 0cCpEPDATA@DATA@DATA@DATA@DATA DATA UDATA DATA %DATA 9r TDATA 9r ơDATA 9r 8DATA 9r DCHTDSamDySN 0DDye#DyeN0ODyeN0TDyeN0YDyeN0^DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabXFVocXFWO_GATCFeat//WGTyp!!0#HCFGCEHCFGHCFGHCFG==InScInVtLANE\LAST~~H5LIMS!!0yLNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS P1AMC3P1RLC/TP1WDC8`P2AMCAlP2BACCEP2RLC<PBASCCPBASCC<PCONCC-PCONCC*PDMFTaPDMF PLOCC&HPLOCC!PSZE`PTYPJwPXLBRGNmJTRMXV1RMdNB0RMdV1RMdXB@RPrNJ"RPrV1RUND RUND RUND RUND RUNT 2RUNT  $RUNT  $RUNT  $8Rate 0RunN""J2S/N%SCAN@SMED SMLtSMPLTGSPACAmSPACSPACAmSVER4.0SVER ISVER,,/Scal@Scan@TUBEC12Tmpr<UserphARphCH7phDYbigphQLcphTRphTRT7K4]O'T:cGave>u\nLTH@i=R/4zWvR'#]7&t1&^Ab{?nzF2}THOj-F'\/~X;Frxp/Ygnx'[vA-\K"L1{!Ulb|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+$#t{~FOI*aW$Aj#0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,,v1%$'#ttA4[>wXAr:3%6AB$+A?@*>-PM7M=a*N7lk;)+(H|XY+C}Q*xP!t p7YwV&=o>Z$~y;|>,m_noM$mOYePWz"$QAjat>9.b':8 v=iqNs)lktayr=u46_n&)-%Tw1(_CcSb;6AI8rBAr`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k_l_e5ll@i2zeqr[&X0VDE?g`p}CW5>QUStM4GmJhWQg,8p[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRbi`)lZFGz/X<7:?w5RiV1]cs:*?FvHQn: