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  $$$,,,554=<<DDDLKKSRRZZZ```gggmmltsuyy}~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2d45b5847ad64b969787fc93779f285fSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2016-07-07_10-50_07453130Seq16806134900_134900mfagan96-WellK1317_G8_Equisetum_sp_rbcL-RKB_3730_POP7_BDTv3.mobSeqPop7znVEJs`~vR)#UH \ImG 7$[VkUaSjF=EVfppj`WTYets]D'e= 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Hi3M;T_-;Tn]kvvq]}|wds{&>DS~d{]H;kWLwa3Oh#q#Ybi}Z@dw#m22q|4UJ\t^i<3_ho.Z!g~MZV3qb_Z\uSkH%o`t^U]xub<eI(pM]rxq_fkztM.\HAu)2fD$vaRr6fin *<K[xe3wr+w=G8_I+jffYr[be'CVd#k Fr[ "t;Ch/8xb9 V 9EGAAACGAAAAAATTCGCTCTTCAATTTTACGAACCGGGGCCATATATAAACGGTTAAATTAAACCTCCATAACGAGGGACCGCCGACATAAGCCAATTTAATCGCTCAAACCTGGAAGGCAAGGACCCGCACTAACACCGGTCCAACACGACCAGGCCGAATTCCACATTCCTCCAATTCTAATGTTGCGCGGTCTTAGAACCCTAACCCTTTACCAGCGAGGGGAGGAACCGGGCTAATACCCACACCTTCCTCCAGGGGGTCAGGGGGATGGCCTGCTACACGAACCATTTGCTTGCCATCCTAAACAAGCACACGGAGGAGAACACCTCCTCCTTCCATACAAAGAACGTGGATAAGCCCTTTCTCCCATCAGGCCATTATACACCAATAAAAAGTTCAGATATCAACGGCTTCCACATATTCGTGAGATAAACACTTATTTAAAAATTGAGGCACAACGGAAAGCAGGCTTACATCCGGGTACCTCGGGATCCATGACCGCATCTTACCAAATCCACCGCCCTCCCGCTCAACGACAAGGGAAACCAGGTCCAGAATTGGGTCCGGGAAAAGATAGTATACCCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82016-07-07 12:37:48 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTS{BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?8~pTDATA ?8~p0DATA ?8~pQDATA ?8~pDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FWO_GATCGTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE4LAST~~zLLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNP1AMKeP1RLKaP1WDKj?P2AMKskP2BAKKxP2RLKnPBASKKQPBASKKNPCONKK^PCONKK\}PDMFTPDMFNPLOCKWPLOCKSQPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%NSCAN@SMED SMLtSMPLTSPACAgSPACNSPACAgSVER4.0SVER {SVER,,/Scal@Scan@TUBEG8Tmpr<UserphARphCHNphDYbigphQLcphTRphTRHThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOB1PtNB1PtNBCTS{BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?8~pTDATA ?8~p0DATA ?8~pQDATA ?8~pDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab0FVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE4LAST~~zLLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNP1AMKeP1RLKaP1WDKj?P2AMKskP2BAKKxP2RLKnPBASKKQPBASKKNPCONKK^PCONKK\}PDMFTPDMFNPLOCKWPLOCKSQPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%NSCAN@SMED SMLtSMPLTSPACAgSPACNSPACAgSVER4.0SVER {SVER,,/Scal@Scan@TUBEG8Tmpr<UserphARphCHNphDYbigphQLcphTRphTRPF{ytl+hcDW4KuYGH[{O!QECN&0@]/0dwm\e+<}P?sw!iOup_3:E-v^gMs7M9{SHM6Fnf%)qk&YgDS7}_'AR;L6,8efj+l-uk#%C`]@zMxLrLei;E]yKoS]8qUvn}4|Mdna'"`ko`^ kg"#]eV&U)>`V2]<|':eqB?k3Pmr(vx+45B*5vh@|mcRFJ9'ngg61C"wY\V&Ip?Hds:MkO@JC)RVZW[Z!f]ZE!Q;eat6LS-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03SpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfF{\)lOY }YWoa$1pfnST/4f!Z56N|&ZG90Si!AEPt@AEPt@APFN TAPXV2APrN 0APrV1APrX}}0ARTNASPFASPtOASPtOAUDTCB1PtNB1PtNBCTS{BufT JCMNT 0CTIDJCTNMJCTOwJCTTL 0CpEPDATA@DATA@DATA@DATA@DATA DATA aDATA 5DATA & DATA ?8~pTDATA ?8~p0DATA ?8~pQDATA ?8~pDCHTDSamDySN 0tDye#DyeN0DyeN0DyeN0DyeN0DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0 EVNT 0EVNT0!EVNT04FTab0FVocFWO_GATCFeat//GTyp!!0SHCFGCEHCFGHCFGHCFG==InScInVtLANE4LAST~~zLLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISNP1AMKeP1RLKaP1WDKj?P2AMKskP2BAKKxP2RLKnPBASKKQPBASKKNPCONKK^PCONKK\}PDMFTPDMFNPLOCKWPLOCKSQPSZE`PTYPJPXLBRGNmJRMXV1RMdNB`RMdV1RMdXBpRPrNJRRPrV1RUND RUND RUND RUND RUNT  2RUNT  $7RUNT  RUNT  $.Rate 0GRunN""JbS/N%NSCAN@SMED SMLtSMPLTSPACAgSPACNSPACAgSVER4.0SVER {SVER,,/Scal@Scan@TUBEG8Tmpr<UserphARphCHNphDYbigphQLcphTRphTR